{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362262946029447296.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1128/jcm.34.1.49-54.1996"}},{"identifier":{"@type":"URI","@value":"https://journals.asm.org/doi/pdf/10.1128/jcm.34.1.49-54.1996"}}],"dc:title":[{"@value":"Simultaneous PCR detection of Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus types 1 and 2 from genital ulcers"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>A multiplex PCR (M-PCR) assay with colorimetric detection was devised for the simultaneous amplification of DNA targets from Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus (HSV) types 1 and 2. By using target-specific oligonucleotides in a microwell format, 298 genital ulcer swab specimens collected in New Orleans during three intervals from 1992 through 1994 were evaluated. The results of the M-PCR assay were compared with the results of dark-field microscopy and H. ducreyi culture on two different culture media. HSV culture results were available for 99 specimens collected during the third interval. Confirmatory PCR assays targeting different gene sequences for each of the three organisms were used to validate the M-PCR results. Specimens were resolved as positive for the determination of sensitivity if the reference diagnostic test was positive or if the results of both the M-PCR and the confirmatory PCR were positive. The resolved sensitivities of M-PCR for HSV, H. ducreyi, and T. pallidum were 100, 98.4, and 91%, respectively. The resolved sensitivities of HSV culture, H. ducreyi culture, and dark-field microscopy were 71.8, 74.2, and 81%, respectively. These results indicate that the M-PCR assay is more sensitive than standard diagnostic tests for the detection of HSV, H. ducreyi, and T. pallidum from genital ulcers.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1381975846708787074","@type":"Researcher","foaf:name":[{"@value":"K A Orle"}],"jpcoar:affiliationName":[{"@value":"Roche Molecular Systems, Alameda, California 94501, USA. karina.orle@roche.com"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262946029447299","@type":"Researcher","foaf:name":[{"@value":"C A Gates"}],"jpcoar:affiliationName":[{"@value":"Roche Molecular Systems, Alameda, California 94501, USA. karina.orle@roche.com"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262946029447298","@type":"Researcher","foaf:name":[{"@value":"D H Martin"}],"jpcoar:affiliationName":[{"@value":"Roche Molecular Systems, Alameda, California 94501, USA. karina.orle@roche.com"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262946029447297","@type":"Researcher","foaf:name":[{"@value":"B A Body"}],"jpcoar:affiliationName":[{"@value":"Roche Molecular Systems, Alameda, California 94501, USA. karina.orle@roche.com"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262946029447300","@type":"Researcher","foaf:name":[{"@value":"J B Weiss"}],"jpcoar:affiliationName":[{"@value":"Roche Molecular Systems, Alameda, California 94501, USA. karina.orle@roche.com"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00951137"},{"@type":"EISSN","@value":"1098660X"}],"prism:publicationName":[{"@value":"Journal of Clinical Microbiology"}],"dc:publisher":[{"@value":"American Society for Microbiology"}],"prism:publicationDate":"1996-01","prism:volume":"34","prism:number":"1","prism:startingPage":"49","prism:endingPage":"54"},"reviewed":"false","dc:rights":["https://journals.asm.org/non-commercial-tdm-license"],"url":[{"@id":"https://journals.asm.org/doi/pdf/10.1128/jcm.34.1.49-54.1996"}],"createdAt":"2020-01-06","modifiedAt":"2022-03-05","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004235982845056","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Circulation of Distinct\n            <i>Treponema pallidum</i>\n            Strains in Individuals with Heterosexual Orientation and Men Who Have Sex with Men"}]},{"@id":"https://cir.nii.ac.jp/crid/1360009142799703040","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Epidemiology, molecular strain types, and macrolide resistance of Treponema pallidum in Japan, 2017–2018"}]},{"@id":"https://cir.nii.ac.jp/crid/1360022304970870400","@type":"Article","resourceType":"preprint","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Molecular typing and macrolide resistance analyses of\n                  <i>Treponema pallidum</i>\n                  in heterosexuals and men who have sex with men communities in Japan, 2017"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285710959560960","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Molecular Typing and Macrolide Resistance Analyses of Treponema pallidum in Heterosexuals and Men Who Have Sex with Men in Japan, 2017"}]},{"@id":"https://cir.nii.ac.jp/crid/1361975846708786944","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A lung abscess caused by secondary syphilis – the utility of polymerase chain reaction techniques in transbronchial biopsy: a case report"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1128/jcm.34.1.49-54.1996"},{"@type":"CROSSREF","@value":"10.1128/jcm.01148-18_references_DOI_8NEhzsy7Z0cTbeLniUJ57BsmdhB"},{"@type":"CROSSREF","@value":"10.1101/375485_references_DOI_2tHZZLur6wahmemSuWpK2pDPoHa"},{"@type":"CROSSREF","@value":"10.1016/j.jiac.2020.05.022_references_DOI_8NEhzsy7Z0cTbeLniUJ57BsmdhB"},{"@type":"CROSSREF","@value":"10.1128/jcm.01167-18_references_DOI_8NEhzsy7Z0cTbeLniUJ57BsmdhB"},{"@type":"CROSSREF","@value":"10.1186/s12879-019-4236-4_references_DOI_2tHZZLur6wahmemSuWpK2pDPoHa"}]}