{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363388843569103232.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1161/01.str.23.8.1118"}},{"identifier":{"@type":"URI","@value":"https://www.ahajournals.org/doi/pdf/10.1161/01.STR.23.8.1118"}}],"dc:title":[{"@value":"Endothelium-derived nitric oxide synthase inhibition. Effects on cerebral blood flow, pial artery diameter, and vascular morphology in rats."}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>We determined the effects of inhibiting the production of cerebral endothelium-derived nitric oxide on pial artery diameter, cortical blood flow, and vascular morphology.</jats:p>\n          <jats:p>An inhibitor of endothelium-derived nitric oxide synthesis, NG-nitro-L-arginine methyl ester hydrochloride (L-NAME), or an equivalent volume of 0.9% saline was infused into rats intra-arterially in a retrograde fashion via the right external carotid artery at a rate of 3 mg/kg/min to a total dose of 190 mg/kg or intravenously at 1 mg/kg/min to a total dose of 15 mg/kg. Large pial arteries were continuously visualized through an operating microscope, and cortical cerebral blood flow was monitored by laser-Doppler flowmetry. To localize areas of morphological interest, the protein tracer horseradish peroxidase was injected 15 minutes before termination of the L-NAME infusion and the rats were perfusion-fixed 15 minutes later for light and electron microscopic analysis.</jats:p>\n          <jats:p>Infusion of L-NAME significantly raised arterial blood pressure at both doses (for 190 mg/kg, from 103.2 +/- 3.4 to 135 +/- 3.4 mm Hg; for 15 mg/kg, from 125 +/- 2.8 to 144.4 +/- 4.0 mm Hg). Pial arteries constricted within 10 minutes after the start of the intracarotid infusion to 40% of the preinfusion diameter, while cortical cerebral blood flow decreased to an average of 72.5% of that at baseline. Morphological abnormalities in the experimental rats included microvascular stasis and focal areas of blood-brain barrier disruption to protein. Ultrastructural examination of cortical leaky sites revealed constricted arterioles with many endothelial pinocytotic vesicles and microvilli.</jats:p>\n          <jats:p>These observations suggest that inhibition of endothelium-derived nitric oxide synthesis affects the relation between cerebral arterial diameter and cerebral blood flow and can lead to subtle cerebral vascular pathological changes consistent with focal brain ischemia.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1383388843569103233","@type":"Researcher","foaf:name":[{"@value":"R Prado"}],"jpcoar:affiliationName":[{"@value":"Cerebral Vascular Disease Research Center, University of Miami School of Medicine, FL 33101."}]},{"@id":"https://cir.nii.ac.jp/crid/1383388843569103235","@type":"Researcher","foaf:name":[{"@value":"B D Watson"}],"jpcoar:affiliationName":[{"@value":"Cerebral Vascular Disease Research Center, University of Miami School of Medicine, FL 33101."}]},{"@id":"https://cir.nii.ac.jp/crid/1383388843569103234","@type":"Researcher","foaf:name":[{"@value":"J Kuluz"}],"jpcoar:affiliationName":[{"@value":"Cerebral Vascular Disease Research Center, University of Miami School of Medicine, FL 33101."}]},{"@id":"https://cir.nii.ac.jp/crid/1383388843569103232","@type":"Researcher","foaf:name":[{"@value":"W D Dietrich"}],"jpcoar:affiliationName":[{"@value":"Cerebral Vascular Disease Research Center, University of Miami School of Medicine, FL 33101."}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00392499"},{"@type":"EISSN","@value":"15244628"},{"@type":"PISSN","@value":"https://id.crossref.org/issn/00392499"}],"prism:publicationName":[{"@value":"Stroke"}],"dc:publisher":[{"@value":"Ovid Technologies (Wolters Kluwer Health)"}],"prism:publicationDate":"1992-08","prism:volume":"23","prism:number":"8","prism:startingPage":"1118","prism:endingPage":"1123"},"reviewed":"false","url":[{"@id":"https://www.ahajournals.org/doi/pdf/10.1161/01.STR.23.8.1118"}],"createdAt":"2011-06-17","modifiedAt":"2024-05-12","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1390001204287624576","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Cerebral Vasodilators"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282680026214528","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Endogenous Nitric Oxide Synthesis Differentially Modulates Pressure-Flow and Pressure-Conductance Relationships in the Internal and External Carotid Artery Circulations of the Rat"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1161/01.str.23.8.1118"},{"@type":"CROSSREF","@value":"10.1254/jjp.76.349_references_DOI_JlpEkvH5MPi76yEptX1eCOk3l6F"},{"@type":"CROSSREF","@value":"10.2176/nmc.42.527_references_DOI_JlpEkvH5MPi76yEptX1eCOk3l6F"}]}