{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363388843650247296.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1073/pnas.84.2.344"}},{"identifier":{"@type":"URI","@value":"https://pnas.org/doi/pdf/10.1073/pnas.84.2.344"}},{"identifier":{"@type":"NAID","@value":"80003242793"}}],"dc:title":[{"@value":"DNA base changes and alkylation following in vivo exposure of Escherichia coli to N-methyl-N-nitrosourea or N-ethyl-N-nitrosourea."}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>Dideoxy chain-termination DNA sequencing was used to determine the specific DNA base changes induced after in vivo exposure of Escherichia coli to N-methyl-N-nitrosourea (MNU) and N-ethyl-N-nitrosourea (ENU) using the xanthine guanine phosphoribosyltransferase (gpt) gene as the genetic target. The resultant mutation spectra were compared with the levels of O6-alkylguanine and O4-alkylthymidine in genomic DNA immediately after exposure. All (39/39) of the MNU-induced mutations were G X C----A X T transitions. In contrast, 24/33 point mutations isolated following ENU treatment were G X C----A X T transitions, 7/33 were A X T----G X C transitions, 1/33 was a G X C----C X G transversion, and 1/33 was an A X T----C X G transversion. Three large insertions, probably of spontaneous origin, were also isolated. O4-alkylthymidine/O6-alkylguanine ratios were 0.014 for MNU and 0.28 for ENU. These data suggest that the difference in the mutation spectrum of MNU versus ENU may be attributed, in part, to the different ratio of O6-alkylguanine versus O4-alkylthymidine produced in the DNA. Of the G X C----A X T transitions, 82% of the MNU- and 71% of the ENU-induced mutations occurred at the middle guanine of the sequence 5'-GG(A or T)-3'.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1580572701684868992","@type":"Researcher","foaf:name":[{"@value":"K K Richardson"}]},{"@id":"https://cir.nii.ac.jp/crid/1380016865966467329","@type":"Researcher","foaf:name":[{"@value":"F C Richardson"}]},{"@id":"https://cir.nii.ac.jp/crid/1380016865966467328","@type":"Researcher","foaf:name":[{"@value":"R M Crosby"}]},{"@id":"https://cir.nii.ac.jp/crid/1380016865966467332","@type":"Researcher","foaf:name":[{"@value":"J A Swenberg"}]},{"@id":"https://cir.nii.ac.jp/crid/1380016865966467331","@type":"Researcher","foaf:name":[{"@value":"T R Skopek"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00278424"},{"@type":"EISSN","@value":"10916490"}],"prism:publicationName":[{"@value":"Proceedings of the National Academy of Sciences"}],"dc:publisher":[{"@value":"Proceedings of the National Academy of Sciences"}],"prism:publicationDate":"1987-01","prism:volume":"84","prism:number":"2","prism:startingPage":"344","prism:endingPage":"348"},"reviewed":"false","url":[{"@id":"https://pnas.org/doi/pdf/10.1073/pnas.84.2.344"}],"createdAt":"2006-05-31","modifiedAt":"2022-04-13","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050845760762760832","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Mutation assay using single-molecule real-time (SMRT™) sequencing technology"},{"@value":"Mutation assay using single-molecule real-time (SMRT) sequencing technology"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679699707392","@type":"Article","relationType":["isReferencedBy","isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Use of N-methyl-N-nitrosourea treatment of fertilized egg cells for saturation mutagenesis of rice"}]},{"@id":"https://cir.nii.ac.jp/crid/1520853833846423040","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@value":"Comparison of mutation spectra induced by ethylating agents in DNA-alkyltransferase-deficient mutants of E.coli"},{"@language":"ja-Kana","@value":"Comparison of mutation spectra induced by ethylating agents in DNA alkyltransferase deficient mutants of E coli"}]},{"@id":"https://cir.nii.ac.jp/crid/1572543024541960704","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Different Mutation Frequencies and Spectra among Organs by N-Melthyl-N-nitrosourea in rpsL (strA) Transgenic Mice"}]},{"@id":"https://cir.nii.ac.jp/crid/1574231874400110336","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Demonstration of ras and p.53 Gene Mutations in Carcinomas in the Forestomach and Intestine and Soft Tissue Sarcomas Induced by N-Methyl-N-nitrosourea in the Rat"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1073/pnas.84.2.344"},{"@type":"CIA","@value":"80003242793"},{"@type":"CROSSREF","@value":"10.1186/s41021-015-0017-5_references_DOI_3iNHer41pE0KanbPk1BhumLGrI3"},{"@type":"CROSSREF","@value":"10.1270/jsbbs.60.475_references_DOI_3iNHer41pE0KanbPk1BhumLGrI3"}]}