{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363388843390289920.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1113/jphysiol.2001.012606"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1113%2Fjphysiol.2001.012606"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/pdf/10.1113/jphysiol.2001.012606"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/full-xml/10.1113/jphysiol.2001.012606"}},{"identifier":{"@type":"URI","@value":"https://physoc.onlinelibrary.wiley.com/doi/pdf/10.1113/jphysiol.2001.012606"}}],"dc:title":[{"@value":"Coupling of vasopressin‐induced intracellular Ca<sup>2+</sup> mobilization and apical exocytosis in perfused rat kidney collecting duct"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>Arginine vasopressin (AVP) regulates the osmotic water permeability of the kidney collecting duct by inducing exocytotic insertion of aquaporin‐2 into apical membrane. The coupling between AVP‐induced intracellular Ca<jats:sup>2+</jats:sup> mobilization and apical exocytosis was investigated in isolated perfused rat inner medullary collecting duct (IMCD) segments using confocal fluorescence microscopy. Changes of [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub> in IMCD cells were measured with fluo‐4. A novel confocal imaging technique using a styryl dye, FM1‐43, was developed to monitor real‐time exocytosis induced by arginine vasopressin. AVP (0.1 n<jats:sc>m</jats:sc>) triggered a rapid increase of [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub> in IMCD cells, followed by sustained oscillations. Ratiometric measurement of [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub> confirmed that the observed [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub> oscillation was a primary event and was not secondary to changes in cell volume. The frequencies of [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub> oscillations in each IMCD cell were independent and time variant. 1‐Deamino‐8‐<jats:sc>d</jats:sc>‐arginine vasopressin (a V<jats:sub>2</jats:sub> receptor agonist, 0.1 n<jats:sc>m</jats:sc>) simulated the effects of AVP by triggering [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub> oscillations. In the absence of extracellular Ca<jats:sup>2+</jats:sup>, ryanodine (0.1 m<jats:sc>m</jats:sc>) inhibited AVP‐induced Ca<jats:sup>2+</jats:sup> mobilization. AVP (0.1 n<jats:sc>m</jats:sc>) triggered accumulative apical exocytosis in IMCD cells within 20 s after application. Pre‐incubating the IMCD with an intracellular Ca<jats:sup>2+</jats:sup> chelator, BAPTA, prevented AVP‐induced intracellular Ca<jats:sup>2+</jats:sup> mobilization, apical exocytosis, and increase of osmotic water permeability. These results indicate that AVP, via the V<jats:sub>2</jats:sub> receptor, triggers a calcium signalling cascade observed as [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub> oscillations in the IMCD and that intracellular Ca<jats:sup>2+</jats:sup> mobilization is required for exocytotic insertion of aquaporin‐2.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1383388843390289920","@type":"Researcher","foaf:name":[{"@value":"Kay‐Pong Yip"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00223751"},{"@type":"EISSN","@value":"14697793"}],"prism:publicationName":[{"@value":"The Journal of Physiology"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2002-02","prism:volume":"538","prism:number":"3","prism:startingPage":"891","prism:endingPage":"899"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1113%2Fjphysiol.2001.012606"},{"@id":"https://onlinelibrary.wiley.com/doi/pdf/10.1113/jphysiol.2001.012606"},{"@id":"https://onlinelibrary.wiley.com/doi/full-xml/10.1113/jphysiol.2001.012606"},{"@id":"https://physoc.onlinelibrary.wiley.com/doi/pdf/10.1113/jphysiol.2001.012606"}],"createdAt":"2002-07-27","modifiedAt":"2023-09-04","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1361412896231235968","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"CRISPR-Cas9/phosphoproteomics identifies multiple noncanonical targets of myosin light chain kinase"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204587082880","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Arg-vasopressin Facilitates Calcium Channel Currents in Osteoblasts"}]},{"@id":"https://cir.nii.ac.jp/crid/2051433317029673984","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Activation of AQP2 water channels without vasopressin : therapeutic strategies for congenital nephrogenic diabetes insipidus"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1113/jphysiol.2001.012606"},{"@type":"CROSSREF","@value":"10.2209/tdcpublication.55.241_references_DOI_5Uc5BIBOojM0gqbTxbf63WPbWBh"},{"@type":"CROSSREF","@value":"10.1007/s10157-018-1544-8_references_DOI_5Uc5BIBOojM0gqbTxbf63WPbWBh"},{"@type":"CROSSREF","@value":"10.1152/ajprenal.00431.2019_references_DOI_5Uc5BIBOojM0gqbTxbf63WPbWBh"}]}