{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362262946136352256.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1152/ajpregu.00483.2006"}},{"identifier":{"@type":"URI","@value":"https://www.physiology.org/doi/pdf/10.1152/ajpregu.00483.2006"}}],"dc:title":[{"@value":"Denervation does not change the ratio of collagen I and collagen III mRNA in the extracellular matrix of muscle"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>Denervation or inactivity is known to decrease the mass and alter the phenotype of muscle and the mechanics of tendon. It has been proposed that a shift in the collagen of the extracellular matrix (ECM) of the muscle, increasing type III and decreasing type I collagen, may be partially responsible for the observed changes. We directly investigated this hypothesis using quantitative real-time PCR on muscles and tendons that had been denervated for 5 wk. Five weeks of denervation resulted in a 2.91-fold increase in collagen concentration but no change in the content of collagen in the muscle, whereas in the tendon there was no change in either the concentration or content of collagen. The expression of collagen I, collagen III, and lysyl oxidase mRNA in the ECM of muscle decreased (76 ± 1.6%, 73 ± 2.3%, and 83 ± 3.2%, respectively) after 5 wk of denervation. Staining with picrosirius red confirmed the earlier observation of a change in staining color from red to green. Taken with the observed equivalent decreases in collagen I and III mRNA, this suggests that there was a change in orientation of the ECM of muscle becoming more aligned with the axis of the muscle fibers and no change in collagen type. The change in collagen orientation may serve to protect the smaller muscle fibers from damage by increasing the stiffness of the ECM and may partly explain why the region of the tendon closest to the muscle becomes stiffer after inactivity.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382262946136352257","@type":"Researcher","foaf:name":[{"@value":"Ellen M. Arruda"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262946136352384","@type":"Researcher","foaf:name":[{"@value":"Kevin Mundy"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262946136352385","@type":"Researcher","foaf:name":[{"@value":"Sarah Calve"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262946136352256","@type":"Researcher","foaf:name":[{"@value":"Keith Baar"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"03636119"},{"@type":"EISSN","@value":"15221490"}],"prism:publicationName":[{"@value":"American Journal of Physiology-Regulatory, Integrative and Comparative Physiology"}],"dc:publisher":[{"@value":"American Physiological Society"}],"prism:publicationDate":"2007-02","prism:volume":"292","prism:number":"2","prism:startingPage":"R983","prism:endingPage":"R987"},"reviewed":"false","url":[{"@id":"https://www.physiology.org/doi/pdf/10.1152/ajpregu.00483.2006"}],"createdAt":"2006-09-29","modifiedAt":"2020-04-18","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360009142744457600","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Fast repetitive stretch suppresses denervation‐induced muscle fibrosis"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204901825664","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Regulation of connective tissue remodeling in the early phase of denervation in a rat skeletal muscle"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1152/ajpregu.00483.2006"},{"@type":"CROSSREF","@value":"10.1002/mus.27059_references_DOI_2uGjzMCaEwyNL8W8Abprc0f9n79"},{"@type":"CROSSREF","@value":"10.2220/biomedres.34.251_references_DOI_2uGjzMCaEwyNL8W8Abprc0f9n79"}]}