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Determination of Carcinoembryonic Antigen in Human Sera by Integrated Bead-Bed Immunoasay in a Microchip for Cancer Diagnosis
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- Manabu Tokeshi
- Department of Applied Chemistry, School of Engineering, The University of Tokyo, Hongo, Bunkyo, Tokyo 113-8656, Japan, Integrated Chemistry Project, Kanagawa Academy of Science and Technology, Sakado, Takatsu, Kawasaki, Kanagawa 213-0021, Japan, and Department of Forensic Medicine, School of Medicine, Juntendo University, Hongo, Bunkyo, Tokyo 113-8421, Japan
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- Hiroko Kimura
- Department of Applied Chemistry, School of Engineering, The University of Tokyo, Hongo, Bunkyo, Tokyo 113-8656, Japan, Integrated Chemistry Project, Kanagawa Academy of Science and Technology, Sakado, Takatsu, Kawasaki, Kanagawa 213-0021, Japan, and Department of Forensic Medicine, School of Medicine, Juntendo University, Hongo, Bunkyo, Tokyo 113-8421, Japan
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- Kiichi Sato
- Department of Applied Chemistry, School of Engineering, The University of Tokyo, Hongo, Bunkyo, Tokyo 113-8656, Japan, Integrated Chemistry Project, Kanagawa Academy of Science and Technology, Sakado, Takatsu, Kawasaki, Kanagawa 213-0021, Japan, and Department of Forensic Medicine, School of Medicine, Juntendo University, Hongo, Bunkyo, Tokyo 113-8421, Japan
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- Takehiko Kitamori
- Department of Applied Chemistry, School of Engineering, The University of Tokyo, Hongo, Bunkyo, Tokyo 113-8656, Japan, Integrated Chemistry Project, Kanagawa Academy of Science and Technology, Sakado, Takatsu, Kawasaki, Kanagawa 213-0021, Japan, and Department of Forensic Medicine, School of Medicine, Juntendo University, Hongo, Bunkyo, Tokyo 113-8421, Japan
Bibliographic Information
- Published
- 2001-02-10
- DOI
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- 10.1021/ac000991z
- Publisher
- American Chemical Society (ACS)
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Description
A bead-bed immunoassay system was structured on a microchip and applied to determine carcinoembryonic antigen (CEA), which is a commonly used marker of colon cancer. Polystyrene beads precoated with anti-CEA antibody were introduced into a microchannel, and then a serum sample containing CEA, the first antibody, and the second antibody conjugated with colloidal gold were reacted successively. The resulting antigen-antibodies complex, fixed on the bead surface, was detected using a thermal lens microscope (TLM). A highly selective and sensitive determination of an ultratrace amount of CEA in human sera was made possible by a sandwich immunoassay system that needs three antibodies for an assay. A detection limit dozens of times lower than the conventional ELISA was achieved. Moreover, when serum samples for 13 patients were assayed with this system, there was a high correlation (r = 0.917) with the conventional ELISA. The integration reduced the time necessary for the antigen-antibody reaction to approximately 1%, thus shortening the overall analysis time from 45 h to 35 min. Moreover, troublesome operations required for conventional heterogeneous immunoassays could be much simplified. This microchip-based diagnosis system is the first microchip-based system that is practically useful for clinical diagnoses with short analysis time, high sensitivity, and easy procedures.
Journal
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- Analytical Chemistry
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Analytical Chemistry 73 (6), 1213-1218, 2001-02-10
American Chemical Society (ACS)
