{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362825894306425856.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1074/jbc.274.26.18201"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0021925819871466?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0021925819871466?httpAccept=text/plain"}},{"identifier":{"@type":"URI","@value":"https://syndication.highwire.org/content/doi/10.1074/jbc.274.26.18201"}},{"identifier":{"@type":"PMID","@value":"10373420"}},{"identifier":{"@type":"NAID","@value":"80011137723"}}],"dc:title":[{"@value":"The Oxidized Forms of dATP Are Substrates for the Human MutT Homologue, the hMTH1 Protein"}],"description":[{"notation":[{"@value":"The possibility that Escherichia coli MutT and human MTH1 (hMTH1) hydrolyze oxidized DNA precursors other than 8-hydroxy-dGTP (8-OH-dGTP) was investigated. We report here that hMTH1 hydrolyzed 2-hydroxy-dATP (2-OH-dATP) and 8-hydroxy-dATP (8-OH-dATP), oxidized forms of dATP, but not (R)-8,5'-cyclo-dATP, 5-hydroxy-dCTP, and 5-formyl-dUTP. The kinetic parameters indicated that 2-OH-dATP was hydrolyzed more efficiently and with higher affinity than 8-OH-dGTP. 8-OH-dATP was hydrolyzed as efficiently as 8-OH-dGTP. The preferential hydrolysis of 2-OH-dATP over 8-OH-dGTP was observed at all of the pH values tested (pH 7.2 to pH 8.8). In particular, a 5-fold difference in the hydrolysis efficiencies for 2-OH-dATP over 8-OH-dGTP was found at pH 7.2. However, E. coli MutT had no hydrolysis activity for either 2-OH-dATP or 8-OH-dATP. Thus, E. coli MutT is an imperfect counterpart for hMTH1. Furthermore, we found that 2-hydroxy-dADP and 8-hydroxy-dGDP competitively inhibited both the 2-OH-dATP hydrolase and 8-OH-dGTP hydrolase activities of hMTH1. The inhibitory effects of 2-hydroxy-dADP were 3-fold stronger than those of 8-hydroxy-dGDP. These results suggest that the three damaged nucleotides share the same recognition site of hMTH1 and that it is a more important sanitization enzyme than expected thus far."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382825894306425986","@type":"Researcher","foaf:name":[{"@value":"Katsuyoshi Fujikawa"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825894306425857","@type":"Researcher","foaf:name":[{"@value":"Hiroyuki Kamiya"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825894306425984","@type":"Researcher","foaf:name":[{"@value":"Hiroyuki Yakushiji"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825894306425858","@type":"Researcher","foaf:name":[{"@value":"Yoshimitsu Fujii"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825894306425856","@type":"Researcher","foaf:name":[{"@value":"Yusaku Nakabeppu"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825894306425985","@type":"Researcher","foaf:name":[{"@value":"Hiroshi Kasai"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00219258"}],"prism:publicationName":[{"@value":"Journal of Biological Chemistry"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"1999-06","prism:volume":"274","prism:number":"26","prism:startingPage":"18201","prism:endingPage":"18205"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/","http://creativecommons.org/licenses/by/4.0/"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S0021925819871466?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S0021925819871466?httpAccept=text/plain"},{"@id":"https://syndication.highwire.org/content/doi/10.1074/jbc.274.26.18201"}],"createdAt":"2002-07-26","modifiedAt":"2022-01-04","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Escherichia%20coli%20Proteins","dc:title":"Escherichia coli Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Hydrogen-Ion%20Concentration","dc:title":"Hydrogen-Ion Concentration"},{"@id":"https://cir.nii.ac.jp/all?q=Phosphoric%20Monoester%20Hydrolases","dc:title":"Phosphoric Monoester Hydrolases"},{"@id":"https://cir.nii.ac.jp/all?q=Substrate%20Specificity","dc:title":"Substrate Specificity"},{"@id":"https://cir.nii.ac.jp/all?q=Kinetics","dc:title":"Kinetics"},{"@id":"https://cir.nii.ac.jp/all?q=DNA%20Repair%20Enzymes","dc:title":"DNA Repair