{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362544418935382016.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1007/s00438-010-0574-z"}},{"identifier":{"@type":"URI","@value":"http://link.springer.com/content/pdf/10.1007/s00438-010-0574-z.pdf"}},{"identifier":{"@type":"URI","@value":"http://link.springer.com/article/10.1007/s00438-010-0574-z/fulltext.html"}},{"identifier":{"@type":"URI","@value":"http://www.springerlink.com/index/pdf/10.1007/s00438-010-0574-z"}},{"identifier":{"@type":"PMID","@value":"20872230"}}],"dc:title":[{"@value":"Functional divergence within class B MADS-box genes TfGLO and TfDEF in Torenia fournieri Lind"}],"description":[{"notation":[{"@value":"Homeotic class B genes GLOBOSA (GLO)/PISTILLATA (PI) and DEFICIENS (DEF)/APETALA3 (AP3) are involved in the development of petals and stamens in Arabidopsis. However, functions of these genes in the development of floral organs in torenia are less well known. Here, we demonstrate the unique floral phenotypes of transgenic torenia formed due to the modification of class B genes, TfGLO and TfDEF. TfGLO-overexpressing plants showed purple-stained sepals that accumulated anthocyanins in a manner similar to that of petals. TfGLO-suppressed plants showed serrated petals and TfDEF-suppressed plants showed partially decolorized petals. In TfGLO-overexpressing plants, cell shapes on the surfaces of sepals were altered to petal-like cell shapes. Furthermore, TfGLO- and TfDEF-suppressed plants partially had sepal-like cells on the surfaces of their petals. We isolated putative class B gene-regulated genes and examined their expression in transgenic plants. Three xyloglucan endo-1,4-beta-D: -glucanase genes were up-regulated in TfGLO- and TfDEF-overexpressing plants and down-regulated in TfGLO- and TfDEF-suppressed plants. In addition, 10 anthocyanin biosynthesis-related genes, including anthocyanin synthase and chalcone isomerase, were up-regulated in TfGLO-overexpressing plants and down-regulated in TfGLO-suppressed plants. The expression patterns of these 10 genes in TfDEF transgenic plants were diverse and classified into several groups. HPLC analysis indicated that sepals of TfGLO-overexpressing plants accumulate the same type of anthocyanins and flavones as wild-type plants. The difference in phenotypes and expression patterns of the 10 anthocyanin biosynthesis-related genes between TfGLO and TfDEF transgenic plants indicated that TfGLO and TfDEF have partial functional divergence, while they basically work synergistically in torenia."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382544418935382018","@type":"Researcher","foaf:name":[{"@value":"Katsutomo Sasaki"}]},{"@id":"https://cir.nii.ac.jp/crid/1382544418935382019","@type":"Researcher","foaf:name":[{"@value":"Ryutaro Aida"}]},{"@id":"https://cir.nii.ac.jp/crid/1382544418935382016","@type":"Researcher","foaf:name":[{"@value":"Hiroyasu Yamaguchi"}]},{"@id":"https://cir.nii.ac.jp/crid/1382544418935382020","@type":"Researcher","foaf:name":[{"@value":"Masahito Shikata"}]},{"@id":"https://cir.nii.ac.jp/crid/1382544418935382022","@type":"Researcher","foaf:name":[{"@value":"Tomoya Niki"}]},{"@id":"https://cir.nii.ac.jp/crid/1382544418935382021","@type":"Researcher","foaf:name":[{"@value":"Takaaki Nishijima"}]},{"@id":"https://cir.nii.ac.jp/crid/1382544418935382017","@type":"Researcher","foaf:name":[{"@value":"Norihiro Ohtsubo"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"16174615"},{"@type":"EISSN","@value":"16174623"}],"prism:publicationName":[{"@value":"Molecular Genetics and Genomics"}],"dc:publisher":[{"@value":"Springer Science and Business Media LLC"}],"prism:publicationDate":"2010-09-26","prism:volume":"284","prism:number":"5","prism:startingPage":"399","prism:endingPage":"414"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["https://creativecommons.org/licenses/by-nc/2.0"],"url":[{"@id":"http://link.springer.com/content/pdf/10.1007/s00438-010-0574-z.pdf"},{"@id":"http://link.springer.com/article/10.1007/s00438-010-0574-z/fulltext.html"},{"@id":"http://www.springerlink.com/index/pdf/10.1007/s00438-010-0574-z"}],"createdAt":"2010-09-25","modifiedAt":"2021-11-10","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Homeodomain%20Proteins","dc:title":"Homeodomain Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Original%20Paper","dc:title":"Original Paper"},{"@id":"https://cir.nii.ac.jp/all?q=Genetic%20Variation","dc:title":"Genetic Variation"},{"@id":"https://cir.nii.ac.jp/all?q=Flavones","dc:title":"Flavones"},{"@id":"https://cir.nii.ac.jp/all?q=Plants,%20Genetically%20Modified","dc:title":"Plants, Genetically Modified"},{"@id":"https://cir.nii.ac.jp/all?q=DEFICIENS%20Protein","dc:title":"DEFICIENS Protein"},{"@id":"https://cir.nii.ac.jp/all?q=Anthocyanins","dc:title":"Anthocyanins"},{"@id":"https://cir.nii.ac.jp/all?q=Gene%20Expression%20Regulation,%20Plant","dc:title":"Gene Expression Regulation, Plant"},{"@id":"https://cir.nii.ac.jp/all?q=Genetics","dc:title":"Genetics"},{"@id":"https://cir.nii.ac.jp/all?q=Ferns","dc:title":"Ferns"},{"@id":"https://cir.nii.ac.jp/all?q=Microscopy,%20Electron,%20Scanning","dc:title":"Microscopy, Electron, Scanning"},{"@id":"https://cir.nii.ac.jp/all?q=Molecular%20Biology","dc:title":"Molecular Biology"},{"@id":"https://cir.nii.ac.jp/all?q=Phylogeny","dc:title":"Phylogeny"},{"@id":"https://cir.nii.ac.jp/all?q=Plant%20Proteins","dc:title":"Plant Proteins"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360285711757200256","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Molecular characterization of mutations in white-flowered torenia plants"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285711771731328","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Frontiers of torenia research: innovative ornamental traits and study of ecological interaction networks through genetic engineering"}]},{"@id":"https://cir.nii.ac.jp/crid/1361131421953829248","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A homolog of the ALOG family controls corolla tube differentiation in <i>Torenia 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Flowers"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001205289333376","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Role of Floral Homeotic Genes in the Morphology of Forchlorfenuron-induced Paracorollas in <i>Torenia fournieri</i> Lind."},{"@value":"ホルクロルフェニュロンにより誘導されるトレニアの副花冠の形態に対するホメオティック遺伝子の役割の解析"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679303486720","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Trehalose drastically extends the in vitro vegetative culture period and facilitates maintenance of Torenia fournieri plants"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679303490432","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Utilization of a floral organ-expressing AP1 promoter for generation of new floral traits in Torenia fournieri Lind"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679306103680","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Generation of fluorescent flowers exhibiting strong fluorescence by combination of fluorescent protein from marine plankton and recent genetic tools in <i>Torenia fournieri</i> Lind."},{"@value":"Generation of fluorescent flowers exhibiting strong fluorescence by combination of fluorescent protein from marine plankton and recent genetic tools in Torenia fournieri Lind"}]},{"@id":"https://cir.nii.ac.jp/crid/1390564238105274368","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Measuring plant 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