{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362544418508640512.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1021/ja036211w"}},{"identifier":{"@type":"URI","@value":"https://pubs.acs.org/doi/pdf/10.1021/ja036211w"}},{"identifier":{"@type":"PMID","@value":"12926943"}}],"dc:title":[{"@value":"Direct Observation of Guanine Radical Cation Deprotonation in Duplex DNA Using Pulse Radiolysis"}],"description":[{"notation":[{"@value":"The dynamics of one-electron oxidation of guanine (G) base mononucleotide and that in DNA have been investigated by pulse radiolysis. The radical cation (G+*) of deoxyguanosine (dG), produced by oxidation with SO(4)-*, rapidly deprotonates to form the neutral G radical (G(-H)*) with a rate constant of 1.8 x 10(7) s(-1) at pH 7.0, as judged from transient spectroscopy. With experiments using different double-stranded oligonucleotides containing G, GG, and GGG sequences, the absorbance increases at 625 nm, characteristic of formation of the G(-H)*, were found to consist of two phases. The rate constants of the faster ( approximately 1.3 x 10(7) s(-1)) and slower phases ( approximately 3.0 x 10(6) s(-1)) were similar for the different oligonucleotides. On the other hand, in the oligonucleotide containing G located at the 5'- and 3'-terminal positions, only the faster phase was seen. These results suggest that the lifetime of the radical cation of the G:C base pair (GC+*), depending on its location in the DNA chain, is longer than that of free dG. In addition, the absorption spectral intermediates showed that hole transport to a specific G site within a 12-13mer double-stranded oligonucleotide is complete within 50 ns; that is, the rate of hole transport over 20 A is10(7) s(-1)."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382544418508640512","@type":"Researcher","foaf:name":[{"@value":"Kazuo Kobayashi"}],"jpcoar:affiliationName":[{"@value":"Contribution from The Institute of Scientific and Industrial Research, Osaka University, Mihogaoka 8-1, Ibaraki Osaka 567-0047, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1382544418508640513","@type":"Researcher","foaf:name":[{"@value":"Seiichi Tagawa"}],"jpcoar:affiliationName":[{"@value":"Contribution from The Institute of Scientific and Industrial Research, Osaka University, Mihogaoka 8-1, Ibaraki Osaka 567-0047, Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00027863"},{"@type":"EISSN","@value":"15205126"}],"prism:publicationName":[{"@value":"Journal of the American Chemical Society"}],"dc:publisher":[{"@value":"American Chemical Society (ACS)"}],"prism:publicationDate":"2003-08-01","prism:volume":"125","prism:number":"34","prism:startingPage":"10213","prism:endingPage":"10218"},"reviewed":"false","url":[{"@id":"https://pubs.acs.org/doi/pdf/10.1021/ja036211w"}],"createdAt":"2003-08-20","modifiedAt":"2021-10-09","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Guanine","dc:title":"Guanine"},{"@id":"https://cir.nii.ac.jp/all?q=Free%20Radicals","dc:title":"Free Radicals"},{"@id":"https://cir.nii.ac.jp/all?q=Oligonucleotides","dc:title":"Oligonucleotides"},{"@id":"https://cir.nii.ac.jp/all?q=DNA","dc:title":"DNA"},{"@id":"https://cir.nii.ac.jp/all?q=Cations","dc:title":"Cations"},{"@id":"https://cir.nii.ac.jp/all?q=Protons","dc:title":"Protons"},{"@id":"https://cir.nii.ac.jp/all?q=Pulse%20Radiolysis","dc:title":"Pulse Radiolysis"},{"@id":"https://cir.nii.ac.jp/all?q=Oxidation-Reduction","dc:title":"Oxidation-Reduction"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360002219105133184","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Formation of Pyrene Dimer Radical Cation at the Minor Groove of DNA"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004229997093376","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Proton Transfer Accompanied by the Oxidation of Adenosine"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283694073502976","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A B3LYP Study on Repair of Guanyl and 8-Oxoguanyl Radical by Simultaneous Proton- and Electron-Transfer Reaction"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285704895873280","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Hole Trapping of G‐Quartets in a G‐Quadruplex"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285708556938880","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Product analysis of photooxidation in isolated quadruplex DNA; 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