{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362544420231672576.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1530/jme-18-0078"}},{"identifier":{"@type":"URI","@value":"https://jme.bioscientifica.com/view/journals/jme/61/4/JME-18-0078.xml"}},{"identifier":{"@type":"URI","@value":"https://syndication.highwire.org/content/doi/10.1530/JME-18-0078"}}],"dc:title":[{"@value":"ADSC-conditioned media elicit an ex vivo anti-inflammatory macrophage response"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>Obesity-associated inflammatory mechanisms play a key role in the pathogenesis of metabolic-related diseases. Failure of anti-inflammatory control mechanisms within adipose tissue and peripheral blood mononuclear cells (PBMCs) have been implicated in disease progression. This study investigated the efficacy of allogeneic adipose tissue-derived mesenchymal stem cells conditioned media (ADSC-CM) to counteract persistent inflammation by inducing an anti-inflammatory phenotype and cytokine response within PBMCs derived from patients with and without metabolic syndrome. Forty-six (<jats:italic>n</jats:italic> = 46) mixed ancestry females (18–45 years) were subdivided into (a) healthy lean (HL) (<jats:italic>n</jats:italic> = 10) (BMI <25 kg/m<jats:sup>2</jats:sup>), (b) overweight/obese (OW/OB) (BMI ≥25 kg/m<jats:sup>2</jats:sup>, <3 metabolic risk factors) (<jats:italic>n</jats:italic> = 22) and (c) metabolic syndrome (MetS) (visceral adiposity, ≥3 metabolic risk factors) (<jats:italic>n</jats:italic> = 14) groups. Body composition (DXA scan), metabolic (cholesterol, HDL, LDL, triglycerides, blood glucose) and inflammatory profiles (38-Plex cytokine panel) were determined. PBMCs were isolated from whole blood and treated <jats:italic>ex vivo</jats:italic> with either (i) autologous participant-derived serum, (ii) ADSCs-CM or (iii) a successive treatment regime. The activation status (CD11b+) and intracellular cytokine (IL6, IL10, TNFa) expression were determined in M1 (CD68+CD206−CD163−) and M2 (CD68+CD163+ CD206+) macrophage populations using flow cytometry. ADSC-CM treatment, promoted a M2 macrophage phenotype and induced IL10 expression, this was most pronounced in the OW/OB group. This response is likely mediated by multiple complementing factors within ADSC-CM, yet to be identified. This study is the first to demonstrate the therapeutic potential of ADSC-CM to restore the inflammatory balance in immune compromised obese individuals.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382544420231672577","@type":"Researcher","foaf:name":[{"@value":"Maria Jacoba Kruger"}],"jpcoar:affiliationName":[{"@value":"Division of Endocrinology, Department of Medicine, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa"}]},{"@id":"https://cir.nii.ac.jp/crid/1382544420231672578","@type":"Researcher","foaf:name":[{"@value":"Maria Martha Conradie"}],"jpcoar:affiliationName":[{"@value":"Division of Endocrinology, Department of Medicine, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa"}]},{"@id":"https://cir.nii.ac.jp/crid/1382544420231672579","@type":"Researcher","foaf:name":[{"@value":"Magda Conradie"}],"jpcoar:affiliationName":[{"@value":"Division of Endocrinology, Department of Medicine, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa"}]},{"@id":"https://cir.nii.ac.jp/crid/1382544420231672576","@type":"Researcher","foaf:name":[{"@value":"Mari van de Vyver"}],"jpcoar:affiliationName":[{"@value":"Division of Endocrinology, Department of Medicine, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"09525041"},{"@type":"EISSN","@value":"14796813"}],"prism:publicationName":[{"@value":"Journal of Molecular Endocrinology"}],"dc:publisher":[{"@value":"Bioscientifica"}],"prism:publicationDate":"2018-11","prism:volume":"61","prism:number":"4","prism:startingPage":"173","prism:endingPage":"184"},"reviewed":"false","url":[{"@id":"https://jme.bioscientifica.com/view/journals/jme/61/4/JME-18-0078.xml"},{"@id":"https://syndication.highwire.org/content/doi/10.1530/JME-18-0078"}],"createdAt":"2018-07-23","modifiedAt":"2020-07-27","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360580229795223552","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Effects of conditioned medium obtained from human adipose-derived stem cells on skin inflammation"}]},{"@id":"https://cir.nii.ac.jp/crid/1390285300151790208","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Effects of Adipose Tissue-Derived Stem Cells Transplanted to a Bone Defect after Irradiation"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1530/jme-18-0078"},{"@type":"CROSSREF","@value":"10.2485/jhtb.29.111_references_DOI_CxdY9yMBsJiToodLP9zCCJrtgIN"},{"@type":"CROSSREF","@value":"10.1016/j.reth.2022.03.009_references_DOI_CxdY9yMBsJiToodLP9zCCJrtgIN"}]}