{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362825894054786304.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1126/science.1076979"}},{"identifier":{"@type":"URI","@value":"https://www.science.org/doi/pdf/10.1126/science.1076979"}}],"dc:title":[{"@value":"Rewiring MAP Kinase Pathways Using Alternative Scaffold Assembly Mechanisms"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>How scaffold proteins control information flow in signaling pathways is poorly understood: Do they simply tether components, or do they precisely orient and activate them? We found that the yeast mitogen-activated protein (MAP) kinase scaffold Ste5 is tolerant to major stereochemical perturbations; heterologous protein interactions could functionally replace native kinase recruitment interactions, indicating that simple tethering is largely sufficient for scaffold-mediated signaling. Moreover, by engineering a scaffold that tethers a unique kinase set, we could create a synthetic MAP kinase pathway with non-natural input-output properties. These findings demonstrate that scaffolds are highly flexible organizing factors that can facilitate pathway evolution and engineering.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382825894054786304","@type":"Researcher","foaf:name":[{"@value":"Sang-Hyun Park"}],"jpcoar:affiliationName":[{"@value":"Department of Cellular and Molecular Pharmacology and Department of Biochemistry and Biophysics, University of California, 513 Parnassus Avenue, San Francisco, CA 94143, USA."}]},{"@id":"https://cir.nii.ac.jp/crid/1382825894054786306","@type":"Researcher","foaf:name":[{"@value":"Ali Zarrinpar"}],"jpcoar:affiliationName":[{"@value":"Department of Cellular and Molecular Pharmacology and Department of Biochemistry and Biophysics, University of California, 513 Parnassus Avenue, San Francisco, CA 94143, USA."}]},{"@id":"https://cir.nii.ac.jp/crid/1382825894054786305","@type":"Researcher","foaf:name":[{"@value":"Wendell A. Lim"}],"jpcoar:affiliationName":[{"@value":"Department of Cellular and Molecular Pharmacology and Department of Biochemistry and Biophysics, University of California, 513 Parnassus Avenue, San Francisco, CA 94143, USA."}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00368075"},{"@type":"EISSN","@value":"10959203"}],"prism:publicationName":[{"@value":"Science"}],"dc:publisher":[{"@value":"American Association for the Advancement of Science (AAAS)"}],"prism:publicationDate":"2003-02-14","prism:volume":"299","prism:number":"5609","prism:startingPage":"1061","prism:endingPage":"1064"},"reviewed":"false","url":[{"@id":"https://www.science.org/doi/pdf/10.1126/science.1076979"}],"createdAt":"2003-02-13","modifiedAt":"2024-01-09","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360285706909416960","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"RNA and RNP as Building Blocks for Nanotechnology and Synthetic Biology"}]},{"@id":"https://cir.nii.ac.jp/crid/1360306904393967488","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Chemical genetics reveals cross-regulation of plant developmental signaling by the immune peptide-receptor pathway"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565164414028672","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Nucleic‐Acid‐Templated Enzyme Cascades"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565169653101568","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Response to Hyperosmotic Stress"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848656862837888","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"RNA Study Using DNA Nanotechnology"}]},{"@id":"https://cir.nii.ac.jp/crid/1360857593798530560","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Reprogramming signal transduction through a designer receptor tyrosine kinase"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001206129173888","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"酵母を用いた合成生物学 ～新しい人工生命システム構築への挑戦～"},{"@language":"en","@value":"Synthetic Biology Using Yeast ∼Challenge for Creation of Novel Artificial Life Systems∼"},{"@language":"ja-Kana","@value":"コウボ オ モチイタ ゴウセイ セイブツガク : アタラシイ ジンコウ セイメイ システム コウチク エ ノ チョウセン"},{"@value":"Synthetic Biology Using Yeast &sim;Challenge for Creation of Novel Artificial Life Systems&sim;"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1126/science.1076979"},{"@type":"CROSSREF","@value":"10.1016/bs.pmbts.2015.12.004_references_DOI_WfTjdwTSQ2IjXrRqlQEvdG39RTR"},{"@type":"CROSSREF","@value":"10.1126/sciadv.ads3718_references_DOI_WfTjdwTSQ2IjXrRqlQEvdG39RTR"},{"@type":"CROSSREF","@value":"10.1002/cbic.201600703_references_DOI_WfTjdwTSQ2IjXrRqlQEvdG39RTR"},{"@type":"CROSSREF","@value":"10.1534/genetics.112.140863_references_DOI_WfTjdwTSQ2IjXrRqlQEvdG39RTR"},{"@type":"CROSSREF","@value":"10.6013/jbrewsocjapan.106.728_references_DOI_WfTjdwTSQ2IjXrRqlQEvdG39RTR"},{"@type":"CROSSREF","@value":"10.1038/s42003-021-02287-8_references_DOI_WfTjdwTSQ2IjXrRqlQEvdG39RTR"},{"@type":"CROSSREF","@value":"10.1016/bs.pmbts.2015.11.004_references_DOI_WfTjdwTSQ2IjXrRqlQEvdG39RTR"}]}