{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362825893936822144.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1021/acsomega.9b01323"}},{"identifier":{"@type":"URI","@value":"http://pubs.acs.org/doi/pdf/10.1021/acsomega.9b01323"}},{"identifier":{"@type":"URI","@value":"https://pubs.acs.org/doi/pdf/10.1021/acsomega.9b01323"}},{"identifier":{"@type":"PMID","@value":"31460251"}}],"dc:title":[{"@value":"Plasmid DNA Mono-Ion Complex for in Vivo Sustainable Gene Expression"}],"description":[{"notation":[{"@value":"To cleave biocompatible poly(ethylene glycol) (PEG) from the mono-ion complex (MIC) for sustainable cellular uptake in vivo, ω-amide-pentylimidazolium end-modified PEG with an ester bond, that is, APe-Im-E-PEG, has been synthesized. The hydrolysis of the resulting APe-Im-E-PEG proceeded during the incubation for 2 weeks under physiological conditions, which was confirmed by gel filtration chromatography. APe-Im-E-PEG formed the MIC with plasmid DNA (pDNA), assessed by agarose gel retardation assay. Furthermore, dynamic light scattering measurement and transmission electron microscopy observations have estimated that the particle size of the resulting MIC was approximately 30 nm, with a rather flexible structure. The APe-Im-E-PEG/pDNA MIC incubated for 2 weeks exhibited hemolytic activity at endosomal pH, presumably because the pH-sensitive carboxyl groups revealed after the hydrolysis of an ester bond of APe-Im-E-PEG. The APe-Im-E-PEG/pDNA MIC enhanced the gene expression 2 weeks after transfection in vivo by intramuscular administration in mice. Consequently, in vivo sustainable gene expression has been achieved by the molecular design of APe-Im-E-PEG for cellular uptake and endosomal escape proceeded by temporal hydrolysis of the ester bond."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382825893936822145","@type":"Researcher","foaf:name":[{"@value":"Yuki Kobayashi"}],"jpcoar:affiliationName":[{"@value":"Department of Applied Chemistry, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo 192-0397, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825893936822147","@type":"Researcher","foaf:name":[{"@value":"Sakura Taneichi"}],"jpcoar:affiliationName":[{"@value":"Department of Applied Chemistry, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo 192-0397, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825893936822144","@type":"Researcher","foaf:name":[{"@value":"Hiroyoshi Kawakami"}],"jpcoar:affiliationName":[{"@value":"Department of Applied Chemistry, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo 192-0397, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825893936822146","@type":"Researcher","foaf:name":[{"@value":"Yoichi Negishi"}],"jpcoar:affiliationName":[{"@value":"Department of Drug Delivery and Molecular Biopharmaceutics, Tokyo University of Pharmacy and Life Sciences, Horinouchi, Hachioji, Tokyo 192-0392, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825893936822148","@type":"Researcher","foaf:name":[{"@value":"Shoichiro Asayama"}],"jpcoar:affiliationName":[{"@value":"Department of Applied Chemistry, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo 192-0397, Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"24701343"},{"@type":"EISSN","@value":"24701343"}],"prism:publicationName":[{"@value":"ACS Omega"}],"dc:publisher":[{"@value":"American Chemical Society (ACS)"}],"prism:publicationDate":"2019-07-01","prism:volume":"4","prism:number":"7","prism:startingPage":"11464","prism:endingPage":"11471"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["http://pubs.acs.org/page/policy/authorchoice_termsofuse.html"],"url":[{"@id":"http://pubs.acs.org/doi/pdf/10.1021/acsomega.9b01323"},{"@id":"https://pubs.acs.org/doi/pdf/10.1021/acsomega.9b01323"}],"createdAt":"2019-07-01","modifiedAt":"2023-04-26","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Chemistry","dc:title":"Chemistry"},{"@id":"https://cir.nii.ac.jp/all?q=QD1-999","dc:title":"QD1-999"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360306905630617984","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Synthesis of Diethylamino End‐Modified Poly(Ethylene Glycol) With Imine Spacer to Form Mono‐Ion Complexes With <scp>pDNA</scp>"}]},{"@id":"https://cir.nii.ac.jp/crid/1360579814639740032","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Tunable Gene Expression in Skeletal Muscles by the Molecular Weight of PEG Chain Length of Plasmid DNA Mono-ion Complexes"}]},{"@id":"https://cir.nii.ac.jp/crid/1360857593802457344","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Guanidinopropyl end‐modified poly(ethylene glycol) to form highly compact plasmid <scp>DNA</scp> mono‐ion complexes by thermal treatment"}]},{"@id":"https://cir.nii.ac.jp/crid/2051151842055755904","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Molecular design of polymer-based carriers for plasmid DNA delivery in vitro and in vivo"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1021/acsomega.9b01323"},{"@type":"OPENAIRE","@value":"doi_dedup___::186bd11b458cb07c77b990ac94313fe0"},{"@type":"CROSSREF","@value":"10.1002/pat.70025_references_DOI_2kYFfDNMvyYje3GwUyruLa3gIPf"},{"@type":"CROSSREF","@value":"10.1246/cl.220242_references_DOI_2kYFfDNMvyYje3GwUyruLa3gIPf"},{"@type":"CROSSREF","@value":"10.1246/cl.190696_references_DOI_2kYFfDNMvyYje3GwUyruLa3gIPf"},{"@type":"CROSSREF","@value":"10.1002/pat.5530_references_DOI_2kYFfDNMvyYje3GwUyruLa3gIPf"}]}