Enzymes"},{"@id":"https://cir.nii.ac.jp/all?q=Deoxyadenine%20Nucleotides","dc:title":"Deoxyadenine Nucleotides"},{"@id":"https://cir.nii.ac.jp/all?q=Bacterial%20Proteins","dc:title":"Bacterial Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Escherichia%20coli","dc:title":"Escherichia coli"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Pyrophosphatases","dc:title":"Pyrophosphatases"},{"@id":"https://cir.nii.ac.jp/all?q=Oxidation-Reduction","dc:title":"Oxidation-Reduction"},{"@id":"https://cir.nii.ac.jp/all?q=Chromatography,%20High%20Pressure%20Liquid","dc:title":"Chromatography, High Pressure Liquid"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050298532705408128","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"8-oxoguanine causes spontaneous de novo germline mutations in mice."}]},{"@id":"https://cir.nii.ac.jp/crid/1360004233979023744","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Human MTH3 (NUDT18) Protein Hydrolyzes Oxidized Forms of Guanosine and Deoxyguanosine Diphosphates"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004233982996992","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Structural and Kinetic Studies of the Human Nudix Hydrolase MTH1 Reveal the Mechanism for Its Broad Substrate Specificity"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285709646451200","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Diverse substrate recognition and hydrolysis mechanisms of human NUDT5"}]},{"@id":"https://cir.nii.ac.jp/crid/1360576118764003456","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The ALK inhibitors, alectinib and ceritinib, induce ALK‐independent and STAT3‐dependent glioblastoma cell death"}]},{"@id":"https://cir.nii.ac.jp/crid/1360580232406706432","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Protonation states of Asp residues in the human Nudix hydrolase\n                    <scp>MTH1</scp>\n                    contribute to its broad substrate recognition"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848660015309184","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Crystallization and preliminary X-ray analysis of human MTH1 with a homogeneous N-terminus"}]},{"@id":"https://cir.nii.ac.jp/crid/1360861704781459072","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"RAD51 Expression as a Biomarker to Predict Efficacy of Preoperative Therapy and Survival for Esophageal Squamous Cell Carcinoma"}]},{"@id":"https://cir.nii.ac.jp/crid/1360861705558156032","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Implications of N7-hydrogen and C8-keto on the base pairing, mutagenic potential and repair of 8-oxo-2′-deoxy-adenosine: Investigation by nucleotide analogues"}]},{"@id":"https://cir.nii.ac.jp/crid/1360869854345150336","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Visualization of oxidized guanine nucleotides accumulation in living cells with split MutT"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204147302912","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Molecular devices for high fidelity of DNA replication and gene expression"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001205700478592","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"レドックス制御に関わるアスコルビン酸およびヌクレオシドニリン酸類縁体(Nudix)"},{"@language":"en","@value":"Function of L-Ascorbate and Nucleoside Diphosphate Derivatives Involved in The Regulation of Redox Status in Higher Plants"},{"@language":"ja-Kana","@value":"レドックス セイギョ ニ カカワル アスコルビンサン オヨビ ヌクレオシドニリンサン ルイエンタイ Nudix"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001206153698432","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"DNA損傷の誘発する変異の分子機構"},{"@language":"en","@value":"Molecular Mechanisms of Mutations Induced by DNA Lesions"},{"@language":"ja-Kana","@value":"DNA ソンショウ ノ ユウハツ スル ヘンイ ノ ブンシ キコウ"},{"@value":"ChemInform Abstract: Molecular Mechanisms of Mutations Induced by DNA Lesions"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679174999296","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@value":"活性酸素による核酸の酸化損傷とその防御機構"},{"@language":"en","@value":"Oxidative Damage of Nucleic