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- Michaela Scherr
- From the Department of Hematology and Oncology, Hannover Medical School, Germany; and the Department of Molecular Biology, University of Tübingen, Germany.
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- Karin Battmer
- From the Department of Hematology and Oncology, Hannover Medical School, Germany; and the Department of Molecular Biology, University of Tübingen, Germany.
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- Thomas Winkler
- From the Department of Hematology and Oncology, Hannover Medical School, Germany; and the Department of Molecular Biology, University of Tübingen, Germany.
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- Olaf Heidenreich
- From the Department of Hematology and Oncology, Hannover Medical School, Germany; and the Department of Molecular Biology, University of Tübingen, Germany.
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- Arnold Ganser
- From the Department of Hematology and Oncology, Hannover Medical School, Germany; and the Department of Molecular Biology, University of Tübingen, Germany.
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- Matthias Eder
- From the Department of Hematology and Oncology, Hannover Medical School, Germany; and the Department of Molecular Biology, University of Tübingen, Germany.
説明
<jats:p>Small interfering RNAs (siRNAs) were designed to target thebcr-abl oncogene, which causes chronic myeloid leukemia (CML) and bcr-abl–positive acute lymphoblastic leukemia (ALL). Chemically synthesized anti–bcr-abl siRNAs were selected using reporter gene constructs and were found to reduce bcr-abl mRNA up to 87% in bcr-abl–positive cell lines and in primary cells from CML patients. This mRNA reduction was specific for bcr-abl because c-abl and c-bcr mRNA levels remained unaffected. Furthermore, protein expression of BCR-ABL and of laminA/C was reduced by specific siRNAs up to 80% in bcr-abl–positive and normal CD34+ cells, respectively. Finally, anti–bcr-abl siRNA inhibited BCR-ABL–dependent, but not cytokine-dependent, proliferation in a bcr-abl–positive cell line. These data demonstrate that siRNA can specifically and efficiently interfere with the expression of an oncogenic fusion gene in hematopoietic cells.</jats:p>
収録刊行物
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- Blood
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Blood 101 (4), 1566-1569, 2003-02-15
American Society of Hematology
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詳細情報 詳細情報について
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- CRID
- 1362825894807576960
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- NII論文ID
- 30022494614
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- ISSN
- 15280020
- 00064971
- http://id.crossref.org/issn/00064971
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- データソース種別
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- Crossref
- CiNii Articles