{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362544419001535360.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1007/bf00656929"}},{"identifier":{"@type":"URI","@value":"http://link.springer.com/content/pdf/10.1007/BF00656929.pdf"}},{"identifier":{"@type":"URI","@value":"http://link.springer.com/article/10.1007/BF00656929/fulltext.html"}},{"identifier":{"@type":"URI","@value":"http://link.springer.com/content/pdf/10.1007/BF00656929"}}],"dc:title":[{"@value":"Hydrogel microspheres III. Temperature-dependent adsorption of proteins on poly-N-isopropylacrylamide hydrogel microspheres"}],"description":[{"notation":[{"@value":"Precipitation polymerization of N-isopropylacrylamide (NIPAM) with methylenebisacrylamide (MBAAm) in water at 70°C gave thermosensitive hydrogel microspheres. The adsorbability of proteins on the poly-NIPAM microspheres was found to depend on temperature. Below the lower critical solution temperature (LCST) of poly-NIPAM in an aqueous medium, that is, around 32°C, the microspheres hold a large amount of water inside and their surface is hydrophilic enough to suppress the adsorption of proteins. On the contrary, above 32°C, the micropheres deswell and their surface becomes hydrophobic and, consequently, susceptible to adsorption of a large amount of proteins. Proteins once adsorbed on the microspheres at a high temperature could be desorbed more or less by lowering the temperature to below 32°C. The extent of desorption at low temperatures was found to depend on the incubation time for adsorption at high temperatures."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382544419001535361","@type":"Researcher","foaf:name":[{"@value":"Haruma Kawaguchi"}]},{"@id":"https://cir.nii.ac.jp/crid/1420564276188081024","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"70229045"},{"@type":"NRID","@value":"1000070229045"},{"@type":"NRID","@value":"9000018384552"},{"@type":"NRID","@value":"9000394464705"},{"@type":"NRID","@value":"9000254399904"},{"@type":"NRID","@value":"9000002915762"},{"@type":"NRID","@value":"9000283144807"},{"@type":"NRID","@value":"9000254933497"},{"@type":"NRID","@value":"9000257749579"},{"@type":"NRID","@value":"9000000470930"},{"@type":"NRID","@value":"9000242255014"},{"@type":"NRID","@value":"9000258615170"},{"@type":"NRID","@value":"9000007050025"},{"@type":"NRID","@value":"9000412277035"},{"@type":"NRID","@value":"9000258685129"},{"@type":"NRID","@value":"9000408458715"},{"@type":"NRID","@value":"9000017355213"},{"@type":"NRID","@value":"9000410177595"},{"@type":"NRID","@value":"9000254933502"},{"@type":"NRID","@value":"9000360547315"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0050679"}],"foaf:name":[{"@value":"Keiji Fujimoto"}]},{"@id":"https://cir.nii.ac.jp/crid/1382544419001535362","@type":"Researcher","foaf:name":[{"@value":"Yoshiro Mizuhara"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"0303402X"},{"@type":"EISSN","@value":"14351536"},{"@type":"PISSN","@value":"http://id.crossref.org/issn/0303402X"}],"prism:publicationName":[{"@value":"Colloid & Polymer Science"}],"dc:publisher":[{"@value":"Springer Science and Business Media LLC"}],"prism:publicationDate":"1992-01","prism:volume":"270","prism:number":"1","prism:startingPage":"53","prism:endingPage":"57"},"reviewed":"false","dc:rights":["http://www.springer.com/tdm"],"url":[{"@id":"http://link.springer.com/content/pdf/10.1007/BF00656929.pdf"},{"@id":"http://link.springer.com/article/10.1007/BF00656929/fulltext.html"},{"@id":"http://link.springer.com/content/pdf/10.1007/BF00656929"}],"createdAt":"2004-11-28","modifiedAt":"2019-04-05","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004233456811520","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Direct visualization of swollen microgels by scanning electron microscopy using ionic liquids"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283691587921152","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Investigation of Changes in the Microscopic Structure of Anionic Poly(<i>N</i>-isopropylacrylamide-<i>co</i>-Acrylic acid) Microgels in the Presence of Cationic Organic Dyes toward Precisely Controlled Uptake/Release of Low-Molecular-Weight Chemical Compound"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285707520803584","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Sono-respond on thermosensitive polymer microgels based on cross-linked poly(N-isopropylacrylamide-co-acrylic acid)"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285708433376000","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Interaction between synthetic particles and biomacromolecules: fundamental study of nonspecific interaction and design of nanoparticles that recognize target molecules"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565169064289408","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Sonoresponsive Volume Phase-transition Behavior of Crosslinked Poly(<i>N</i>-isopropylacrylamide-<i>co</i>-acrylic