{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362262945652452736.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/j.ijpharm.2004.03.013"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0378517304002017?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0378517304002017?httpAccept=text/plain"}},{"identifier":{"@type":"PMID","@value":"15196630"}}],"dc:title":[{"@value":"In vivo gene transfection via intravitreal injection of cationic liposome/plasmid DNA complexes in rabbits"}],"description":[{"notation":[{"@value":"To optimize the in vivo ocular transfection efficiency of plasmid DNA (pDNA)/cationic liposome complexes, N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA)/dioleoylphosphatidylethanolamine (DOPE) (1:1 molar ratio) liposomes and DOTMA/cholesterol (Chol) (1:1 molar ratio) liposomes were prepared with varying amounts of pDNA. pDNA/cationic liposome complexes were intravitreally injected (100 microL) in rabbits, and luciferase activity in the cornea, aqueous humor, iris-ciliary body, lens, vitreous body, and retina was measured. Transfection efficiency of pDNA alone did not change with pDNA ranging from 40 to 85 mg. In contrast, transfection efficiency of pDNA complexed with DOTMA/Chol liposomes significantly increased with the amount of pDNA ranging from 40 to 85 microg (P0.05). pDNA complexed with DOTMA/DOPE liposomes could not be prepared with pDNA greater than 60 microg. Among these experiments, pDNA (85 microg) complexed with DOTMA/Chol liposomes (pDNA:cationic liposome charge ratio (- : +) = 1.0:2.0) showed the highest transfection efficiency in the ocular tissue and its transfection-mediated luciferase activity peaked at 3 days. Among the ocular tissues, the highest gene expression was observed in the aqueous humor."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382262945652452741","@type":"Researcher","foaf:name":[{"@value":"Shigeru Kawakami"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262945652452744","@type":"Researcher","foaf:name":[{"@value":"Ayaka Harada"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262945652452740","@type":"Researcher","foaf:name":[{"@value":"Koji Sakanaka"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262945652452738","@type":"Researcher","foaf:name":[{"@value":"Koyo Nishida"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262945652452742","@type":"Researcher","foaf:name":[{"@value":"Junzo Nakamura"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262945652452743","@type":"Researcher","foaf:name":[{"@value":"Toshiyuki Sakaeda"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262945652452736","@type":"Researcher","foaf:name":[{"@value":"Nobuhiro Ichikawa"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262945652452737","@type":"Researcher","foaf:name":[{"@value":"Mikiro Nakashima"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262945652452739","@type":"Researcher","foaf:name":[{"@value":"Hitoshi Sasaki"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"03785173"},{"@type":"PISSN","@value":"https://id.crossref.org/issn/03785173"}],"prism:publicationName":[{"@value":"International Journal of Pharmaceutics"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"2004-07","prism:volume":"278","prism:number":"2","prism:startingPage":"255","prism:endingPage":"262"},"reviewed":"false","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/","https://www.elsevier.com/legal/tdmrep-license"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S0378517304002017?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S0378517304002017?httpAccept=text/plain"}],"createdAt":"2004-05-14","modifiedAt":"2025-10-08","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Male","dc:title":"Male"},{"@id":"https://cir.nii.ac.jp/all?q=Phosphatidylethanolamines","dc:title":"Phosphatidylethanolamines"},{"@id":"https://cir.nii.ac.jp/all?q=DNA","dc:title":"DNA"},{"@id":"https://cir.nii.ac.jp/all?q=Transfection","dc:title":"Transfection"},{"@id":"https://cir.nii.ac.jp/all?q=Quaternary%20Ammonium%20Compounds","dc:title":"Quaternary Ammonium Compounds"},{"@id":"https://cir.nii.ac.jp/all?q=Vitreous%20Body","dc:title":"Vitreous Body"},{"@id":"https://cir.nii.ac.jp/all?q=Cholesterol","dc:title":"Cholesterol"},{"@id":"https://cir.nii.ac.jp/all?q=Cations","dc:title":"Cations"},{"@id":"https://cir.nii.ac.jp/all?q=Liposomes","dc:title":"Liposomes"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Rabbits","dc:title":"Rabbits"},{"@id":"https://cir.nii.ac.jp/all?q=Luciferases","dc:title":"Luciferases"},{"@id":"https://cir.nii.ac.jp/all?q=Plasmids","dc:title":"Plasmids"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1390001204171294464","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Lipid Carrier Systems for Targeted Drug and Gene Delivery"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001277392367360","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Adsorption Phenomena of Anionic and Cationic Nanoliposomes on the Surface of Poly(dimethylsiloxane) Microchannel"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679610014080","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Ocular Gene Delivery Systems Using Ternary Complexes of Plasmid DNA, Polyethylenimine, and Anionic Polymers"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1016/j.ijpharm.2004.03.013"},{"@type":"OPENAIRE","@value":"doi_dedup___::1a945eeb6dd23d2b4e6eb7021186800e"},{"@type":"CROSSREF","@value":"10.1248/bpb.b12-00728_references_DOI_9jycGE83aA1CxhQYvzRZK6vFVT7"},{"@type":"CROSSREF","@value":"10.1248/cpb.53.871_references_DOI_9jycGE83aA1CxhQYvzRZK6vFVT7"},{"@type":"CROSSREF","@value":"10.2494/photopolymer.32.107_references_DOI_9jycGE83aA1CxhQYvzRZK6vFVT7"}]}