Acids Caused by Reactive Oxygen Species and Defense Mechanisms Against the Damage"},{"@language":"ja-Kana","@value":"カイセツ カッセイ サンソ ニ ヨル カクサン ノ サンカ ソンショウ ト ソノ ボウギョ キコウ"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679396356480","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"酸化的ＤＮＡ損傷誘発突然変異を回避する分子機構―ＤＮＡ修復欠損マウスにおける突然変異と発がんの解析―"},{"@language":"en","@value":"Spontaneous tumorigenesis and mutagenesis in DNA repair-deficient mice"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679397361408","@type":"Article","relationType":["isReferencedBy","isCitedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"ＤＮＡ前駆体の酸化損傷と突然変異"},{"@language":"en","@value":"Oxidative damage in nucleotide pool and mutagenesis"},{"@language":"ja-Kana","@value":"DNA ゼンクタイ ノ サンカ ソンショウ ト トツゼン ヘンイ"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679604188928","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy","isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Effects of Overexpression and Antisense RNA Expression of Orf17, a MutT-Type Enzyme"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679942086144","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Increased A: T→C: G Mutations in the <i>mutT</i> Strain upon 8-Hydroxy-dGTP Treatment: Direct Evidence for MutT Involvement in the Prevention of Mutations by Oxidized dGTP"},{"@value":"Increased A:T→C:G Mutations in the mutT Strain upon 8-Hydroxy-dGTP Treatment: Direct Evidence for MutT Involvement in the Prevention of Mutations by Oxidized dGTP"},{"@language":"ja-Kana","@value":"Increased A T C G Mutations in the mutT Strain upon 8 Hydroxy dGTP Treatment Direct Evidence for MutT Involvement in the Prevention of Mutations by Oxidized dGTP"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282680192110976","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy","isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"2-Hydroxyadenine in DNA is a Very Poor Substrate of the <i>Escherichia coli</i> MutY Protein"},{"@value":"2-Hydroxyadenine in DNA is a Very Poor Substrate of the Escherichia coli MutY Protein"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282680232852352","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy","isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Mutations Induced by Oxidized DNA Precursors and Their Prevention by Nucleotide Pool Sanitization Enzymes"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282680450725888","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy","isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"CiMutT, an asidian MutT homologue, has a 7, 8-dihydro-8-oxo-dGTP pyrophosphohydrolase activity responsible for sanitization of oxidized nucleotides in Ciona intestinalis"}]},{"@id":"https://cir.nii.ac.jp/crid/1390565134847369088","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Enzymatic characteristics of Nudix hydrolase 2 (Nud2), an 8-oxo-dGTP hydrolase from <i>Myxococcus xanthus</i>"}]},{"@id":"https://cir.nii.ac.jp/crid/1520009408038317184","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@value":"第115回 日本眼科学会総会 特別講演 網膜の包括的神経保護 : 臨床応用への挑戦"},{"@language":"ja-Kana","@value":"ダイ115カイ ニホン ガンカ ガッカイ ソウカイ トクベツ コウエン モウマク ノ ホウカツテキ シンケイ ホゴ : リンショウ オウヨウ エ ノ チョウセン"},{"@language":"ja","@value":"網膜の包括的神経保護 : 臨床応用への挑戦"}]},{"@id":"https://cir.nii.ac.jp/crid/1520013474483656192","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"nudt7 gene depletion causes transcriptomic change in early development of zebrafish"}]},{"@id":"https://cir.nii.ac.jp/crid/1520290882787207680","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@value":"酸化的DNA損傷誘発突然変異を回避する分子機構--DNA修復欠損マウスにおける突然変異と発がんの解析"},{"@language":"ja-Kana","@value":"サンカテキ DNA ソンショウ ユウハツ トツゼン ヘンイ オ カイヒ スル ブンシ キコウ DNA シュウフク ケッソン マウス ニ オケル トツゼン ヘンイ ト ハツガン ノ カイセキ"}]},{"@id":"https://cir.nii.ac.jp/crid/1520572357042642432","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@value":"損傷DNA前駆体の誘発変異とヌクレオチドプール浄化酵素/DNA修復酵素による抑制"},{"@language":"ja-Kana","@value":"ソンショウ DNA ゼンクタイ ノ ユウハツ ヘンイ ト ヌクレオチド プール ジョウカ コウソ DNA シュウフク コウソ ニ ヨル