acid) Microgel"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567182023585664","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Production of nanofibers by atto-incubator-assisted assembly of urea using the particle array"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204092167680","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Micro Hydrogels: Preparation, Properties, and Applications"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204139036544","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"ナノテクノロジー講座（第ＩＩＩ講）ミクロゲル"},{"@language":"en","@value":"Micro-gel"},{"@value":"ナノテクノロジー講座(第3講)ミクロゲル"},{"@language":"ja-Kana","@value":"ナノテクノロジー コウザ ダイ3コウ ミクロゲル"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204565600384","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Adsorption of Triton X-100, Tryptophan and BSA on Temperature-Sensitive Poly(Vinylmethylether)Gel."}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204566228736","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Temperature-Swing Column Adsorption of Nonionic Surfactant with Poly(vinylmethylether) Gel."},{"@value":"Temperature-Swing Column Adsorption of Nonionic Surfactant with Poly(vinylmethylether) Gel(SC)"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001206290172928","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Stimuli-Response of Microsphere Having Poly(N-isopropylacrylamide) Shell."}]},{"@id":"https://cir.nii.ac.jp/crid/1390001206290652160","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Thermosensitive Behavior of Poly(N-isopropylacrylamide) Grafted Polystyrene Nanoparticles"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679486069376","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"反応性界面活性剤を利用した感温性ゲル微粒子の合成"},{"@language":"en","@value":"Synthesis of Thermosensitive Microgel Particles by Using Polymerizable Surfactant"},{"@value":"材料工学,デバイス 反応性界面活性剤を利用した感温性ゲル微粒子の合成"},{"@language":"ja-Kana","@value":"ザイリョウ コウガク デバイス ハンノウセイ カイメン カッセイザイ オ リヨウ シタ カンオンセイ ゲル ビリュウシ ノ ゴウセイ"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282680446583936","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"総論 (ポリマー微粒子)"},{"@language":"en","@value":"Polymeric Microspheres"},{"@value":"微粒子応用講座(第2講)総論(ポリマー微粒子)"},{"@language":"ja-Kana","@value":"ビリュウシ オウヨウ コウザ ダイ 2 コウ ソウロン ポリマー ビリュウシ"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282681503433344","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Preparation and Characterization of Micrometer-sized Poly(N-isopropylacrylamide) Hydrogel Particles: Effect of Initiator Concentration"},{"@language":"ja","@value":"ミクロンサイズのポリ（Ｎ－イソプロピルアクリルアミド）ヒドロゲル粒子の作製：開始剤の濃度と種類の影響"}]},{"@id":"https://cir.nii.ac.jp/crid/1390846609812419456","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Protein Adsorption to 2D-arrayed microgels on SPR chip"}]},{"@id":"https://cir.nii.ac.jp/crid/1521417754771371648","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Preparation of Thermo-Sensitive Magnetic Hydrogel Microspheres and Application to Enzyme Immobilization"},{"@language":"ja-Kana","@value":"Preparation of Thermo-Sensitive Magneti"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1007/bf00656929"},{"@type":"OPENAIRE","@value":"doi_dedup___::9a10e1b0fab4b7fcba9459cea15df8d6"},{"@type":"CROSSREF","@value":"10.1295/polymj.31.1139_references_DOI_HCMP23I2rOKVE4aX7A2uqLin7EC"},{"@type":"CROSSREF","@value":"10.1038/pj.2015.103_references_DOI_HCMP23I2rOKVE4aX7A2uqLin7EC"},{"@type":"CROSSREF","@value":"10.4011/shikizai.82.264_references_DOI_HCMP23I2rOKVE4aX7A2uqLin7EC"},{"@type":"CROSSREF","@value":"10.1295/polymj.35.901_references_DOI_HCMP23I2rOKVE4aX7A2uqLin7EC"},{"@type":"CROSSREF","@value":"10.1038/pj.2014.33_references_DOI_HCMP23I2rOKVE4aX7A2uqLin7EC"},{"@type":"CROSSREF","@value":"10.1016/s0922-338x(97)89255-0_references_DOI_HCMP23I2rOKVE4aX7A2uqLin7EC"},{"@type":"CROSSREF","@value":"10.1252/jcej.34.1317_references_DOI_HCMP23I2rOKVE4aX7A2uqLin7EC"},{"@type":"CROSSREF","@value":"10.1246/cl.2006.776_references_DOI_HCMP23I2rOKVE4aX7A2uqLin7EC"},{"@type":"CROSSREF","@value":"10.1252/jcej.28.697_references_DOI_HCMP23I2rOKVE4aX7A2uqLin7EC"},{"@type":"CROSSREF","@value":"10.1021/acs.langmuir.6b00760_references_DOI_HCMP23I2rOKVE4aX7A2uqLin7EC"},{"@type":"CROSSREF","@value":"10.4011/shikizai1937.71.272_references_DOI_HCMP23I2rOKVE4aX7A2uqLin7EC"},{"@type":"CROSSREF","@value":"10.1252/kakoronbunshu.29.400_references_DOI_HCMP23I2rOKVE4aX7A2uqLin7EC"},{"@type":"CROSSREF","@value":"10.1295/koron.65.751_references_DOI_HCMP23I2rOKVE4aX7A2uqLin7EC"},{"@type":"CROSSREF","@value":"10.5650/jos.62.865_references_DOI_HCMP23I2rOKVE4aX7A2uqLin7EC"},{"@type":"CROSSREF","@value":"10.1016/j.colsurfa.2012.02.036_references_DOI_HCMP23I2rOKVE4aX7A2uqLin7EC"},{"@type":"CROSSREF","@value":"10.1016/j.ultsonch.2013.02.003_references_DOI_HCMP23I2rOKVE4aX7A2uqLin7EC"},{"@type":"CROSSREF","@value":"10.14723/tmrsj.33.359_references_DOI_HCMP23I2rOKVE4aX7A2uqLin7EC"}]}