{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362544420157702272.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/j.phytochem.2010.02.008"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S003194221000052X?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S003194221000052X?httpAccept=text/plain"}},{"identifier":{"@type":"PMID","@value":"20223486"}}],"dc:title":[{"@value":"Co-pigmentation and flavonoid glycosyltransferases in blue Veronica persica flowers"}],"description":[{"notation":[{"@value":"Glycosylation is one of the key modification steps for plants to produce a broad spectrum of flavonoids with various structures and colors. A survey of flavonoids in the blue flowers of Veronica persica Poiret (Lamiales, Scrophulariaceae), which is native of Eurasia and now widespread worldwide, led to the identification of highly glycosylated flavonoids, namely delphinidin 3-O-(2-O-(6-O-p-coumaroyl-glucosyl)-6-O-p-coumaroyl-glucoside)-5-O-glucoside (1) and apigenin 7-O-(2-O-glucuronosyl)-glucuronide (2), as two of its main flavonoids. Interestingly, the latter flavone glucuronide (2) caused a bathochromic shift on the anthocyanin (1) toward a blue hue in a dose-dependent manner, showing an intermolecular co-pigment effect. In order to understand the molecular basis for the biosynthesis of this glucuronide, we isolated a cDNA encoding a UDP-dependent glycosyltransferase (UGT88D8), based on the structural similarity to flavonoid 7-O-glucuronosyltransferases (F7GAT) from Lamiales plants. Enzyme assays showed that the recombinant UGT88D8 protein catalyzes the 7-O-glucuronosylation of apigenin and its related flavonoids with preference to UDP-glucuronic acid as a sugar donor. Furthermore, we identified and functionally characterized a cDNA encoding another UGT, UGT94F1, as the anthocyanin 3-O-glucoside-2''-O-glucosyltransferase (A3Glc2''GlcT), according to the structural similarity to sugar-sugar glycosyltransferases classified to the cluster IV of flavonoid UGTs. Preferential expression of UGT88D8 and UGT94F1 genes in the petals supports the idea that these UGTs play an important role in the biosynthesis of key flavonoids responsible for the development of the blue color of V. persica flowers."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382544420157702276","@type":"Researcher","foaf:name":[{"@value":"Eiichiro Ono"}]},{"@id":"https://cir.nii.ac.jp/crid/1382544420157702274","@type":"Researcher","foaf:name":[{"@value":"Miho Ruike"}]},{"@id":"https://cir.nii.ac.jp/crid/1382544420157702272","@type":"Researcher","foaf:name":[{"@value":"Takashi Iwashita"}]},{"@id":"https://cir.nii.ac.jp/crid/1382544420157702273","@type":"Researcher","foaf:name":[{"@value":"Kyosuke Nomoto"}]},{"@id":"https://cir.nii.ac.jp/crid/1382544420157702275","@type":"Researcher","foaf:name":[{"@value":"Yuko Fukui"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00319422"}],"prism:publicationName":[{"@value":"Phytochemistry"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"2010-05","prism:volume":"71","prism:number":"7","prism:startingPage":"726","prism:endingPage":"735"},"reviewed":"false","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S003194221000052X?