{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362262944579660416.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/j.cellimm.2009.10.011"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0008874909001920?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0008874909001920?httpAccept=text/plain"}},{"identifier":{"@type":"PMID","@value":"19932892"}}],"dc:title":[{"@value":"Complexin II regulates degranulation in RBL-2H3 cells by interacting with SNARE complex containing syntaxin-3"}],"description":[{"notation":[{"@value":"Recent studies have revealed that SNARE proteins are involved in the exocytotic release (degranulation) in mast cells. However, the roles of SNARE regulatory proteins are poorly understood. Complexin is one such regulatory protein and it plays a crucial role in exocytotic release. In this study, we characterized the interaction between SNARE complex and complexin II in mast cells by GST pull-down assay and in vitro binding assay. We found that the SNARE complex that interacted with complexin II consisted of syntaxin-3, SNAP-23, and VAMP-2 or -8, whereas syntaxin-4 was not detected. Recombinant syntaxin-3 binds to complexin II by itself, but its affinity to complexin II was enhanced upon addition of VAMP-8 and SNAP-23. Furthermore, the region of complexin II responsible for binding to the SNARE complex, was near the central alpha-helix region. These results suggest that complexin II regulates degranulation by interacting with the SNARE complex containing syntaxin-3."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382262944579660418","@type":"Researcher","foaf:name":[{"@value":"Satoshi Tadokoro"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944579660416","@type":"Researcher","foaf:name":[{"@value":"Mamoru Nakanishi"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944579660417","@type":"Researcher","foaf:name":[{"@value":"Naohide Hirashima"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00088749"}],"prism:publicationName":[{"@value":"Cellular Immunology"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"2010","prism:volume":"261","prism:number":"1","prism:startingPage":"51","prism:endingPage":"56"},"reviewed":"false","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/","https://www.elsevier.com/legal/tdmrep-license"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S0008874909001920?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S0008874909001920?httpAccept=text/plain"}],"createdAt":"2009-10-26","modifiedAt":"2025-11-02","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Binding%20Sites","dc:title":"Binding Sites"},{"@id":"https://cir.nii.ac.jp/all?q=Qa-SNARE%20Proteins","dc:title":"Qa-SNARE Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Nerve%20Tissue%20Proteins","dc:title":"Nerve Tissue Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Exocytosis","dc:title":"Exocytosis"},{"@id":"https://cir.nii.ac.jp/all?q=Rats","dc:title":"Rats"},{"@id":"https://cir.nii.ac.jp/all?q=Adaptor%20Proteins,%20Vesicular%20Transport","dc:title":"Adaptor Proteins, Vesicular Transport"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Line,%20Tumor","dc:title":"Cell Line, Tumor"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=SNARE%20Proteins","dc:title":"SNARE Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Protein%20Binding","dc:title":"Protein Binding"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004231995949568","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Effects of synaptotagmin 2 on membrane fusion between liposomes that contain SNAREs involved in exocytosis in mast cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360290617731089152","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Rab44 isoforms similarly promote lysosomal exocytosis, but exhibit differential localization in mast cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567181948312704","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Biosurfactant mannosyl-erythritol lipid inhibits secretion of inflammatory mediators from RBL-2H3 cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360846643592983680","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Distinct Initial SNARE Configurations Underlying the Diversity of Exocytosis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848656926194176","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Effects of PIP2 on membrane fusion between mast cell SNARE liposomes mediated by synaptotagmin 2"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204632869248","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"UDP/P2Y6 receptor signaling regulates IgE-dependent degranulation in human basophils"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679609557120","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Effect of Complexin II on Membrane Fusion between Liposomes Containing Mast Cell SNARE Proteins"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1016/j.cellimm.2009.10.011"},{"@type":"OPENAIRE","@value":"doi_dedup___::9a0f4e7896fe726dc80472988e653597"},{"@type":"CROSSREF","@value":"10.1002/2211-5463.13133_references_DOI_ZEEwOxgtuyTlnAGLCkZgiglXWJ"},{"@type":"CROSSREF","@value":"10.1016/j.bbamem.2015.06.016_references_DOI_ZEEwOxgtuyTlnAGLCkZgiglXWJ"},{"@type":"CROSSREF","@value":"10.1016/j.bbamem.2011.07.003_references_DOI_ZEEwOxgtuyTlnAGLCkZgiglXWJ"},{"@type":"CROSSREF","@value":"10.1152/physrev.00007.2012_references_DOI_ZEEwOxgtuyTlnAGLCkZgiglXWJ"},{"@type":"CROSSREF","@value":"10.1016/j.bbagen.2011.07.002_references_DOI_ZEEwOxgtuyTlnAGLCkZgiglXWJ"},{"@type":"CROSSREF","@value":"10.1248/bpb.b15-00751_references_DOI_ZEEwOxgtuyTlnAGLCkZgiglXWJ"},{"@type":"CROSSREF","@value":"10.1016/j.alit.2017.02.014_references_DOI_ZEEwOxgtuyTlnAGLCkZgiglXWJ"}]}