{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363388844474595072.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1242/jeb.00178"}},{"identifier":{"@type":"URI","@value":"http://journals.biologists.com/jeb/article-pdf/206/6/979/1246565/979.pdf"}},{"identifier":{"@type":"NAID","@value":"30002329557"}}],"dc:title":[{"@value":"Putative involvement of crustacean hyperglycemic hormone isoforms in the neuroendocrine mediation of osmoregulation in the crayfish<i>Astacus leptodactylus</i>"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>SUMMARY</jats:title><jats:p>This study investigates the involvement of eyestalk neuroendocrine factors on osmoregulation in the crayfish Astacus leptodactylus maintained in freshwater. Eyestalk removal was followed by a significant decrease in hemolymph osmolality and Na+ concentration and by a 50% increase in mass after one molting cycle. Several neurohormones have been isolated from the sinus gland through high-performance liquid chromatography (HPLC), and different crustacean hyperglycemic hormone (CHH)-related peptides, including stereoisomers (L-CHH and D-Phe3 CHH), have been identified by direct enzyme-linked immunosorbent assay (ELISA). A glucose quantification bioassay demonstrated a strong hyperglycemic activity following injection of the immunoreactive chromatographic fractions and showed that the D-Phe3 CHH was the most efficient. Destalked crayfish were then injected with purified CHH HPLC fractions. The D-Phe3 CHH fraction significantly increased the hemolymph osmolality and Na+ content 24 h after injection. Two other CHH-related peptides caused a smaller increase in Na+ concentration. No significant variation was observed in hemolymph Cl- concentration following injection of any of the CHH isoforms. These results constitute the first observation of the effects of a CHH isoform, specifically the D-Phe3 CHH, on osmoregulatory parameters in a freshwater crustacean. The effects of eyestalk ablation and CHH injection on osmoregulation and the identification of different CHH-related peptides and isoforms in crustaceans are discussed.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1383388844474595076","@type":"Researcher","foaf:name":[{"@value":"Laetitia Serrano"}],"jpcoar:affiliationName":[{"@value":"Laboratoire Génome, Populations, Interactions, Adaptation, UMR 5000, Equipe Adaptation Ecophysiologique et Ontogenèse,Université Montpellier II, Place E. Bataillon, CP 092, 34095 Montpellier Cédex 05, France"}]},{"@id":"https://cir.nii.ac.jp/crid/1383388844474595072","@type":"Researcher","foaf:name":[{"@value":"Gaëlle Blanvillain"}],"jpcoar:affiliationName":[{"@value":"Laboratoire Génome, Populations, Interactions, Adaptation, UMR 5000, Equipe Adaptation Ecophysiologique et Ontogenèse,Université Montpellier II, Place E. Bataillon, CP 092, 34095 Montpellier Cédex 05, France"}]},{"@id":"https://cir.nii.ac.jp/crid/1383388844474595078","@type":"Researcher","foaf:name":[{"@value":"Daniel Soyez"}],"jpcoar:affiliationName":[{"@value":"Groupe Biogenèse des Peptides Isomères, UMR CNRS Physiologie et Physiopathologie, Université Paris VI, CC 256, 7 Quai Saint-Bernard,75252 Paris Cédex 05, France"}]},{"@id":"https://cir.nii.ac.jp/crid/1383388844474595074","@type":"Researcher","foaf:name":[{"@value":"Guy Charmantier"}],"jpcoar:affiliationName":[{"@value":"Laboratoire Génome, Populations, Interactions, Adaptation, UMR 5000, Equipe Adaptation Ecophysiologique et Ontogenèse,Université Montpellier II, Place E. Bataillon, CP 092, 34095 Montpellier Cédex 05, France"}]},{"@id":"https://cir.nii.ac.jp/crid/1383388844474595073","@type":"Researcher","foaf:name":[{"@value":"Evelyse Grousset"}],"jpcoar:affiliationName":[{"@value":"Laboratoire Génome, Populations, Interactions, Adaptation, UMR 5000, Equipe Adaptation Ecophysiologique et Ontogenèse,Université Montpellier II, Place E. Bataillon, CP 092, 34095 Montpellier Cédex 05, France"}]},{"@id":"https://cir.nii.ac.jp/crid/1383388844474595075","@type":"Researcher","foaf:name":[{"@value":"Fabien Aujoulat"}],"jpcoar:affiliationName":[{"@value":"Laboratoire Génome, Populations, Interactions, Adaptation, UMR 5000, Equipe Adaptation Ecophysiologique et Ontogenèse,Université Montpellier II, Place E. Bataillon, CP 092, 34095 Montpellier Cédex 05, France"}]},{"@id":"https://cir.nii.ac.jp/crid/1383388844474595077","@type":"Researcher","foaf:name":[{"@value":"Céline Spanings-Pierrot"}],"jpcoar:affiliationName":[{"@value":"Laboratoire Génome, Populations, Interactions, Adaptation, UMR 5000, Equipe Adaptation Ecophysiologique et Ontogenèse,Université Montpellier II, Place E. Bataillon, CP 092, 34095 Montpellier Cédex 05, France"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"14779145"},{"@type":"PISSN","@value":"00220949"}],"prism:publicationName":[{"@value":"Journal of Experimental Biology"}],"dc:publisher":[{"@value":"The Company of Biologists"}],"prism:publicationDate":"2003-03-15","prism:volume":"206","prism:number":"6","prism:startingPage":"979","prism:endingPage":"988"},"reviewed":"false","url":[{"@id":"http://journals.biologists.com/jeb/article-pdf/206/6/979/1246565/979.pdf"}],"createdAt":"2003-02-11","modifiedAt":"2021-06-04","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1523106604771947392","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Molecular Cloning of Two Structure Variants of Crustacean Hyperglycemic Hormone (CHH) from the Swimming Crab (Portunus trituberculatus), and Their Gene Expression During Molting and Ovarian Development"}]},{"@id":"https://cir.nii.ac.jp/crid/1570572700297726464","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Purification of molt-inhibiting hormone-like peptides with hyperglycemic activity from the eyestalks of the crab Scylla serrata"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1242/jeb.00178"},{"@type":"CIA","@value":"30002329557"},{"@type":"CROSSREF","@value":"10.2108/zs140053_references_DOI_JMoEoXpJUr3T0feqpKkoVBAeeQv"}]}