{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362544418924016384.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1152/ajpheart.2000.279.1.h285"}},{"identifier":{"@type":"URI","@value":"https://www.physiology.org/doi/pdf/10.1152/ajpheart.2000.279.1.H285"}},{"identifier":{"@type":"PMID","@value":"10899068"}}],"dc:title":[{"@value":"P2X<sub>4</sub> receptors mediate ATP-induced calcium influx  in human vascular endothelial cells"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p> ATP induces Ca<jats:sup>2+</jats:sup> influx across the cell membrane and activates release from intracellular Ca<jats:sup>2+</jats:sup> pools in vascular endothelial cells (ECs). Ca<jats:sup>2+</jats:sup> signaling leads to the modification of a variety of EC functions, including the production of vasoactive substances such as nitric oxide and prostacyclin. However, the molecular mechanisms for ATP-induced Ca<jats:sup>2+</jats:sup> influx in ECs have not been thoroughly clarified. Here we demonstrate evidence that a P2X<jats:sub>4</jats:sub>receptor for an ATP-gated cation channel is predominantly expressed in human ECs and is involved in the ATP-induced Ca<jats:sup>2+</jats:sup> influx. Northern blot analysis distinctly showed the expression of P2X<jats:sub>4</jats:sub> mRNA in human ECs cultured from the umbilical vein, aorta, pulmonary artery, and skin microvessels. Competitive PCR revealed that P2X<jats:sub>4</jats:sub> mRNA expression was much higher in ECs than was the expression of other subtypes, including P2X<jats:sub>1</jats:sub>, P2X<jats:sub>3</jats:sub>, P2X<jats:sub>5</jats:sub>, and P2X<jats:sub>7</jats:sub>. Treatment of ECs with antisense oligonucleotides designed to target the P2X<jats:sub>4</jats:sub> receptor decreased the P2X<jats:sub>4</jats:sub> mRNA and protein levels to ∼25% of control levels and markedly prevented the ATP-induced Ca<jats:sup>2+</jats:sup> influx. </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382544418924016388","@type":"Researcher","foaf:name":[{"@value":"Zhi Qi"}],"jpcoar:affiliationName":[{"@value":"Department of Physiology, Nagoya University School of Medicine, Nagoya 464-8601, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1382544418924016387","@type":"Researcher","foaf:name":[{"@value":"Masahiro Sokabe"}],"jpcoar:affiliationName":[{"@value":"Department of Physiology, Nagoya University School of Medicine, Nagoya 464-8601, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1382544418924016389","@type":"Researcher","foaf:name":[{"@value":"Joji Ando"}],"jpcoar:affiliationName":[{"@value":"Department of Biomedical Engineering, Graduate School of Medicine, University of Tokyo, Tokyo 113-0033; and"}]},{"@id":"https://cir.nii.ac.jp/crid/1382544418924016386","@type":"Researcher","foaf:name":[{"@value":"Akira Kamiya"}],"jpcoar:affiliationName":[{"@value":"Department of Biomedical Engineering, Graduate School of Medicine, University of Tokyo, Tokyo 113-0033; and"}]},{"@id":"https://cir.nii.ac.jp/crid/1382544418924016384","@type":"Researcher","foaf:name":[{"@value":"Kimiko Yamamoto"}],"jpcoar:affiliationName":[{"@value":"Department of Biomedical Engineering, Graduate School of Medicine, University of Tokyo, Tokyo 113-0033; and"}]},{"@id":"https://cir.nii.ac.jp/crid/1382544418924016385","@type":"Researcher","foaf:name":[{"@value":"Risa Korenaga"}],"jpcoar:affiliationName":[{"@value":"Department of Biomedical Engineering, Graduate School of Medicine, University of Tokyo, Tokyo 113-0033; and"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"03636135"},{"@type":"EISSN","@value":"15221539"},{"@type":"PISSN","@value":"https://id.crossref.org/issn/00029505"},{"@type":"PISSN","@value":"http://id.crossref.org/issn/00029505"}],"prism:publicationName":[{"@value":"American Journal of Physiology-Heart and Circulatory Physiology"}],"dc:publisher":[{"@value":"American Physiological