{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362262946353783424.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1104/pp.109.145094"}},{"identifier":{"@type":"URI","@value":"http://academic.oup.com/plphys/article-pdf/151/3/1421/37114425/plphys_v151_3_1421.pdf"}}],"dc:title":[{"@value":"Functional Analysis of<i>α</i>-DOX2, an Active<i>α</i>-Dioxygenase Critical for Normal Development in Tomato Plants"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title><jats:p>Plant α-dioxygenases initiate the synthesis of oxylipins by catalyzing the incorporation of molecular oxygen at the α-methylene carbon atom of fatty acids. Previously, α-DOX1 has been shown to display α-dioxygenase activity and to be implicated in plant defense. In this study, we investigated the function of a second α-dioxygenase isoform, α-DOX2, in tomato (Solanum lycopersicum) and Arabidopsis (Arabidopsis thaliana). Recombinant Slα-DOX2 and Atα-DOX2 proteins catalyzed the conversion of a wide range of fatty acids into 2(R)-hydroperoxy derivatives. Expression of Slα-DOX2 and Atα-DOX2 was found in seedlings and increased during senescence induced by detachment of leaves. In contrast, microbial infection, earlier known to increase the expression of α-DOX1, did not alter the expression of Slα-DOX2 or Atα-DOX2. The tomato mutant divaricata, characterized by early dwarfing and anthocyanin accumulation, carries a mutation at the Slα-DOX2 locus and was chosen for functional studies of α-DOX2. Transcriptional changes in such mutants showed the up-regulation of genes playing roles in lipid and phenylpropanoid metabolism, the latter being in consonance with the anthocyanin accumulation. Transgenic expression of Atα-DOX2 and Slα-DOX2 in divaricata partially complemented the compromised phenotype in mature plants and fully complemented it in seedlings, thus indicating the functional exchangeability between α-DOX2 from tomato and Arabidopsis. However, deletion of Atα-DOX2 in Arabidopsis plants did not provoke any visible phenotypic alteration indicating that the relative importance of α-DOX2 in plant physiology is species specific.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382262946353783426","@type":"Researcher","foaf:name":[{"@value":"Gerard Bannenberg"}],"jpcoar:affiliationName":[{"@value":"Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, 28049 Madrid, Spain (G.B., M.M., M.J.R., M.A.L., C.C.); Departamento Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, 11600 Montevideo, Uruguay (I.P.L.); and Department of Medical Biochemistry and Biophysics, Division of Physiological"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262946353783428","@type":"Researcher","foaf:name":[{"@value":"Marta Martínez"}],"jpcoar:affiliationName":[{"@value":"Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, 28049 Madrid, Spain (G.B., M.M., M.J.R., M.A.L., C.C.); Departamento Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, 11600 Montevideo, Uruguay (I.P.L.); and Department of Medical Biochemistry and Biophysics, Division of Physiological"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262946353783425","@type":"Researcher","foaf:name":[{"@value":"María José Rodríguez"}],"jpcoar:affiliationName":[{"@value":"Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, 28049 Madrid, Spain (G.B., M.M., M.J.R., M.A.L., C.C.); Departamento Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, 11600 Montevideo, Uruguay (I.P.L.); and Department of Medical Biochemistry and Biophysics, Division of Physiological"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262946353783430","@type":"Researcher","foaf:name":[{"@value":"Miguel Angel López"}],"jpcoar:affiliationName":[{"@value":"Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, 28049 Madrid, Spain (G.B., M.M., M.J.R., M.A.L., C.C.); Departamento Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, 11600 Montevideo, Uruguay (I.P.L.); and Department of Medical Biochemistry and Biophysics, Division of Physiological"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262946353783429","@type":"Researcher","foaf:name":[{"@value":"Inés Ponce de León"}],"jpcoar:affiliationName":[{"@value":"Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, 28049 Madrid, Spain (G.B., M.M., M.J.R., M.A.L., C.C.); Departamento Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, 11600 Montevideo, Uruguay (I.P.L.); and Department of Medical Biochemistry and Biophysics, Division of Physiological"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262946353783424","@type":"Researcher","foaf:name":[{"@value":"Mats Hamberg"}],"jpcoar:affiliationName":[{"@value":"Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, 28049 Madrid, Spain (G.B., M.M., M.J.R., M.A.L., C.C.); Departamento Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, 11600 Montevideo, Uruguay (I.P.L.); and Department of Medical Biochemistry and Biophysics, Division of Physiological"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262946353783427","@type":"Researcher","foaf:name":[{"@value":"Carmen Castresana"}],"jpcoar:affiliationName":[{"@value":"Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, 28049 Madrid, Spain (G.B., M.M., M.J.R., M.A.L., C.C.); Departamento Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, 11600 Montevideo, Uruguay (I.P.L.); and Department of Medical Biochemistry and Biophysics, Division of Physiological"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"15322548"}],"prism:publicationName":[{"@value":"Plant Physiology"}],"dc:publisher":[{"@value":"Oxford University Press (OUP)"}],"prism:publicationDate":"2009-09-16","prism:volume":"151","prism:number":"3","prism:startingPage":"1421","prism:endingPage":"1432"},"reviewed":"false","dc:rights":["https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model"],"url":[{"@id":"http://academic.oup.com/plphys/article-pdf/151/3/1421/37114425/plphys_v151_3_1421.pdf"}],"createdAt":"2009-09-17","modifiedAt":"2025-02-12","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050282810793045888","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Leaf oil bodies are subcellular factories producing antifungal oxylipins."}]},{"@id":"https://cir.nii.ac.jp/crid/1360298338407462656","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Sphingolipids with 2-hydroxy fatty acids aid in plasma membrane nanodomain organization and oxidative burst"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1104/pp.109.145094"},{"@type":"CROSSREF","@value":"10.1093/plphys/kiac134_references_DOI_DUQDmJrhHXiVvWhWTUO7zRmZdEr"},{"@type":"CROSSREF","@value":"10.1016/j.pbi.2015.05.019_references_DOI_DUQDmJrhHXiVvWhWTUO7zRmZdEr"}]}