{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362825894435003264.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1002/ijc.2910480515"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fijc.2910480515"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/ijc.2910480515"}},{"identifier":{"@type":"PMID","@value":"2071232"}}],"dc:title":[{"@value":"Establishment from a human chondrosarcoma of a new immortal cell line with high tumorigenicity <i>in vivo</i>, which is able to form proteoglycan‐rich cartilage‐like nodules and to respond to insulin <i>in vitro</i>"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title><jats:p>The human chondrosarcoma cell line (HCS‐2/8) established by our group expresses cartilage phenotypes such as production of cartilage‐type proteoglycans and collagen type II, but its tumorigenicity is low. To develop an <jats:italic>in vitro</jats:italic> experimental system for studies of human chondrosarcomas, a new immortal cell line of human chondrosarcoma, named HCS‐2/A, was established from the same tumor. HCS‐2/A cells proliferated with a doubling time of 3° days in a medium containing 20% fetal bovine serum (FBS). This growth rate was comparable to that of HCS‐2/8 cells. However, HCS‐2/A cells proliferated more rapidly than HCS‐2/8 cells in the presence of 2–10% FBS. Like HCS‐2/8 cells, HCS‐2/A cells had a polygonal shape in sparse cultures and became spherical as they reached confluence, after which they formed nodules composed of multi‐layered cells and a large quantity of extracellular matrix showing strong metachromasia. The nodules formed by HCS‐2/A cells were thicker and also larger in diameter than those formed by HCS‐2/8 cells. Electron microscopically, the cells in the nodules resembled chondrocytes <jats:italic>in vivo</jats:italic>, but each cell had an irregular‐shaped nucleus which is a characteristic of tumor cells. The cells actively synthesized “cartilage‐specific” large proteoglycans and their level of proteoglycan synthesis was comparable to that of HCS‐2/8 cells. Insulin, which stimulates proteoglycan and DNA syntheses in cultured chondrocytes, markedly Increased proteoglycan synthesis in HCS‐2/A cells. On the other hand, the hormone only slightly increased proteoglycan synthesis in HCS‐2/8 cells. Insulin also stimulated DNA synthesis in cultured HCS‐2/A cells, but not in HCS‐2/8 cells. Immunostaining revealed that HCS‐2/A cells produced type‐11 collagen but not type‐1 collagen. However, the level of collagen synthesis of HCS‐2/A cells was lower than that of HCS‐2/8 cells. Inoculation of HCS‐2/A cells into athymic mice resulted in the formation of chondrosarcomas that grew faster than those arising from HCS‐2/8 cells.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382825894435003265","@type":"Researcher","foaf:name":[{"@value":"Masaharu Takigawa"}]},{"@id":"https://cir.nii.ac.jp/crid/1380016867931594752","@type":"Researcher","foaf:name":[{"@value":"Hai‐Ou Pan"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825894435003267","@type":"Researcher","foaf:name":[{"@value":"Akihiro Kinoshita"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825894435003268","@type":"Researcher","foaf:name":[{"@value":"Koji Tajima"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825894435003266","@type":"Researcher","foaf:name":[{"@value":"Yoshiro Takano"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00207136"},{"@type":"EISSN","@value":"10970215"}],"prism:publicationName":[{"@value":"International Journal of Cancer"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"1991-07-09","prism:volume":"48","prism:number":"5","prism:startingPage":"717","prism:endingPage":"725"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fijc.2910480515"},{"@id":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/ijc.2910480515"}],"createdAt":"2007-02-19","modifiedAt":"2023-10-23","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=DNA%20Replication","dc:title":"DNA Replication"},{"@id":"https://cir.nii.ac.jp/all?q=Male","dc:title":"Male"},{"@id":"https://cir.nii.ac.jp/all?q=Sulfates","dc:title":"Sulfates"},{"@id":"https://cir.nii.ac.jp/all?q=Transplantation,%20Heterologous","dc:title":"Transplantation, Heterologous"},{"@id":"https://cir.nii.ac.jp/all?q=Chondrosarcoma","dc:title":"Chondrosarcoma"},{"@id":"https://cir.nii.ac.jp/all?q=Mice,%20Nude","dc:title":"Mice, Nude"},{"@id":"https://cir.nii.ac.jp/all?q=Bone%20Neoplasms","dc:title":"Bone Neoplasms"},{"@id":"https://cir.nii.ac.jp/all?q=Sulfur%20Radioisotopes","dc:title":"Sulfur Radioisotopes"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Line","dc:title":"Cell Line"},{"@id":"https://cir.nii.ac.jp/all?q=Clone%20Cells","dc:title":"Clone Cells"},{"@id":"https://cir.nii.ac.jp/all?q=Extracellular%20Matrix","dc:title":"Extracellular Matrix"},{"@id":"https://cir.nii.ac.jp/all?q=Kinetics","dc:title":"Kinetics"},{"@id":"https://cir.nii.ac.jp/all?q=Mice","dc:title":"Mice"},{"@id":"https://cir.nii.ac.jp/all?q=Microscopy,%20Electron","dc:title":"Microscopy, Electron"},{"@id":"https://cir.nii.ac.jp/all?q=Cartilage","dc:title":"Cartilage"},{"@id":"https://cir.nii.ac.jp/all?q=Culture%20Techniques","dc:title":"Culture Techniques"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Insulin","dc:title":"Insulin"},{"@id":"https://cir.nii.ac.jp/all?q=Proteoglycans","dc:title":"Proteoglycans"},{"@id":"https://cir.nii.ac.jp/all?q=Collagen","dc:title":"Collagen"},{"@id":"https://cir.nii.ac.jp/all?q=Neoplasm%20Transplantation","dc:title":"Neoplasm Transplantation"},{"@id":"https://cir.nii.ac.jp/all?q=Aged","dc:title":"Aged"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004231513021952","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"An early history of CCN2/CTGF research: the road to CCN2 via hcs24, ctgf, ecogenin, and regenerin"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283689554096768","@type":"Article","resourceType":"学術雑誌論文(journal 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