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- Ian M. Slaymaker
- Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
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- Linyi Gao
- Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
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- Bernd Zetsche
- Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
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- David A. Scott
- Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
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- Winston X. Yan
- Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
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- Feng Zhang
- Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
説明
<jats:title>Making the correct cut</jats:title> <jats:p> The CRISPR/Cas system is a prokaryotic immune system that targets and cuts out foreign DNA in bacteria. It has been adopted for gene editing because it can be designed to recognize and cut specific locations in the genome. A challenge in developing clinical applications is the potential for off-target effects that could result in DNA cleavage at the wrong locations. Slaymaker <jats:italic>et al.</jats:italic> used structure-guided engineering to improve the specificity of <jats:italic>Streptococcus pyogenes</jats:italic> Cas9 (SpCas9). They identified enhanced-specificity variants (eSpCas9) that display reduced off-target cleavage while maintaining robust on-target activity </jats:p> <jats:p> <jats:italic>Science</jats:italic> , this issue p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6268" page="84" related-article-type="in-this-issue" vol="351" xlink:href="10.1126/science.aad5227">84</jats:related-article> </jats:p>
収録刊行物
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- Science
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Science 351 (6268), 84-88, 2016-01
American Association for the Advancement of Science (AAAS)