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S003194221000052X?httpAccept=text/plain"}],"createdAt":"2010-03-11","modifiedAt":"2023-05-30","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Flavonoids","dc:title":"Flavonoids"},{"@id":"https://cir.nii.ac.jp/all?q=Spectrometry,%20Mass,%20Electrospray%20Ionization","dc:title":"Spectrometry, Mass, Electrospray Ionization"},{"@id":"https://cir.nii.ac.jp/all?q=Magnetic%20Resonance%20Spectroscopy","dc:title":"Magnetic Resonance Spectroscopy"},{"@id":"https://cir.nii.ac.jp/all?q=Base%20Sequence","dc:title":"Base Sequence"},{"@id":"https://cir.nii.ac.jp/all?q=Molecular%20Sequence%20Data","dc:title":"Molecular Sequence Data"},{"@id":"https://cir.nii.ac.jp/all?q=Glycosyltransferases","dc:title":"Glycosyltransferases"},{"@id":"https://cir.nii.ac.jp/all?q=Flowers","dc:title":"Flowers"},{"@id":"https://cir.nii.ac.jp/all?q=Pigments,%20Biological","dc:title":"Pigments, Biological"},{"@id":"https://cir.nii.ac.jp/all?q=Hydrogen-Ion%20Concentration","dc:title":"Hydrogen-Ion Concentration"},{"@id":"https://cir.nii.ac.jp/all?q=Veronica","dc:title":"Veronica"},{"@id":"https://cir.nii.ac.jp/all?q=Cloning,%20Molecular","dc:title":"Cloning, Molecular"},{"@id":"https://cir.nii.ac.jp/all?q=Phylogeny","dc:title":"Phylogeny"},{"@id":"https://cir.nii.ac.jp/all?q=DNA%20Primers","dc:title":"DNA Primers"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360002217848176128","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Functional Differentiation of the Glycosyltransferases That Contribute to the Chemical Diversity of Bioactive Flavonol Glycosides in Grapevines (<i>Vitis vinifera</i>)"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285709620346624","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Identification of the glucosyltransferase gene that supplies the p-hydroxybenzoyl-glucose for 7-polyacylation of anthocyanin in delphinium"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137045142505600","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Glycoside‐specific glycosyltransferases catalyze regio‐selective sequential glucosylations for a sesame lignan, sesaminol triglucoside"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204326607488","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Transcriptional regulators of flavonoid biosynthesis and their application to flower color modification in Japanese gentians"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001205759547392","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"タイワンヤマツツジ（<i>Rhododendron simsii</i> Planch.）の花弁ブロッチにおけるアントシアニン–フラボノールコピグメンテーション"},{"@language":"en","@value":"Co-pigmentation of Anthocyanin-flavonol in the Blotch Area of <i>Rhododendron simsii</i> Planch. Flowers"},{"@value":"Co-pigmentation of Anthocyanin-flavonol in the Blotch Area of Rhododendron simsii Planch. Flowers"}]},{"@id":"https://cir.nii.ac.jp/crid/1390005506388073088","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Characterization of Ornamental Plants in <i>Veronica ornata</i> Monjuschko Native to Oki Islands, Japan"},{"@language":"ja","@value":"隠岐諸島に自生するトウテイラン（<i>Veronica ornata</i> Monjuschko）の園芸化を目的とした各種形質の評価"},{"@value":"隠岐諸島に自生するトウテイラン(Veronica ornata Monjuschko)の園芸化を目的とした各種形質の評価"},{"@language":"ja-Kana","@value":"オキ ショトウ ニ ジセイ スル トウテイラン(Veronica ornata Monjuschko)ノ エンゲイカ オ モクテキ ト シタ カクシュ ケイシツ ノ ヒョウカ"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1016/j.phytochem.2010.02.008"},{"@type":"OPENAIRE","@value":"doi_dedup___::5faa1c5919cd19c29735bb329efd0953"},{"@type":"CROSSREF","@value":"10.5511/plantbiotechnology.14.0731a_references_DOI_W6f22ZuYqwXl5HfeULcPwiSD21i"},{"@type":"CROSSREF","@value":"10.1093/jxb/eru134_references_DOI_W6f22ZuYqwXl5HfeULcPwiSD21i"},{"@type":"CROSSREF","@value":"10.1105/tpc.110.074625_references_DOI_W6f22ZuYqwXl5HfeULcPwiSD21i"},{"@type":"CROSSREF","@value":"10.1111/tpj.14586_references_DOI_W6f22ZuYqwXl5HfeULcPwiSD21i"},{"@type":"CROSSREF","@value":"10.2503/hrj.19.339_references_DOI_W6f22ZuYqwXl5HfeULcPwiSD21i"},{"@type":"CROSSREF","@value":"10.2503/hortj.mi-092_references_DOI_W6f22ZuYqwXl5HfeULcPwiSD21i"}]}