{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362825895155504128.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1242/jcs.00836"}},{"identifier":{"@type":"URI","@value":"http://journals.biologists.com/jcs/article-pdf/117/1/41/1363317/41.pdf"}}],"dc:title":[{"@value":"Cell behaviour on micropatterned substrata: limits of extracellular matrix geometry for spreading and adhesion"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>Cell adhesion, spreading and migration require the dynamic formation and dispersal of contacts with the extracellular matrix (ECM). In vivo, the number, availability and distribution of ECM binding sites dictate the shape of a cell and determine its mobility. To analyse the geometrical limits of ECM binding sites required for cell attachment and spreading, we used microcontact printing to produce regular patterns of ECM protein dots of defined size separated by nonadhesive regions. Cells cultured on these substrata adhere to and spread on ECM regions as small as 0.1 μm2, when spacing between dots is less than 5 μm. Spacing of 5-25 μm induces a cell to adapt its shape to the ECM pattern. The ability to spread and migrate on dots ≥1 μm2 ceases when the dot separation is ≥30 μm. The extent of cell spreading is directly correlated to the total substratum coverage with ECM-proteins, but irrespective of the geometrical pattern. An optimal spreading extent is reached at a surface coating above 15%. Knowledge of these geometrical limits is essential for an understanding of cell adhesion and migration, and for the design of artificial surfaces that optimally interact with cells in a living tissue.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382825895155504130","@type":"Researcher","foaf:name":[{"@value":"Dirk Lehnert"}],"jpcoar:affiliationName":[{"@value":"Department of Biology, University of Konstanz, Universitaetstrasse 10, 78457 Konstanz, Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825895155504133","@type":"Researcher","foaf:name":[{"@value":"Bernhard Wehrle-Haller"}],"jpcoar:affiliationName":[{"@value":"Department of Pathology, Centre Medical Universitaire, Geneva, Switzerland"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825895155504128","@type":"Researcher","foaf:name":[{"@value":"Christian David"}],"jpcoar:affiliationName":[{"@value":"Laboratory for Micro- and Nanotechnology, Paul Scherrer Institut, Villigen-PSI, Switzerland"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825895155504134","@type":"Researcher","foaf:name":[{"@value":"Ulrich Weiland"}],"jpcoar:affiliationName":[{"@value":"Department of Biology, University of Konstanz, Universitaetstrasse 10, 78457 Konstanz, Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825895155504129","@type":"Researcher","foaf:name":[{"@value":"Christoph Ballestrem"}],"jpcoar:affiliationName":[{"@value":"Department of Pathology, Centre Medical Universitaire, Geneva, Switzerland"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825895155504132","@type":"Researcher","foaf:name":[{"@value":"Beat A. Imhof"}],"jpcoar:affiliationName":[{"@value":"Department of Pathology, Centre Medical Universitaire, Geneva, Switzerland"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825895155504131","@type":"Researcher","foaf:name":[{"@value":"Martin Bastmeyer"}],"jpcoar:affiliationName":[{"@value":"Department of Biology, University of Konstanz, Universitaetstrasse 10, 78457 Konstanz, Germany"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"14779137"},{"@type":"PISSN","@value":"00219533"}],"prism:publicationName":[{"@value":"Journal of Cell Science"}],"dc:publisher":[{"@value":"The Company of Biologists"}],"prism:publicationDate":"2004-01-01","prism:volume":"117","prism:number":"1","prism:startingPage":"41","prism:endingPage":"52"},"reviewed":"false","url":[{"@id":"http://journals.biologists.com/jcs/article-pdf/117/1/41/1363317/41.pdf"}],"createdAt":"2003-12-02","modifiedAt":"2021-06-13","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050001202554369664","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Discriminating the Independent Influence of Cell Adhesion and Spreading Area on Stem Cell Fate Determination Using Micropatterned Surfaces"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004232367013888","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Differentiating stem cells on patterned substrates for neural network 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