ヨクセイ"}]},{"@id":"https://cir.nii.ac.jp/crid/1520572358367440256","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@value":"活性酸素による突然変異誘発機構--大腸菌の鉄の取り込み調節機構とその活性酸素防御としての役割"},{"@language":"ja-Kana","@value":"カッセイ サンソ ニ ヨル トツゼン ヘンイ ユウハツ キコウ ダイチョウキン ノ テツ ノ トリコミ チョウセツ キコウ ト ソノ カッセイ サンソ ボウギョ ト シテ ノ ヤクワリ"},{"@language":"ja","@value":"活性酸素による突然変異誘発機構 : 大腸菌の鉄の取り込み調節機能とその活性酸素防御としての役割 : 第12回公開シンポジウム : 活性酸素の分子病態学"},{"@language":"en","@value":"Oxidative mutagenesis : Regulation of iron transporting systems and their role in protection from oxidative stress in Escherichia coli"}]},{"@id":"https://cir.nii.ac.jp/crid/1520853834383081088","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@value":"Mechanisms protecting genomic integrity from damage caused by reactive oxygen species: Implications for carcinogenesis and neurodegeneration"},{"@language":"ja-Kana","@value":"Mechanisms protecting genomic integrity from damage caused by reactive oxygen species Implications for carcinogenesis and neurodegeneration"}]},{"@id":"https://cir.nii.ac.jp/crid/1523951030563122176","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@value":"Recognition of nucleotide analogs containing the 7,8-dihydro-8-oxo structure by the human MTH1 protein"},{"@language":"ja-Kana","@value":"Recognition of nucleotide analogs containing the 7 8 dihydro 8 oxo structure by the human MTH1 protein"}]},{"@id":"https://cir.nii.ac.jp/crid/1571698600346371200","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"In vivo mutagenicity and initiation following oxidative DNA lesion in the kidneys of rats given potassium bromate"}]},{"@id":"https://cir.nii.ac.jp/crid/1571980074828415744","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Defense mechanism to oxidative DNA damage in glial cells"}]},{"@id":"https://cir.nii.ac.jp/crid/2051151841912727552","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Mutations induced by 8-hydroxyguanine (8-oxo-7,8-dihydroguanine), a representative oxidized base, in mammalian cells"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1074/jbc.274.26.18201"},{"@type":"CIA","@value":"80011137723"},{"@type":"OPENAIRE","@value":"doi_dedup___::553c59d9eaf195f2f90a34ebc8d67263"},{"@type":"CROSSREF","@value":"10.1269/jrr.41.349_references_DOI_VxMqiPnQtHIzTCEGXIvMGmtFcKX"},{"@type":"CROSSREF","@value":"10.1074/jbc.m112.363010_references_DOI_VxMqiPnQtHIzTCEGXIvMGmtFcKX"},{"@type":"CROSSREF","@value":"10.1074/jbc.m116.749713_references_DOI_VxMqiPnQtHIzTCEGXIvMGmtFcKX"},{"@type":"CROSSREF","@value":"10.3123/jems.26.159_references_DOI_VxMqiPnQtHIzTCEGXIvMGmtFcKX"},{"@type":"CROSSREF","@value":"10.1093/jb/mvac086_references_DOI_VxMqiPnQtHIzTCEGXIvMGmtFcKX"},{"@type":"CROSSREF","@value":"10.1093/nar/gkr575_references_DOI_VxMqiPnQtHIzTCEGXIvMGmtFcKX"},{"@type":"CROSSREF","@value":"10.3123/jems.27.101_references_DOI_VxMqiPnQtHIzTCEGXIvMGmtFcKX"},{"@type":"CROSSREF","@value":"10.1038/srep04689_references_DOI_VxMqiPnQtHIzTCEGXIvMGmtFcKX"},{"@type":"CROSSREF","@value":"10.3123/jemsge.29.133_references_DOI_VxMqiPnQtHIzTCEGXIvMGmtFcKX"},{"@type":"CROSSREF","@value":"10.2183/pjab.82.278_references_DOI_VxMqiPnQtHIzTCEGXIvMGmtFcKX"},{"@type":"CROSSREF","@value":"10.1111/cas.14885_references_DOI_VxMqiPnQtHIzTCEGXIvMGmtFcKX"},{"@type":"CROSSREF","@value":"10.1002/1873-3468.14611_references_DOI_VxMqiPnQtHIzTCEGXIvMGmtFcKX"},{"@type":"CROSSREF","@value":"10.1186/s41021-016-0051-y_references_DOI_VxMqiPnQtHIzTCEGXIvMGmtFcKX"},{"@type":"CROSSREF","@value":"10.1107/s1744309112048002_references_DOI_VxMqiPnQtHIzTCEGXIvMGmtFcKX"},{"@type":"CROSSREF","@value":"10.1266/ggs.85.287_references_DOI_VxMqiPnQtHIzTCEGXIvMGmtFcKX"},{"@type":"CROSSREF","@value":"10.1097/sla.0000000000003975_references_DOI_VxMqiPnQtHIzTCEGXIvMGmtFcKX"},{"@type":"CROSSREF","@value":"10.1016/j.bioorg.2022.106029_references_DOI_VxMqiPnQtHIzTCEGXIvMGmtFcKX"},{"@type":"CROSSREF","@value":"10.1093/nar/gkae371_references_DOI_VxMqiPnQtHIzTCEGXIvMGmtFcKX"},{"@type":"CROSSREF","@value":"10.2323/jgam.2019.04.002_references_DOI_VxMqiPnQtHIzTCEGXIvMGmtFcKX"},{"@type":"CROSSREF","@value":"10.1248/bpb.29.1087_references_DOI_VxMqiPnQtHIzTCEGXIvMGmtFcKX"}]}