{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362825895694030848.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1152/ajpcell.1997.273.4.c1109"}},{"identifier":{"@type":"URI","@value":"https://journals.physiology.org/doi/pdf/10.1152/ajpcell.1997.273.4.C1109"}}],"dc:title":[{"@value":"Three-dimensional cell cultures: from molecular mechanisms to clinical applications"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>This article reviews actual advances in the development and application of three-dimensional (3-D) cell culture systems. Recent therapeutically oriented studies include characterization of multicellular-mediated drug resistance, novel ways of quantifying hypoxia, and new approaches to more efficient immunotherapy. Recent progress toward understanding the development of necrosis in tumor spheroids has been made using novel spheroid models. 3-D cultures have been used for studies on molecular mechanisms involved in invasion and metastasis, with a major focus on the role of E-cadherin. Similarly, tumor angiogenesis and the significance of vascular endothelial growth factor have been investigated in a variety of 3-D culture systems. There are many ongoing developments in tissue modeling or remodeling that promise significant progress toward the development of bioartificial liver support and artificial blood. Perhaps one of the most interesting areas of basic research with 3-D cultures is the characterization of embryoid bodies obtained from stable embryonic stem cells. These models have greatly increased the understanding of embryonic development, in particular through the notable exceptional advances in cardiogenesis.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382825895694030848","@type":"Researcher","foaf:name":[{"@value":"Wolfgang Mueller-Klieser"}],"jpcoar:affiliationName":[{"@value":"Institute of Physiology and Pathophysiology, Johannes Gutenberg-University Mainz, D-55099 Mainz, Germany"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"03636143"},{"@type":"EISSN","@value":"15221563"}],"prism:publicationName":[{"@value":"American Journal of Physiology-Cell Physiology"}],"dc:publisher":[{"@value":"American Physiological Society"}],"prism:publicationDate":"1997-10-01","prism:volume":"273","prism:number":"4","prism:startingPage":"C1109","prism:endingPage":"C1123"},"reviewed":"false","url":[{"@id":"https://journals.physiology.org/doi/pdf/10.1152/ajpcell.1997.273.4.C1109"}],"createdAt":"2017-12-24","modifiedAt":"2025-06-29","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360002217131214720","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Retaining cell–cell contact enables preparation and culture of spheroids composed of pure primary cancer cells from colorectal cancer"}]},{"@id":"https://cir.nii.ac.jp/crid/1360002218683499904","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"TRIB1 Supports Prostate Tumorigenesis and Tumor-Propagating Cell Survival by Regulation of Endoplasmic Reticulum Chaperone Expression"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004232000762624","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The usefulness of three-dimensional cell culture in induction of cancer stem cells from esophageal squamous cell carcinoma cell lines"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004234484455424","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Around 90° Contact Angle of Dish Surface Is a Key Factor in Achieving Spontaneous Spheroid Formation"}]},{"@id":"https://cir.nii.ac.jp/crid/1360017282450075776","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Intracerebral Transplantation of Mesenchymal Stromal Cell Compounded with Recombinant Peptide Scaffold against Chronic Intracerebral Hemorrhage Model"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285711337293184","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Spontaneously Formed Spheroids from Mouse Compact Bone-Derived Cells Retain Highly Potent Stem Cells with Enhanced Differentiation Capability"}]},{"@id":"https://cir.nii.ac.jp/crid/1360294643837138944","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Quality Verification with a Cluster−Controlled Manufacturing System to Generate Monocyte−Derived Dendritic Cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360576118842423680","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"One-step visualization of natural cell activities in non-labeled living spheroids"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001206126763904","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"3次元培養における前立腺がん細胞の生物学的特性"},{"@language":"en","@value":"The Biological Behavior of Prostate Cancer Cells in 3D Culture Systems"},{"@language":"ja-Kana","@value":"3ジゲン バイヨウ ニ オケル ゼンリツセン ガン サイボウ ノ セイブツガクテキ トクセイ"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1152/ajpcell.1997.273.4.c1109"},{"@type":"CROSSREF","@value":"10.1155/2022/8521922_references_DOI_EUHGNJnqKQZ8gw6tJ2TjoDMqeot"},{"@type":"CROSSREF","@value":"10.1155/2019/8469012_references_DOI_EUHGNJnqKQZ8gw6tJ2TjoDMqeot"},{"@type":"CROSSREF","@value":"10.3390/vaccines9050533_references_DOI_EUHGNJnqKQZ8gw6tJ2TjoDMqeot"},{"@type":"CROSSREF","@value":"10.1073/pnas.1015938108_references_DOI_EUHGNJnqKQZ8gw6tJ2TjoDMqeot"},{"@type":"CROSSREF","@value":"10.1016/j.bbrc.2013.04.008_references_DOI_EUHGNJnqKQZ8gw6tJ2TjoDMqeot"},{"@type":"CROSSREF","@value":"10.1038/s41598-022-05347-z_references_DOI_EUHGNJnqKQZ8gw6tJ2TjoDMqeot"},{"@type":"CROSSREF","@value":"10.1089/ten.tec.2018.0188_references_DOI_EUHGNJnqKQZ8gw6tJ2TjoDMqeot"},{"@type":"CROSSREF","@value":"10.1158/0008-5472.can-13-3718_references_DOI_EUHGNJnqKQZ8gw6tJ2TjoDMqeot"},{"@type":"CROSSREF","@value":"10.1248/yakushi.128.37_references_DOI_EUHGNJnqKQZ8gw6tJ2TjoDMqeot"}]}