{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362262944632052864.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1182/blood.v82.2.456.456"}},{"identifier":{"@type":"URI","@value":"http://ashpublications.org/blood/article-pdf/82/2/456/611049/456.pdf"}}],"dc:title":[{"@value":"Establishment and characterization of an erythropoietin-dependent subline, UT-7/Epo, derived from human leukemia cell line, UT-7"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title>\n               <jats:p>UT-7 is a human leukemic cell line capable of growing in interleukin-3 (IL-3), granulocyte/macrophage colony-stimulating factor (GM-CSF), or erythropoietin (Epo) (Komatsu et al, Cancer Res 51:341, 1991). To study the effect of Epo on proliferation and differentiation of UT-7, we maintained the UT-7 cell culture for more than 6 months in the presence of Epo. As a result, a subline, UT-7/Epo, was established. The growth of UT-7/Epo could be supported by Epo but not by GM-CSF or IL-3. UT- 7/Epo showed a greater level of heme content and ratio of benzidine- positive staining cells than did UT-7. Butyric acid promoted the synthesis of hemoglobin in UT-7/Epo, but not UT-7. Further, the mRNA concentrations of the c-myb oncogene and GM-CSF receptor beta-subunit were decreased substantially in UT-7/Epo cells. These findings showed that UT-7/Epo cells had progressed further in erythroid development than UT-7 cells, and suggested that long-term culture in Epo had promoted this differentiation. Whereas availability of the Epo receptor (Epo-R) for binding of Epo was reduced in UT-7/Epo cells compared with UT-7 cells, the Epo-R showed a similar affinity for Epo. This observation suggested that change(s) in postreceptor signaling step might be involved in the establishment and maintenance of the UT-7/Epo phenotype.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380004237579335051","@type":"Researcher","foaf:name":[{"@value":"N Komatsu"}],"jpcoar:affiliationName":[{"@value":"Department of Medicine, Jichi Medical School, Tochigi, Japan."}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944632052736","@type":"Researcher","foaf:name":[{"@value":"M Yamamoto"}],"jpcoar:affiliationName":[{"@value":"Department of Medicine, Jichi Medical School, Tochigi, Japan."}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944632052864","@type":"Researcher","foaf:name":[{"@value":"H Fujita"}],"jpcoar:affiliationName":[{"@value":"Department of Medicine, Jichi Medical School, Tochigi, Japan."}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944632052737","@type":"Researcher","foaf:name":[{"@value":"A Miwa"}],"jpcoar:affiliationName":[{"@value":"Department of Medicine, Jichi Medical School, Tochigi, Japan."}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944632052741","@type":"Researcher","foaf:name":[{"@value":"K Hatake"}],"jpcoar:affiliationName":[{"@value":"Department of Medicine, Jichi Medical School, Tochigi, Japan."}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944632052740","@type":"Researcher","foaf:name":[{"@value":"T Endo"}],"jpcoar:affiliationName":[{"@value":"Department of Medicine, Jichi Medical School, Tochigi, Japan."}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944632052866","@type":"Researcher","foaf:name":[{"@value":"H Okano"}],"jpcoar:affiliationName":[{"@value":"Department of Medicine, Jichi Medical School, Tochigi, Japan."}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944632052867","@type":"Researcher","foaf:name":[{"@value":"T Katsube"}],"jpcoar:affiliationName":[{"@value":"Department of Medicine, Jichi Medical School, Tochigi, Japan."}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944632052738","@type":"Researcher","foaf:name":[{"@value":"Y Fukumaki"}],"jpcoar:affiliationName":[{"@value":"Department of Medicine, Jichi Medical School, Tochigi, Japan."}]},{"@id":"https://cir.nii.ac.jp/crid/1382262944632052739","@type":"Researcher","foaf:name":[{"@value":"S Sassa"}],"jpcoar:affiliationName":[{"@value":"Department of Medicine, Jichi Medical School, Tochigi, Japan."}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00064971"},{"@type":"EISSN","@value":"15280020"}],"prism:publicationName":[{"@value":"Blood"}],"dc:publisher":[{"@value":"American Society of Hematology"}],"prism:publicationDate":"1993-07-15","prism:volume":"82","prism:number":"2","prism:startingPage":"456","prism:endingPage":"464"},"reviewed":"false","url":[{"@id":"http://ashpublications.org/blood/article-pdf/82/2/456/611049/456.pdf"}],"createdAt":"2019-10-14","modifiedAt":"2019-11-20","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050001335827064192","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"H-Ferritin Is Preferentially Incorporated by Human Erythroid Cells through Transferrin Receptor 1 in a Threshold-Dependent Manner."}]},{"@id":"https://cir.nii.ac.jp/crid/1050282810836926208","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Novel TPO receptor agonist TA-316 contributes to platelet biogenesis from human iPS 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