{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362262944585910784.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1007/s00249-008-0264-5"}},{"identifier":{"@type":"URI","@value":"http://link.springer.com/content/pdf/10.1007/s00249-008-0264-5.pdf"}},{"identifier":{"@type":"URI","@value":"http://link.springer.com/article/10.1007/s00249-008-0264-5/fulltext.html"}},{"identifier":{"@type":"URI","@value":"http://link.springer.com/content/pdf/10.1007/s00249-008-0264-5"}},{"identifier":{"@type":"PMID","@value":"18259741"}}],"dc:title":[{"@value":"Development of phase plates for electron microscopes and their biological application"}],"description":[{"notation":[{"@value":"After slow progress in the efforts to develop phase plates for electron microscopes, functional phase plates with thin carbon films have recently been reported. An electron microscope enhanced with thin-film phase plates has practical advantages. It permits collecting high-contrast images of intact biological specimens without harsh and lengthy sample preparation, such as fixation, dehydration, resin-embedding, staining and thin-sectioning. This report reviews the state of the art for phase plates in biological electron microscopy and focuses upon the conditions required for functional thin-film phase plates. The current disadvantages of thin-film phase plates are also addressed and potential solutions are proposed."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382262944585910784","@type":"Researcher","foaf:name":[{"@value":"Kuniaki Nagayama"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"01757571"},{"@type":"EISSN","@value":"14321017"}],"prism:publicationName":[{"@value":"European Biophysics Journal"}],"dc:publisher":[{"@value":"Springer Science and Business Media LLC"}],"prism:publicationDate":"2008-02-08","prism:volume":"37","prism:number":"4","prism:startingPage":"345","prism:endingPage":"358"},"reviewed":"false","dc:rights":["http://www.springer.com/tdm"],"url":[{"@id":"http://link.springer.com/content/pdf/10.1007/s00249-008-0264-5.pdf"},{"@id":"http://link.springer.com/article/10.1007/s00249-008-0264-5/fulltext.html"},{"@id":"http://link.springer.com/content/pdf/10.1007/s00249-008-0264-5"}],"createdAt":"2008-02-07","modifiedAt":"2024-02-22","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Models,%20Statistical","dc:title":"Models, Statistical"},{"@id":"https://cir.nii.ac.jp/all?q=Cryoelectron%20Microscopy","dc:title":"Cryoelectron Microscopy"},{"@id":"https://cir.nii.ac.jp/all?q=Static%20Electricity","dc:title":"Static Electricity"},{"@id":"https://cir.nii.ac.jp/all?q=Electrons","dc:title":"Electrons"},{"@id":"https://cir.nii.ac.jp/all?q=Equipment%20Design","dc:title":"Equipment Design"},{"@id":"https://cir.nii.ac.jp/all?q=Carbon","dc:title":"Carbon"},{"@id":"https://cir.nii.ac.jp/all?q=Microscopy,%20Electron,%20Transmission","dc:title":"Microscopy, Electron, Transmission"},{"@id":"https://cir.nii.ac.jp/all?q=Image%20Processing,%20Computer-Assisted","dc:title":"Image Processing, Computer-Assisted"},{"@id":"https://cir.nii.ac.jp/all?q=Microscopy,%20Phase-Contrast","dc:title":"Microscopy, Phase-Contrast"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360861289354134272","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Complex Observation in Electron Microscopy VIII: Novel Hilbert Phase Plates to Maximize Phase Contrast"}]},{"@id":"https://cir.nii.ac.jp/crid/1390588147237686912","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"The future of Asian biophysics built through Korea–Japan collaboration"}]},{"@id":"https://cir.nii.ac.jp/crid/1520573330749468416","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Complex observation in electron microscopy (7) Iterative phase retrieval for strong-phase objects by plural Hilbert differential contrast experiments"},{"@language":"ja-Kana","@value":"Complex observation in electron microscopy 7 Iterative phase retrieval for strong phase objects by plural Hilbert differential contrast experiments"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1007/s00249-008-0264-5"},{"@type":"OPENAIRE","@value":"doi_dedup___::83e1a251700aab98473b03eeaf6c7909"},{"@type":"CROSSREF","@value":"10.7566/jpsj.92.074402_references_DOI_a12yFspO40CFLNx0Yfv0UOXIQwO"},{"@type":"CROSSREF","@value":"10.2142/biophysico.bppb-v22.s004_references_DOI_a12yFspO40CFLNx0Yfv0UOXIQwO"},{"@type":"CROSSREF","@value":"10.1143/jpsj.80.094402_references_DOI_a12yFspO40CFLNx0Yfv0UOXIQwO"}]}