{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362825894933043840.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1111/j.1600-0854.2006.00490.x"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1600-0854.2006.00490.x"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1600-0854.2006.00490.x"}}],"dc:title":[{"@value":"Ternary SNARE Complexes Are Enriched in Lipid Rafts during Mast Cell Exocytosis"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p><jats:bold>Lipid rafts are membrane microdomains rich in cholesterol and glycosphingolipids that have been implicated in the regulation of intracellular protein trafficking. During exocytosis, a class of proteins termed SNAREs mediate secretory granule–plasma membrane fusion. To investigate the role of lipid rafts in secretory granule exocytosis, we examined the raft association of SNARE proteins and SNARE complexes in rat basophilic leukemia (RBL) mast cells. The SNARE protein SNAP‐23 co‐localized with a lipid raft marker and was present in detergent‐insoluble lipid raft microdomains in RBL cells. By contrast, only small amounts (<20%) of the plasma membrane SNARE syntaxin 4 or the granule‐associated SNARE vesicle‐associated membrane protein (VAMP)‐2 were present in these microdomains. Despite this, essentially all syntaxin 4 and most of VAMP‐2 in these rafts were present in SNARE complexes containing SNAP‐23, while essentially none of these complexes were present in nonraft membranes. Whereas SNAP‐23 is membrane anchored by palmitoylation, the association of the transmembrane protein syntaxin 4 with lipid rafts was because of its binding to SNAP‐23. After stimulating mast cells exocytosis, the amount of syntaxin 4 and VAMP‐2 present in rafts increased twofold, and these proteins were now present in raft‐associated phospho‐SNAP‐23/syntaxin 4/VAMP‐2 complexes, revealing differential association of SNARE fusion complexes during the process of regulated exocytosis.</jats:bold></jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382825894933043840","@type":"Researcher","foaf:name":[{"@value":"Niti Puri"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825894933043841","@type":"Researcher","foaf:name":[{"@value":"Paul A. Roche"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"13989219"},{"@type":"EISSN","@value":"16000854"}],"prism:publicationName":[{"@value":"Traffic"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2006-09-19","prism:volume":"7","prism:number":"11","prism:startingPage":"1482","prism:endingPage":"1494"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1600-0854.2006.00490.x"},{"@id":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1600-0854.2006.00490.x"}],"createdAt":"2006-09-19","modifiedAt":"2023-10-14","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004233482177536","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Rab5 is critical for SNAP23 regulated granule-granule fusion during compound exocytosis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004235034493568","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Budding transition of asymmetric two-component lipid domains"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283690771443456","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Omalizumab inhibits acceleration of FcεRI-mediated responsiveness of immature human mast cells by immunoglobulin E"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285706983353088","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The suppression of IgE-mediated histamine release from mast cells following exocytic exclusion of biodegradable polymeric nanoparticles"}]},{"@id":"https://cir.nii.ac.jp/crid/1360290617731089152","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Rab44 isoforms similarly promote lysosomal exocytosis, but exhibit differential localization in mast cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848657144287872","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Liprin-α is involved in exocytosis and cell spreading in mast cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848659427203200","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Phosphorylation of SNAP-23 at Ser95 causes a structural alteration and negatively regulates Fc receptor–mediated phagosome formation and maturation in macrophages"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679609557120","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Effect of Complexin II on Membrane Fusion between Liposomes Containing Mast Cell SNARE Proteins"}]},{"@id":"https://cir.nii.ac.jp/crid/1390575108414215552","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"SNAP23-Mediated Perturbation of Cholesterol-Enriched Membrane Microdomain Promotes Extracellular Vesicle Production in Src-Activated Cancer Cells"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1111/j.1600-0854.2006.00490.x"},{"@type":"CROSSREF","@value":"10.1038/s41598-017-15047-8_references_DOI_JLHnRaip0wKPjvJd1h2SekvsssQ"},{"@type":"CROSSREF","@value":"10.1103/physreve.94.032406_references_DOI_JLHnRaip0wKPjvJd1h2SekvsssQ"},{"@type":"CROSSREF","@value":"10.1016/j.biomaterials.2011.09.043_references_DOI_JLHnRaip0wKPjvJd1h2SekvsssQ"},{"@type":"CROSSREF","@value":"10.1016/j.imlet.2011.05.010_references_DOI_JLHnRaip0wKPjvJd1h2SekvsssQ"},{"@type":"CROSSREF","@value":"10.1002/2211-5463.13133_references_DOI_JLHnRaip0wKPjvJd1h2SekvsssQ"},{"@type":"CROSSREF","@value":"10.1248/bpb.b22-00560_references_DOI_JLHnRaip0wKPjvJd1h2SekvsssQ"},{"@type":"CROSSREF","@value":"10.1091/mbc.e17-08-0523_references_DOI_JLHnRaip0wKPjvJd1h2SekvsssQ"},{"@type":"CROSSREF","@value":"10.1016/j.anai.2012.01.009_references_DOI_JLHnRaip0wKPjvJd1h2SekvsssQ"},{"@type":"CROSSREF","@value":"10.1248/bpb.b15-00751_references_DOI_JLHnRaip0wKPjvJd1h2SekvsssQ"}]}