{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362262946421600896.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1128/iai.00461-10"}},{"identifier":{"@type":"URI","@value":"https://journals.asm.org/doi/pdf/10.1128/IAI.00461-10"}}],"dc:title":[{"@value":"Development of Two Animal Models To Study the Function of\n            <i>Vibrio parahaemolyticus</i>\n            Type III Secretion Systems"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            <jats:italic>Vibrio parahaemolyticus</jats:italic>\n            is an emerging food- and waterborne pathogen that encodes two type III secretion systems (T3SSs). Previous studies have linked type III secretion system 1 (T3SS1) to cytotoxicity and T3SS2 to intestinal fluid accumulation, but animal challenge models needed to study these phenomena are limited. In this study we evaluated the roles of the T3SSs during infection using two novel animal models: a model in which piglets were inoculated orogastrically and a model in which mice were inoculated in their lungs (intrapulmonarily). The bacterial strains employed in this study had equivalent growth rates and beta-hemolytic activity based on\n            <jats:italic>in vitro</jats:italic>\n            assays. Inoculation of 48-h-old conventional piglets with 10\n            <jats:sup>11</jats:sup>\n            CFU of the wild-type strain (NY-4) or T3SS1 deletion mutant strains resulted in acute, self-limiting diarrhea, whereas inoculation with a T3SS2 deletion mutant strain failed to produce any clinical symptoms. Intrapulmonary inoculation of C57BL/6 mice with the wild-type strain and T3SS2 deletion mutant strains (5 × 10\n            <jats:sup>5</jats:sup>\n            CFU) induced mortality or a moribund state within 12 h (80 to 100% mortality), whereas inoculation with a T3SS1 deletion mutant or a T3SS1 T3SS2 double deletion mutant produced no mortality. Bacteria were recovered from multiple organs regardless of the strain used in the mouse model, indicating that the mice were capable of clearing the lung infection in the absence of a functional T3SS1. Because all strains had a similar beta-hemolysin phenotype, we surmise that thermostable direct hemolysin (TDH) plays a limited role in these models. The two models introduced herein produce robust results and provide a means to determine how different T3SS1 and T3SS2 effector proteins contribute to pathogenesis of\n            <jats:italic>V. parahaemolyticus</jats:italic>\n            infection.\n          </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382262946421600899","@type":"Researcher","foaf:name":[{"@value":"Pablo Piñeyro"}],"jpcoar:affiliationName":[{"@value":"Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262946421600898","@type":"Researcher","foaf:name":[{"@value":"Xiaohui Zhou"}],"jpcoar:affiliationName":[{"@value":"Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262946421600896","@type":"Researcher","foaf:name":[{"@value":"Lisa H. Orfe"}],"jpcoar:affiliationName":[{"@value":"Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262946421600900","@type":"Researcher","foaf:name":[{"@value":"Patrick J. Friel"}],"jpcoar:affiliationName":[{"@value":"Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262946421600897","@type":"Researcher","foaf:name":[{"@value":"Kevin Lahmers"}],"jpcoar:affiliationName":[{"@value":"Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040"}]},{"@id":"https://cir.nii.ac.jp/crid/1382262946421600901","@type":"Researcher","foaf:name":[{"@value":"Douglas R. Call"}],"jpcoar:affiliationName":[{"@value":"Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00199567"},{"@type":"EISSN","@value":"10985522"}],"prism:publicationName":[{"@value":"Infection and Immunity"}],"dc:publisher":[{"@value":"American Society for Microbiology"}],"prism:publicationDate":"2010-11","prism:volume":"78","prism:number":"11","prism:startingPage":"4551","prism:endingPage":"4559"},"reviewed":"false","dc:rights":["https://journals.asm.org/non-commercial-tdm-license"],"url":[{"@id":"https://journals.asm.org/doi/pdf/10.1128/IAI.00461-10"}],"createdAt":"2010-09-08","modifiedAt":"2022-03-05","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360285708449588864","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Export of a Vibrio parahaemolyticus toxin by the Sec and type III secretion machineries in tandem"}]},{"@id":"https://cir.nii.ac.jp/crid/1360564064219069824","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Advances on <i>Vibrio parahaemolyticus</i> research in the postgenomic era"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565169466009088","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Inflammation and Disintegration of Intestinal Villi in an Experimental Model for Vibrio parahaemolyticus-Induced Diarrhea"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567185450030336","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Regulation of<i>V</i><i>ibrio parahaemolyticus</i> T3SS2 gene expression and function of T3SS2 effectors that modulate actin cytoskeleton"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848660631380864","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Identification of the Vibrio parahaemolyticus type III secretion system 2-associated chaperone VocC for the T3SS2-specific effector VopC"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848661054111744","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"VopB1 and VopD1 are essential for translocation of type III secretion system 1 effectors of <i>Vibrio parahaemolyticus</i>"}]},{"@id":"https://cir.nii.ac.jp/crid/1360861705581832832","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The Xenogeneic Silencer Histone-Like Nucleoid-Structuring Protein Mediates the Temperature and Salinity-Dependent Regulation of the Type III Secretion System 2 in Vibrio parahaemolyticus"}]},{"@id":"https://cir.nii.ac.jp/crid/1390851010456573824","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Vibrio parahaemolyticus induces inflammation-associated fluid accumulation via activation of the cystic fibrosis transmembrane conductance regulator"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1128/iai.00461-10"},{"@type":"CROSSREF","@value":"10.1111/1348-0421.12767_references_DOI_V4VhJH5ZfaliSBqwgngO2YxXTR8"},{"@type":"CROSSREF","@value":"10.1038/s41564-019-0368-y_references_DOI_V4VhJH5ZfaliSBqwgngO2YxXTR8"},{"@type":"CROSSREF","@value":"10.2152/jmi.68.59_references_DOI_V4VhJH5ZfaliSBqwgngO2YxXTR8"},{"@type":"CROSSREF","@value":"10.1371/journal.ppat.1002593_references_DOI_V4VhJH5ZfaliSBqwgngO2YxXTR8"},{"@type":"CROSSREF","@value":"10.1111/cmi.12408_references_DOI_V4VhJH5ZfaliSBqwgngO2YxXTR8"},{"@type":"CROSSREF","@value":"10.1111/j.1574-6968.2011.02399.x_references_DOI_V4VhJH5ZfaliSBqwgngO2YxXTR8"},{"@type":"CROSSREF","@value":"10.1139/w2012-081_references_DOI_V4VhJH5ZfaliSBqwgngO2YxXTR8"},{"@type":"CROSSREF","@value":"10.1128/jb.00266-22_references_DOI_V4VhJH5ZfaliSBqwgngO2YxXTR8"}]}