{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362825895482860032.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1083/jcb.105.6.2735"}},{"identifier":{"@type":"URI","@value":"https://rupress.org/jcb/article-pdf/105/6/2735/1466264/2735.pdf"}},{"identifier":{"@type":"NAID","@value":"30017411042"}}],"dc:title":[{"@value":"Constitutive apical secretion of an 80-kD sulfated glycoprotein complex in the polarized epithelial Madin-Darby canine kidney cell line."}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>The biosynthesis, processing, and apical secretion of a group of polypeptides (Kondor-Koch, C., R. Bravo, S. D. Fuller, D. Cutler, and H. Garoff. 1985. Cell. 43:297-306) are studied in MDCK cells using a specific polyclonal antiserum. These polypeptides are synthesized as a precursor protein which has an apparent Mr of 65,000 in its high mannose form. This precursor is converted into a protein with an apparent Mr of 80,000 containing complex carbohydrates and sulfate. After intracellular cleavage of the 80-kD protein, the 35-45-kD subunits are secreted as an 80-kD glycoprotein complex (gp 80) linked together by disulfide bonds. Secretion of the protein complex occurs by a constitutive pathway at the apical surface of the epithelial monolayer. Since the immediate post-translational precursor, the 65-kD protein, is hydrophilic in nature as shown by its partitioning behavior in a phase-separated Triton X-114 solution, gp 80 is segregated into the apical exocytotic pathway as a soluble molecule. The proteolytic maturation of gp 80 is blocked in the presence of chloroquine and its secretion is retarded. The 80-kD precursor is released at the apical cell surface, demonstrating that proteolytic processing is not necessary for the apical secretion of this protein. If N-glycosylation is inhibited by tunicamycin treatment the protein is secreted in equal amounts at both cell surfaces, indicating a role of the carbohydrate moieties in the vectorial transport of this protein.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1581698599091883392","@type":"Researcher","foaf:name":[{"@value":"J Urban"}],"jpcoar:affiliationName":[{"@value":"Abt. Molekulare Genetik, Universität Frankfurt, Federal Republic of Germany."}]},{"@id":"https://cir.nii.ac.jp/crid/1382825895482860032","@type":"Researcher","foaf:name":[{"@value":"K Parczyk"}],"jpcoar:affiliationName":[{"@value":"Abt. Molekulare Genetik, Universität Frankfurt, Federal Republic of Germany."}]},{"@id":"https://cir.nii.ac.jp/crid/1382825895482860036","@type":"Researcher","foaf:name":[{"@value":"A Leutz"}],"jpcoar:affiliationName":[{"@value":"Abt. Molekulare Genetik, Universität Frankfurt, Federal Republic of Germany."}]},{"@id":"https://cir.nii.ac.jp/crid/1382825895482860033","@type":"Researcher","foaf:name":[{"@value":"M Kayne"}],"jpcoar:affiliationName":[{"@value":"Abt. Molekulare Genetik, Universität Frankfurt, Federal Republic of Germany."}]},{"@id":"https://cir.nii.ac.jp/crid/1382825895482860035","@type":"Researcher","foaf:name":[{"@value":"C Kondor-Koch"}],"jpcoar:affiliationName":[{"@value":"Abt. Molekulare Genetik, Universität Frankfurt, Federal Republic of Germany."}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00219525"},{"@type":"EISSN","@value":"15408140"}],"prism:publicationName":[{"@value":"The Journal of cell biology"}],"dc:publisher":[{"@value":"Rockefeller University Press"}],"prism:publicationDate":"1987-12-01","prism:volume":"105","prism:number":"6","prism:startingPage":"2735","prism:endingPage":"2743"},"reviewed":"false","url":[{"@id":"https://rupress.org/jcb/article-pdf/105/6/2735/1466264/2735.pdf"}],"createdAt":"2004-05-15","modifiedAt":"2023-07-22","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360009142672228096","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The c‐terminal region of BLT2 restricts its localization to the lateral membrane in a LIN7C‐dependent manner"}]},{"@id":"https://cir.nii.ac.jp/crid/1390856893076176256","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"ChREBP deficiency prevents high sucrose diet-induced obesity through reducing sucrase expression"}]},{"@id":"https://cir.nii.ac.jp/crid/1520854805757496064","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@value":"スクラーゼーイソマルターゼ:細胞生物学的過程を研究するためのユニークなモデル〔原文は英文〕"},{"@language":"ja-Kana","@value":"スクラーゼーイソマルターゼ サイボウ セイブツガクテキ カテイ オ ケンキュウ"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1083/jcb.105.6.2735"},{"@type":"CIA","@value":"30017411042"},{"@type":"CROSSREF","@value":"10.1096/fj.202002640r_references_DOI_ZE0bTME2VzUBKfma0KGvgq2gNJP"},{"@type":"CROSSREF","@value":"10.3164/jcbn.22-15_references_DOI_ZE0bTME2VzUBKfma0KGvgq2gNJP"}]}