{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362825894601216000.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1562/2006-08-19-ir-1006"}},{"identifier":{"@type":"PMID","@value":"17132067"}}],"dc:title":[{"@value":"Sub-5-fs Real-time Spectroscopy of Transition States in Bacteriorhodopsin During Retinal Isomerization†"}],"dcterms:alternative":[{"@value":"Photochemistry and Photobiology, 2007, 83"}],"description":[{"notation":[{"@value":"By using a sub-5-fs visible laser pulse, we have made the first observation of the vibrational spectra of the transition state during trans-cis isomerization in the retinal chromophore of bacteriorhodopsin (bR(S68). No instant isomerization of the retinal occurs in spite of electron promotion from the bonding pi-orbital to the anti-bonding pi*-orbital. The difference between the in-plane and out-of-plane vibrational frequencies (about 1150-1250 and 900-1000 cm(-1), respectively) is reduced during the first time period. The vibrational spectra after this period became very broad and weak and are ascribed to a \"silent state.\" The silent state lasts for 700-900 fs until the chromophore isomerizes to the cis-C13 = C14 conformation. The frequency of the C = C stretching mode was modulated by the torsion mode of the C13 = C14 double bond with a period of 200 fs. The modulation was clearly observed for four to five periods. Using the empirical equation for the relation between bond length and stretching frequency, we determined the transitional C = C bond length with about 0.01 angstroms accuracy during the torsion motion around the double bond with 1-fs time resolution."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382825894601216000","@type":"Researcher","foaf:name":[{"@value":"Takayoshi Kobayashi"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825894601215873","@type":"Researcher","foaf:name":[{"@value":"Atsushi Yabushita"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825894601215872","@type":"Researcher","foaf:name":[{"@value":"Takashi Saito"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825894601216001","@type":"Researcher","foaf:name":[{"@value":"Hiroyuki Ohtani"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825894601216002","@type":"Researcher","foaf:name":[{"@value":"Motoyuki Tsuda"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00318655"}],"prism:publicationName":[{"@value":"Photochemistry and Photobiology"}],"dc:publisher":[{"@value":"American Society for Photobiology"}],"prism:publicationDate":"2007-03-28","prism:volume":"83","prism:number":"2","prism:startingPage":"363","prism:endingPage":"369"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","createdAt":"2010-09-21","modifiedAt":"2019-03-05","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Photochemistry","dc:title":"Photochemistry"},{"@id":"https://cir.nii.ac.jp/all?q=Spectrophotometry","dc:title":"Spectrophotometry"},{"@id":"https://cir.nii.ac.jp/all?q=Bacteriorhodopsins","dc:title":"Bacteriorhodopsins"},{"@id":"https://cir.nii.ac.jp/all?q=Lasers","dc:title":"Lasers"},{"@id":"https://cir.nii.ac.jp/all?q=Retinaldehyde","dc:title":"Retinaldehyde"},{"@id":"https://cir.nii.ac.jp/all?q=Stereoisomerism","dc:title":"Stereoisomerism"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1390282680210033152","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"遷移状態を含む光反応・熱反応過程の直接観測に基づく反応機構解明"},{"@language":"en","@value":"Analysis of Reaction Mechanisms based on Direct Observation of the Molecular Structual Changes during both Poto- and Thermal Reactions including the Transition States"},{"@language":"ja-Kana","@value":"センイ ジョウタイ オ フクム ヒカリ ハンノウ ・ ネツ ハンノウ カテイ ノ チョクセツ カンソク ニ モトズク ハンノウ キコウ カイメイ"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282680740585856","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Microbial rhodopsins: wide distribution, rich diversity and great potential"}]},{"@id":"https://cir.nii.ac.jp/crid/1390569457941388032","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Advanced time-resolved absorption spectroscopy with an ultrashort visible/near IR laser and a multi-channel lock-in detector"}]},{"@id":"https://cir.nii.ac.jp/crid/1521980704682237440","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Award Accounts : The Chemical Society of Japan Award for Young Chemists for 2014 : Development of Novel Reactions Induced by Coherent Molecular Vibrational Excitation and Direct Observation of Molecular Structural Change during \"Thermal\" Reactions"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1562/2006-08-19-ir-1006"},{"@type":"OPENAIRE","@value":"doi_dedup___::de7bbafaca1126bc551c6f1a23d5b7bc"},{"@type":"CROSSREF","@value":"10.2142/biophysico.12.0_121_references_DOI_Ox5lGqCAM8hA7k6FpFa6znIoWGr"},{"@type":"CROSSREF","@value":"10.5939/sjws.16003_references_DOI_Ox5lGqCAM8hA7k6FpFa6znIoWGr"},{"@type":"CROSSREF","@value":"10.2183/pjab.97.014_references_DOI_Ox5lGqCAM8hA7k6FpFa6znIoWGr"},{"@type":"CROSSREF","@value":"10.1246/bcsj.20150242_references_DOI_Ox5lGqCAM8hA7k6FpFa6znIoWGr"}]}