{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363388844282017024.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1074/jbc.m200396200"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0021925820855124?httpAccept=text/xml"}},{"identifier":{"@type":"URI","@value":"https://api.elsevier.com/content/article/PII:S0021925820855124?httpAccept=text/plain"}},{"identifier":{"@type":"URI","@value":"https://syndication.highwire.org/content/doi/10.1074/jbc.M200396200"}},{"identifier":{"@type":"PMID","@value":"11821431"}},{"identifier":{"@type":"NAID","@value":"80015273437"}}],"dc:title":[{"@value":"Oversulfated Chondroitin/Dermatan Sulfates Containing GlcAβ1/IdoAα1–3GalNAc(4,6-O-disulfate) Interact with L- and P-selectin and Chemokines"}],"description":[{"notation":[{"@value":"We previously reported that versican, a large chondroitin/dermatan sulfate (CS/DS) proteoglycan, interacts through its CS/DS chains with adhesion molecules L- and P-selectin and CD44, as well as chemokines. Here, we have characterized these interactions further. Using a metabolic inhibitor of sulfation, sodium chlorate, we show that the interactions of the CS/DS chains of versican with L- and P-selectin and chemokines are sulfation-dependent but the interaction with CD44 is sulfation-independent. Consistently, versican's binding to L- and P-selectin and chemokines is specifically inhibited by oversulfated CS/DS chains containing GlcAbeta1-3GalNAc(4,6-O-disulfate) or IdoAalpha1-3GalNAc(4,6-O-disulfate), but its binding to CD44 is inhibited by all the CS/DS chains, including low-sulfated and unsulfated ones. Affinity and kinetic analyses using surface plasmon resonance revealed that the oversulfated CS/DS chains containing GlcAbeta1/IdoAalpha1-3GalNAc(4,6-O-disulfate) bind directly to selectins and chemokines with high affinity (K(d) 21.1 to 293 nm). In addition, a tetrasaccharide fragment of repeating GlcAbeta1-3GalNAc(4,6-O-disulfate) units directly interacts with L- and P-selectin and chemokines and oversulfated CS/DS chains containing GlcAbeta1/IdoAalpha1-3GalNAc(4,6-O-disulfate) inhibit chemokine-induced Ca(2+) mobilization. Taken together, our results show that oversulfated CS/DS chains containing GlcAbeta1/IdoAalpha1-3GalNAc(4,6-O-disulfate) are recognized by L- and P-selectin and chemokines, and imply that these chains are important in selectin- and/or chemokine-mediated cellular responses."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1383388844282017030","@type":"Researcher","foaf:name":[{"@value":"Hiroto Kawashima"}]},{"@id":"https://cir.nii.ac.jp/crid/1383388844282017025","@type":"Researcher","foaf:name":[{"@value":"Kazuyuki Atarashi"}]},{"@id":"https://cir.nii.ac.jp/crid/1383388844282017027","@type":"Researcher","foaf:name":[{"@value":"Mayumi Hirose"}]},{"@id":"https://cir.nii.ac.jp/crid/1383388844282017026","@type":"Researcher","foaf:name":[{"@value":"Jun Hirose"}]},{"@id":"https://cir.nii.ac.jp/crid/1383388844282017024","@type":"Researcher","foaf:name":[{"@value":"Shuhei Yamada"}]},{"@id":"https://cir.nii.ac.jp/crid/1383388844282017028","@type":"Researcher","foaf:name":[{"@value":"Kazuyuki Sugahara"}]},{"@id":"https://cir.nii.ac.jp/crid/1383388844282017029","@type":"Researcher","foaf:name":[{"@value":"Masayuki Miyasaka"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00219258"},{"@type":"PISSN","@value":"http://id.crossref.org/issn/00219258"}],"prism:publicationName":[{"@value":"Journal of Biological Chemistry"}],"dc:publisher":[{"@value":"Elsevier BV"}],"prism:publicationDate":"2002-04","prism:volume":"277","prism:number":"15","prism:startingPage":"12921","prism:endingPage":"12930"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["https://www.elsevier.com/tdm/userlicense/1.0/","http://creativecommons.org/licenses/by/4.0/"],"url":[{"@id":"https://api.elsevier.com/content/article/PII:S0021925820855124?httpAccept=text/xml"},{"@id":"https://api.elsevier.com/content/article/PII:S0021925820855124?httpAccept=text/plain"},{"@id":"https://syndication.highwire.org/content/doi/10.1074/jbc.M200396200"}],"createdAt":"2002-07-26","modifiedAt":"2022-01-05","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Base%20Sequence","dc:title":"Base Sequence"},{"@id":"https://cir.nii.ac.jp/all?q=Chondroitin%20Sulfates","dc:title":"Chondroitin Sulfates"},{"@id":"https://cir.nii.ac.jp/all?q=Dermatan%20Sulfate","dc:title":"Dermatan Sulfate"},{"@id":"https://cir.nii.ac.jp/all?q=Oligosaccharides","dc:title":"Oligosaccharides"},{"@id":"https://cir.nii.ac.jp/all?q=Kinetics","dc:title":"Kinetics"},{"@id":"https://cir.nii.ac.jp/all?q=P-Selectin","dc:title":"P-Selectin"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=L-Selectin","dc:title":"L-Selectin"},{"@id":"https://cir.nii.ac.jp/all?q=DNA%20Primers","dc:title":"DNA Primers"},{"@id":"https://cir.nii.ac.jp/all?q=Protein%20Binding","dc:title":"Protein Binding"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050022853110732672","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Structural and functional changes of sulfated glycosaminoglycans in Xenopus laevis during embryogenesis"}]},{"@id":"https://cir.nii.ac.jp/crid/1050025031478175872","@type":"Article","resourceType":"学術雑誌論文(journal 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