{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362825896109606016.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1111/j.1600-6143.2008.02287.x"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1600-6143.2008.02287.x"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S1600613522055800?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S1600613522055800?httpAccept=text/plain"}},{"identifier":{"@type":"URI","@value":"http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1600-6143.2008.02287.x/fullpdf"}},{"identifier":{"@type":"PMID","@value":"18557739"}}],"dc:title":[{"@value":"Increase of Integrin-Linked Kinase Activity in Cultured Podocytes upon Stimulation with Plasma from Patients with Recurrent FSGS"}],"description":[{"notation":[{"@value":"Recurrent focal segmental glomerulosclerosis (FSGS) is a major challenge in the field of transplantation. Integrin-linked kinase (ILK) has emerged as a key mediator of podocyte-glomerular basement membrane (GBM) interactions. To clarify the involvement of plasma factors in FSGS recurrence, we examined the effects of plasma from FSGS patients with or without posttransplant recurrence on cultured podocytes, focusing particularly on ILK activity. Podocytes from a conditionally immortalized mouse podocyte cell line were treated with plasma from 11 FSGS patients, and ILK activity was determined using an immune complex kinase assay. Treatment with plasma from three patients with recurrence induced an increase in ILK activity. In contrast, no increase in ILK activity was observed in cultured podocytes treated with plasma from the remaining three patients with recurrence and five patients without recurrence. Cultured podocytes treated with plasma that induced ILK activity showed alterations of focal contact and detachment from the laminin matrix. In conclusion, this preliminary study provides experimental evidence suggesting the possible presence of circulating toxic factors in the plasma of some patients with recurrent FSGS, which induce an increase in podocyte ILK activity that may lead to the detachment of podocytes from the GBM."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380285706277595793","@type":"Researcher","foaf:name":[{"@value":"M. Hattori"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825896109606017","@type":"Researcher","foaf:name":[{"@value":"Y. Akioka"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825896109606021","@type":"Researcher","foaf:name":[{"@value":"H. Chikamoto"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825896109606023","@type":"Researcher","foaf:name":[{"@value":"N. Kobayashi"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825896109606018","@type":"Researcher","foaf:name":[{"@value":"K. Tsuchiya"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825896109606019","@type":"Researcher","foaf:name":[{"@value":"M. Shimizu"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825896109606020","@type":"Researcher","foaf:name":[{"@value":"S. Kagami"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825896109606022","@type":"Researcher","foaf:name":[{"@value":"H. Tsukaguchi"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"16006135"}],"prism:publicationName":[{"@value":"American Journal of Transplantation"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"2008-07","prism:volume":"8","prism:number":"7","prism:startingPage":"1550","prism:endingPage":"1556"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/","https://www.elsevier.com/legal/tdmrep-license","http://creativecommons.org/licenses/by-nc-nd/4.0/"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1600-6143.2008.02287.x"},{"@id":"https://api.elsevier.com/content/article/PII:S1600613522055800?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S1600613522055800?httpAccept=text/plain"},{"@id":"http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1600-6143.2008.02287.x/fullpdf"}],"createdAt":"2008-06-13","modifiedAt":"2025-10-28","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Male","dc:title":"Male"},{"@id":"https://cir.nii.ac.jp/all?q=Adolescent","dc:title":"Adolescent"},{"@id":"https://cir.nii.ac.jp/all?q=Glomerulosclerosis,%20Focal%20Segmental","dc:title":"Glomerulosclerosis, Focal Segmental"},{"@id":"https://cir.nii.ac.jp/all?q=Podocytes","dc:title":"Podocytes"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Culture%20Techniques","dc:title":"Cell Culture Techniques"},{"@id":"https://cir.nii.ac.jp/all?q=Infant","dc:title":"Infant"},{"@id":"https://cir.nii.ac.jp/all?q=Protein%20Serine-Threonine%20Kinases","dc:title":"Protein Serine-Threonine Kinases"},{"@id":"https://cir.nii.ac.jp/all?q=Mice","dc:title":"Mice"},{"@id":"https://cir.nii.ac.jp/all?q=Plasma","dc:title":"Plasma"},{"@id":"https://cir.nii.ac.jp/all?q=Recurrence","dc:title":"Recurrence"},{"@id":"https://cir.nii.ac.jp/all?q=Child,%20Preschool","dc:title":"Child, Preschool"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Female","dc:title":"Female"},{"@id":"https://cir.nii.ac.jp/all?q=Child","dc:title":"Child"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360306905171620608","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Clinical Course of Nonresponders With Recurrent Focal Segmental Glomerulosclerosis After Pediatric Kidney Transplantation: A Retrospective Multicenter Study"}]},{"@id":"https://cir.nii.ac.jp/crid/1360572092597912960","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Amount and selectivity of proteinuria may predict the treatment response in post-transplant recurrence of focal segmental glomerulosclerosis: a single-center retrospective study"}]},{"@id":"https://cir.nii.ac.jp/crid/1360576118803722368","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Response to steroid and immunosuppressive therapies may predict post‐transplant recurrence of steroid‐resistant nephrotic syndrome"}]},{"@id":"https://cir.nii.ac.jp/crid/1360857593692833408","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Degree of foot process effacement in patients with genetic focal segmental glomerulosclerosis: a single-center analysis and review of the literature"}]},{"@id":"https://cir.nii.ac.jp/crid/1360862720845973760","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A multi-institutional study found a possible role of anti-nephrin antibodies in post-transplant focal segmental glomerulosclerosis recurrence"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204338136064","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"生体腎移植後のＦＳＧＳ再発で著明なｃｅｌｌｕｌａｒ　ｌｅｓｉｏｎを認め，早期に移植腎機能が廃絶した１女児例：液性因子と臨床病理像に関する検討"},{"@language":"en","@value":"A case of recurrent focal segmental glomerulosclerosis with cellular lesions soon after living-related renal transplantation: A possible association of the plasma circulating factors with clinicopathologic features"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204678188672","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"小児末期慢性腎不全診療の歩みと現況"},{"@value":"小児末期慢性腎不全診療の歩みと現況--第53回日本透析医学会教育講演より"},{"@language":"ja-Kana","@value":"ショウニ マッキ マンセイ ジンフゼン シンリョウ ノ アユミ ト ゲンキョウ ダイ53カイ ニホン トウセキ イガッカイ キョウイク コウエン ヨリ"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282681420496512","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"3. 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