{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362825895436516992.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1159/000228409"}},{"identifier":{"@type":"URI","@value":"https://www.karger.com/Article/Pdf/228409"}}],"dc:title":[{"@value":"Effect of dsRNA on Mesangial Cell Synthesis of Plasminogen Activator Inhibitor Type 1 and Tissue Plasminogen Activator"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p><i>Background/Aims:</i> Viral infections are a major problem worldwide and many of them are complicated by virally induced glomerulonephritides. Progression of kidney disease to renal failure is mainly attributed to the development of renal fibrosis characterized by the accumulation of extracellular matrix components in the mesangial cell compartment and the glomerular basement membrane. Plasminogen activator inhibitor type 1 (PAI-1) and tissue plasminogen activator (t-PA) are major regulators of plasmin generation and play an important role in generation and degradation of glomerular extracellular matrix components. Viral receptors expressed by mesangial cells (MC) are known to be key mediators in immune-mediated glomerulonephritis. We investigated the effect of stimulation of the viral receptors toll-like receptor 3 (TLR3) and retinoic acid-inducible gene I (RIG-I) on the expression of PAI-1 and t-PA. <i>Methods:</i> Expression of PAI-1 and t-PA in immortalized human MC stimulated with polyriboinosinic:polyribocytidylic acid [poly(I:C)] RNA and cytokines were analyzed by real-time RT-PCR and ELISA. <i>Results:</i> Incubation of MC with poly(I:C) RNA to activate the viral receptors TLR3 and RIG-I upregulates the expression of PAI-1 and t-PA. Knockdown of viral receptors with specific siRNA abolishes the induction of PAI-1 and t-PA. <i>Conclusion:</i> For the first time a link between the activation of viral receptors on MC and potentially causative agents in the development of glomerulosclerosis and tubulointerstitial fibrosis is shown. The progression of inflammatory processes to glomerulosclerosis can be postulated to be directly enhanced by viral infection.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380013199267363712","@type":"Researcher","foaf:name":[{"@value":"Markus Wörnle"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825895436516995","@type":"Researcher","foaf:name":[{"@value":"Maximilian Roeder"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825895436516996","@type":"Researcher","foaf:name":[{"@value":"Matthias Sauter"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825895436516993","@type":"Researcher","foaf:name":[{"@value":"Monika Merkle"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825895436516994","@type":"Researcher","foaf:name":[{"@value":"Andrea Ribeiro"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"16602129"}],"prism:publicationName":[{"@value":"Nephron Experimental Nephrology"}],"dc:publisher":[{"@value":"S. Karger AG"}],"prism:publicationDate":"2009-07-10","prism:volume":"113","prism:number":"2","prism:startingPage":"e57","prism:endingPage":"e65"},"reviewed":"false","dc:rights":["https://www.karger.com/Services/SiteLicenses","https://www.karger.com/Services/SiteLicenses"],"url":[{"@id":"https://www.karger.com/Article/Pdf/228409"}],"createdAt":"2009-07-10","modifiedAt":"2025-04-23","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004236679274624","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Retinoic acid-inducible gene-I (RIG-I) is induced by IFN-γ in human mesangial cells in culture: possible involvement of RIG-I in the inflammation in lupus nephritis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565168623308928","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Inflammatory Chemokine Expression via Toll-Like Receptor 3 Signaling in Normal Human Mesangial Cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679315122432","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Expression of retinoic acid-inducible gene-I (RIG-I) in cultured human mesangial cells: A possible involvement of RIG-I in the inflammatory reactions"},{"@language":"ja","@value":"ヒト培養メサンギウム細胞におけるｒｅｔｉｎｏｉｃ　ａｃｉｄ‐ｉｎｄｕｃｉｂｌｅ　ｇｅｎｅ‐Ｉの発現と炎症過程への関与"}]},{"@id":"https://cir.nii.ac.jp/crid/2051996266985195904","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Chloroquine attenuates TLR3-mediated plasminogen activator inhibitor-1 expression in cultured human glomerular endothelial cells"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1159/000228409"},{"@type":"CROSSREF","@value":"10.1007/s10157-018-1659-y_references_DOI_Pg2RbRZ1isJimbKxbbdvYjxwBCV"},{"@type":"CROSSREF","@value":"10.3165/jjpn.23.141_references_DOI_Pg2RbRZ1isJimbKxbbdvYjxwBCV"},{"@type":"CROSSREF","@value":"10.1155/2013/984708_references_DOI_Pg2RbRZ1isJimbKxbbdvYjxwBCV"},{"@type":"CROSSREF","@value":"10.1177/0961203309360540_references_DOI_Pg2RbRZ1isJimbKxbbdvYjxwBCV"}]}