{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362825894172677888.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.2164/jandrol.107.002725"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.2164%2Fjandrol.107.002725"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/pdf/10.2164/jandrol.107.002725"}}],"dc:title":[{"@value":"Cryosurvival and In Vitro Fertilizing Capacity Postthaw Is Improved When Boar Spermatozoa Are Frozen in the Presence of Seminal Plasma From Good Freezer Boars"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p><jats:bold>ABSTRACT: </jats:bold> The study evaluated the protective effect of seminal plasma (SP) added to freezing extender against cryopreservation injuries to boar spermatozoa. Pooled sperm‐rich fractions collected from 9 fertile boars were frozen in 0.5‐mL straws after being extended in a conventional freezing extender either alone or supplemented with 5% of SPs (SP1‐SP4) collected from the sperm‐rich fractions (diluted 1:1, vol/vol, in Beltsville Thawing Solution extender) from 4 boars (1–4) with known sperm cryosurvival (poor, moderate, and good sperm freezers). Cryopreservation injuries were assessed in terms of postthaw sperm motility (assessed by computer‐assisted sperm analysis), viability (plasma membrane and acrosome integrity assessed simultaneously by flow cytometry), membrane lipid peroxidation (malondialdehyde [MDA] production), and the ability of thawed spermatozoa to fertilize in vitro—matured homologous oocytes. The addition of SP from good sperm freezers (SP3 and SP4) improved (<jats:italic>P</jats:italic> < .01) the motility and viability of thawed spermatozoa without any influence on MDA production. Moreover, SP from good sperm freezers also increased (<jats:italic>P</jats:italic> < .05) the percentage of penetrated (SP3) and polyspermic oocytes (SP4) with respect to the control. Neither the total amount of SP proteins, protein profiles, nor antioxidant capacity of the different SPs were related to the various cryosurvival/fertilizing capacities of the processed spermatozoa.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380861294055940480","@type":"Researcher","foaf:name":[{"@value":"Marta Hernández"}]},{"@id":"https://cir.nii.ac.jp/crid/1380861294055940487","@type":"Researcher","foaf:name":[{"@value":"Jordi Roca"}]},{"@id":"https://cir.nii.ac.jp/crid/1380861294055940484","@type":"Researcher","foaf:name":[{"@value":"Juan J. Calvete"}]},{"@id":"https://cir.nii.ac.jp/crid/1380861294055940483","@type":"Researcher","foaf:name":[{"@value":"Libia Sanz"}]},{"@id":"https://cir.nii.ac.jp/crid/1380861294055940486","@type":"Researcher","foaf:name":[{"@value":"Teresa Muiño‐Blanco"}]},{"@id":"https://cir.nii.ac.jp/crid/1380861294055940481","@type":"Researcher","foaf:name":[{"@value":"José A. Cebrián‐Pérez"}]},{"@id":"https://cir.nii.ac.jp/crid/1380861294055940482","@type":"Researcher","foaf:name":[{"@value":"Juan M. Vázquez"}]},{"@id":"https://cir.nii.ac.jp/crid/1380861294055940485","@type":"Researcher","foaf:name":[{"@value":"Emilio A. Martínez"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"01963635"},{"@type":"EISSN","@value":"19394640"}],"prism:publicationName":[{"@value":"Journal of Andrology"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2007-09-10","prism:volume":"28","prism:number":"5","prism:startingPage":"689","prism:endingPage":"697"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.2164%2Fjandrol.107.002725"},{"@id":"https://onlinelibrary.wiley.com/doi/pdf/10.2164/jandrol.107.002725"}],"createdAt":"2007-08-21","modifiedAt":"2023-10-17","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1390001206336233600","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"The Vanguard Sperm Cohort of the Boar Ejaculate is Overrepresented in the Tubal Sperm Reservoir In Vivo"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679686021504","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Effects of Ejaculated Fractions and Seminal Plasma onSperm Motility Following Freezing and Thawingin Native Ryukyu-Agu Pigs"},{"@language":"ja","@value":"琉球在来ブタアグーの射出精液分画と精漿が精子凍結融解後の運動性に及ぼす影響"},{"@language":"ja-Kana","@value":"リュウキュウ ザイライ ブタアグー ノ シャシュツ セイエキ ブンカク ト セイショウ ガ セイシ トウケツ ユウカイ ゴ ノ ウンドウセイ ニ オヨボス エイキョウ"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282681313598208","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"The Positive Effects of Seminal Plasma During the Freezing Process on Cryosurvival of Sperm with Poor Freezability in the Rhesus Macaque (Macaca mulatta)"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.2164/jandrol.107.002725"},{"@type":"CROSSREF","@value":"10.1262/jrd.11-056n_references_DOI_T5V4va8qvta0fMtB9eol8wLGrJG"},{"@type":"CROSSREF","@value":"10.1262/jrd.09-125k_references_DOI_T5V4va8qvta0fMtB9eol8wLGrJG"},{"@type":"CROSSREF","@value":"10.5938/youton.50.37_references_DOI_T5V4va8qvta0fMtB9eol8wLGrJG"}]}