Society"}],"prism:publicationDate":"2000-07-01","prism:volume":"279","prism:number":"1","prism:startingPage":"H285","prism:endingPage":"H292"},"reviewed":"false","url":[{"@id":"https://www.physiology.org/doi/pdf/10.1152/ajpheart.2000.279.1.H285"}],"createdAt":"2017-12-22","modifiedAt":"2019-09-08","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Umbilical%20Veins","dc:title":"Umbilical Veins"},{"@id":"https://cir.nii.ac.jp/all?q=Transcription,%20Genetic","dc:title":"Transcription, Genetic"},{"@id":"https://cir.nii.ac.jp/all?q=Blotting,%20Western","dc:title":"Blotting, Western"},{"@id":"https://cir.nii.ac.jp/all?q=Molecular%20Sequence%20Data","dc:title":"Molecular Sequence Data"},{"@id":"https://cir.nii.ac.jp/all?q=Pulmonary%20Artery","dc:title":"Pulmonary Artery"},{"@id":"https://cir.nii.ac.jp/all?q=Oligodeoxyribonucleotides,%20Antisense","dc:title":"Oligodeoxyribonucleotides, Antisense"},{"@id":"https://cir.nii.ac.jp/all?q=Adenosine%20Triphosphate","dc:title":"Adenosine Triphosphate"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Amino%20Acid%20Sequence","dc:title":"Amino Acid Sequence"},{"@id":"https://cir.nii.ac.jp/all?q=RNA,%20Messenger","dc:title":"RNA, Messenger"},{"@id":"https://cir.nii.ac.jp/all?q=Cloning,%20Molecular","dc:title":"Cloning, Molecular"},{"@id":"https://cir.nii.ac.jp/all?q=Egtazic%20Acid","dc:title":"Egtazic Acid"},{"@id":"https://cir.nii.ac.jp/all?q=Aorta","dc:title":"Aorta"},{"@id":"https://cir.nii.ac.jp/all?q=Cells,%20Cultured","dc:title":"Cells, Cultured"},{"@id":"https://cir.nii.ac.jp/all?q=Skin","dc:title":"Skin"},{"@id":"https://cir.nii.ac.jp/all?q=Receptors,%20Purinergic%20P2","dc:title":"Receptors, Purinergic P2"},{"@id":"https://cir.nii.ac.jp/all?q=Reverse%20Transcriptase%20Polymerase%20Chain%20Reaction","dc:title":"Reverse Transcriptase Polymerase Chain Reaction"},{"@id":"https://cir.nii.ac.jp/all?q=Microcirculation","dc:title":"Microcirculation"},{"@id":"https://cir.nii.ac.jp/all?q=Peptide%20Fragments","dc:title":"Peptide Fragments"},{"@id":"https://cir.nii.ac.jp/all?q=Receptors,%20Purinergic%20P2X","dc:title":"Receptors, Purinergic P2X"},{"@id":"https://cir.nii.ac.jp/all?q=Calcium","dc:title":"Calcium"},{"@id":"https://cir.nii.ac.jp/all?q=Endothelium,%20Vascular","dc:title":"Endothelium, Vascular"},{"@id":"https://cir.nii.ac.jp/all?q=Receptors,%20Purinergic%20P2X4","dc:title":"Receptors, Purinergic P2X4"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050022708918899456","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Mechanical homeostasis of liver sinusoid is involved in the initiation and termination of liver regeneration"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004236322382080","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Shear stress augments mitochondrial ATP generation that triggers ATP release and Ca<sup>2+</sup>signaling in vascular endothelial cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360290617808015744","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Shear stress activates mitochondrial oxidative phosphorylation by reducing plasma membrane cholesterol in vascular endothelial 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Endothelial Cells Occurring in Response to an Alternating Electric Stimulus"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204272066688","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Shear-stress sensing via P2 purinoceptors in vascular endothelial cells"},{"@language":"ja","@value":"血管内皮細胞のプリノセプターを介した流れずり応力感知機構"},{"@language":"ja-Kana","@value":"ケッカン ナイヒ サイボウ ノ プリノセプター オ カイシタ ナガレズリ オウリョク カンチ キコウ"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204272665600","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Extracellular ATP mediated mechano-signaling in mammary glands"},{"@language":"ja","@value":"乳腺における機械刺激とATP放出"},{"@language":"ja-Kana","@value":"ニュウセン ニ オケル キカイ シゲキ ト ATP 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