{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1362825896368813440.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1046/j.1365-2958.2003.03549.x"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-2958.2003.03549.x"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-2958.2003.03549.x"}}],"dc:title":[{"@value":"A novel sheathed surface organelle of the <i>Helicobacter pylori cag</i> type IV secretion system"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Summary</jats:title><jats:p>Type I strains of <jats:italic>Helicobacter pylori</jats:italic> (<jats:italic>Hp</jats:italic>) use a type IV secretion system (T4SS), encoded by the <jats:italic>cag</jats:italic> pathogenicity island (<jats:italic>cag</jats:italic>‐PAI), to deliver the bacterial protein CagA into eukaryotic cells and to induce interleukin‐8 secretion. Translocated CagA is activated by tyrosine phosphorylation involving Src‐family kinases. The mechanism and structural basis for type IV protein secretion is not well understood. We describe here, by confocal laser scanning microscopy and field emission scanning electron microscopy, a novel filamentous surface organelle which is part of the <jats:italic>Hp</jats:italic> T4SS. The organelle is often located at one bacterial pole but can be induced by cell contact also along the lateral side of the bacteria. It consists of a rigid needle, covered focally or completely by HP0527 (Cag7 or CagY), a VirB10‐homologous protein. HP0527 is also clustered in the outer membrane. The VirB7‐homologous protein HP0532 is found at the base of this organelle. These observations demonstrate for the first time by microscopic techniques a complex T4SS‐associated, sheathed surface organelle reminiscent to the needle structures of bacterial type III secretion systems.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1382825896368813444","@type":"Researcher","foaf:name":[{"@value":"Manfred Rohde"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825896368813443","@type":"Researcher","foaf:name":[{"@value":"Jürgen Püls"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825896368813442","@type":"Researcher","foaf:name":[{"@value":"Renate Buhrdorf"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825896368813440","@type":"Researcher","foaf:name":[{"@value":"Wolfgang Fischer"}]},{"@id":"https://cir.nii.ac.jp/crid/1382825896368813441","@type":"Researcher","foaf:name":[{"@value":"Rainer Haas"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"0950382X"},{"@type":"EISSN","@value":"13652958"}],"prism:publicationName":[{"@value":"Molecular Microbiology"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2003-05-30","prism:volume":"49","prism:number":"1","prism:startingPage":"219","prism:endingPage":"234"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-2958.2003.03549.x"},{"@id":"https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-2958.2003.03549.x"}],"createdAt":"2003-06-19","modifiedAt":"2023-10-13","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360002218159480704","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Molecular dissection of protein–protein interactions between integrin α5β1 and the <i>Helicobacter pylori</i> Cag type <scp>IV</scp> secretion system"}]},{"@id":"https://cir.nii.ac.jp/crid/1360009142921325184","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Identification of Membrane-Bound Lytic Murein Transglycosylase A (MltA) as a Growth Factor for Francisella novicida in a Silkworm Infection Model"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283692109870464","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Native structure of a type IV secretion system core complex essential for\n            <i>Legionella</i>\n            pathogenesis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285711790863616","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Systematic analysis of phosphotyrosine antibodies recognizing single phosphorylated EPIYA-motifs in CagA of East Asian-type Helicobacter pylori strains"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567187500164864","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Systematic Analysis of Phosphotyrosine Antibodies Recognizing Single Phosphorylated EPIYA-Motifs in CagA of Western-Type Helicobacter pylori Strains"}]},{"@id":"https://cir.nii.ac.jp/crid/1360846640791108224","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Tertiary Structure-Function Analysis Reveals the Pathogenic Signaling Potentiation Mechanism of Helicobacter pylori Oncogenic Effector CagA"}]},{"@id":"https://cir.nii.ac.jp/crid/1363946392172917120","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Soluble lytic transglycosylase SLT of Francisella novicida is involved in intracellular growth and immune suppression"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204671685504","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Repressed TGF-β signaling through CagA-Smad3 interaction as pathogenic mechanisms of <i>Helicobacter pylori</i>-associated gastritis"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001206454753792","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Structure and function of the Type IV secretion systems of bacterial pathogens"},{"@language":"ja","@value":"病原細菌のＩＶ型分泌系"},{"@value":"病原細菌の4型分泌系"},{"@language":"ja-Kana","@value":"ビョウゲン サイキン ノ 4ガタ 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