{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362825895755774464.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1128/aem.00931-10"}},{"identifier":{"@type":"URI","@value":"https://journals.asm.org/doi/pdf/10.1128/AEM.00931-10"}}],"dc:title":[{"@value":"Exploring Mechanisms of Resistance to Respiratory Inhibitors in Field Strains of\n            <i>Botrytis cinerea</i>\n            , the Causal Agent of Gray Mold"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            Respiratory inhibitors are among the fungicides most widely used for disease control on crops. Most are strobilurins and carboxamides, inhibiting the cytochrome\n            <jats:italic>b</jats:italic>\n            of mitochondrial complex III and the succinate dehydrogenase of mitochondrial complex II, respectively. A few years after the approval of these inhibitors for use on grapevines, field isolates of\n            <jats:italic>Botrytis cinerea</jats:italic>\n            , the causal agent of gray mold, resistant to one or both of these classes of fungicide were recovered in France and Germany. However, little was known about the mechanisms underlying this resistance in field populations of this fungus. Such knowledge could facilitate resistance risk assessment. The aim of this study was to investigate the mechanisms of resistance occurring in\n            <jats:italic>B. cinerea</jats:italic>\n            populations. Highly specific resistance to strobilurins was correlated with a single mutation of the\n            <jats:italic>cytb</jats:italic>\n            target gene. Changes in its intronic structure may also have occurred due to an evolutionary process controlling selection for resistance. Specific resistance to carboxamides was identified for six phenotypes, with various patterns of resistance levels and cross-resistance. Several mutations specific to\n            <jats:italic>B. cinerea</jats:italic>\n            were identified within the\n            <jats:italic>sdhB</jats:italic>\n            and\n            <jats:italic>sdhD</jats:italic>\n            genes encoding the iron-sulfur protein and an anchor protein of the succinate dehydrogenase complex. Another as-yet-uncharacterized mechanism of resistance was also recorded. In addition to target site resistance mechanisms, multidrug resistance, linked to the overexpression of membrane transporters, was identified in strains with low to moderate resistance to several respiratory inhibitors. This diversity of resistance mechanisms makes resistance management difficult and must be taken into account when developing strategies for\n            <jats:italic>Botrytis</jats:italic>\n            control.\n          </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382825895755774467","@type":"Researcher","foaf:name":[{"@value":"Pierre Leroux"}],"jpcoar:affiliationName":[{"@value":"INRA-UR 1290 BIOGER-CPP, Avenue Lucien Bretignières BP 01, F78850 Thiverval-Grignon, France"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825895755774465","@type":"Researcher","foaf:name":[{"@value":"Michel Gredt"}],"jpcoar:affiliationName":[{"@value":"INRA-UR 1290 BIOGER-CPP, Avenue Lucien Bretignières BP 01, F78850 Thiverval-Grignon, France"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825895755774466","@type":"Researcher","foaf:name":[{"@value":"Michaela Leroch"}],"jpcoar:affiliationName":[{"@value":"Technische Universität Kaiserslautern, Fachbereich Biologie, Postfach 3049, D67653 Kaiserslautern, Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825895755774464","@type":"Researcher","foaf:name":[{"@value":"Anne-Sophie Walker"}],"jpcoar:affiliationName":[{"@value":"INRA-UR 1290 BIOGER-CPP, Avenue Lucien Bretignières BP 01, F78850 Thiverval-Grignon, France"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00992240"},{"@type":"EISSN","@value":"10985336"}],"prism:publicationName":[{"@value":"Applied and Environmental Microbiology"}],"dc:publisher":[{"@value":"American Society for Microbiology"}],"prism:publicationDate":"2010-10","prism:volume":"76","prism:number":"19","prism:startingPage":"6615","prism:endingPage":"6630"},"reviewed":"false","dc:rights":["https://journals.asm.org/non-commercial-tdm-license"],"url":[{"@id":"https://journals.asm.org/doi/pdf/10.1128/AEM.00931-10"}],"createdAt":"2010-08-07","modifiedAt":"2022-02-23","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1390282680185988096","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Strategy for discovery of a novel miticide Cyenopyrafen which is one of electron transport chain inhibitors"},{"@value":"電子伝達系を作用点とする新規殺ダニ剤Cyenopyrafen：抵抗性回避を目指した探索戦略"},{"@language":"ja-Kana","@value":"デンシ デンタツケイ オ サヨウテン ト スル シンキサツダニザイ Cyenopyrafen:テイコウセイ カイヒ オ メザシタ タンサク センリャク"},{"@language":"ja","@value":"電子伝達系を作用点とする新規殺ダニ剤Cyenopyrafen : 抵抗性回避を目指した探索戦略(作用点から見た農薬科学の新展開)"}]},{"@id":"https://cir.nii.ac.jp/crid/1390588822821073920","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"三重県における果菜類灰色かび病菌の各種SDHI剤に対する交差耐性およびイソフェタミド耐性系統の発生動向の把握"},{"@language":"en","@value":"Trends in cross-resistance to SDHI fungicides and occurrence of isofetamid resistance in <i>Botrytis cinerea</i> isolates in Mie Prefecture, Japan"},{"@language":"ja-Kana","@value":"ミエケン ニ オケル カサイルイ ハイイロカビ ビョウキン ノ カクシュ SDHIザイ ニ タイスル コウサ タイセイ オヨビ イソフェタミド タイセイ ケイトウ ノ ハッセイ ドウコウ ノ ハアク"}]},{"@id":"https://cir.nii.ac.jp/crid/1390851087356709248","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Aminopyrifen, a novel 2-aminonicotinate fungicide with a unique effect and broad-spectrum activity against plant pathogenic fungi"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1128/aem.00931-10"},{"@type":"CROSSREF","@value":"10.3186/jjphytopath.92.1_references_DOI_6JK52mkMJtWYuqfjRGBNYlYweBl"},{"@type":"CROSSREF","@value":"10.1584/jpestics.d20-094_references_DOI_6JK52mkMJtWYuqfjRGBNYlYweBl"},{"@type":"CROSSREF","@value":"10.1584/jpestics.w11-34_references_DOI_6JK52mkMJtWYuqfjRGBNYlYweBl"}]}