{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362825895367864192.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1073/pnas.0406909101"}},{"identifier":{"@type":"URI","@value":"https://pnas.org/doi/pdf/10.1073/pnas.0406909101"}}],"dc:title":[{"@value":"Inorganic polyphosphate in the origin and survival of species"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>\n            Inorganic polyphosphate (poly P), in chains of tens to hundreds of phosphate residues, linked by high-energy bonds, is environmentally ubiquitous and abundant. In prebiotic evolution it could have provided a flexible, polyanionic scaffold to assemble macromolecules. It has been conserved in every cell in nature. In prokaryotes, a major poly P synthetic enzyme is poly P kinase 1 (PPK1), which is found in 100 bacterial genomes, including numerous pathogens. Null mutants of PPK1, with low poly P levels, are defective in survival: namely, they show defective responses to physical/chemical stresses and predation. Pathogens with a PPK1 deletion are defective in biofilm formation, quorum sensing, general stress and stringent responses, motility, and other virulence properties. With the exception of\n            <jats:italic>Dictyostelium</jats:italic>\n            , PPK1 is absent in eukaryotes and provides a novel target for chemotherapy that would affect both virulence and susceptibility to antibacterial compounds. Remarkably, another PPK in\n            <jats:italic>Dictyostelium discoideum</jats:italic>\n            (PPK2) is an actin-related protein (Arp) complex that is polymerized into an actin-like filament, concurrent with its reversible synthesis of a poly P chain from ATP.\n          </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380576121151121921","@type":"Researcher","foaf:name":[{"@value":"Michael R. W. Brown"}],"jpcoar:affiliationName":[{"@value":"Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305-5307"}]},{"@id":"https://cir.nii.ac.jp/crid/1380576121151121920","@type":"Researcher","foaf:name":[{"@value":"Arthur Kornberg"}],"jpcoar:affiliationName":[{"@value":"Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305-5307"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00278424"},{"@type":"EISSN","@value":"10916490"}],"prism:publicationName":[{"@value":"Proceedings of the National Academy of Sciences"}],"dc:publisher":[{"@value":"Proceedings of the National Academy of Sciences"}],"prism:publicationDate":"2004-11","prism:volume":"101","prism:number":"46","prism:startingPage":"16085","prism:endingPage":"16087"},"reviewed":"false","url":[{"@id":"https://pnas.org/doi/pdf/10.1073/pnas.0406909101"}],"createdAt":"2004-11-03","modifiedAt":"2022-04-12","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050021249136360448","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Monovalent Ion Effect on Liquid-Liquid Phase Separation of Aqueous Polyphosphate-Salt Mixtures"}]},{"@id":"https://cir.nii.ac.jp/crid/1360284919142932736","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Polyphosphate metabolism by purple non-sulfur bacteria and its possible application on photo-microbial fuel cell"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1073/pnas.0406909101"},{"@type":"CROSSREF","@value":"10.1016/j.jbiosc.2017.01.012_references_DOI_CcniE1eY0RtVNsUJNQbJCdgcNWM"},{"@type":"CROSSREF","@value":"10.1021/acs.jpcb.4c03457_references_DOI_CcniE1eY0RtVNsUJNQbJCdgcNWM"}]}