{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363388845186944896.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1002/rcm.3252"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Frcm.3252"}},{"identifier":{"@type":"URI","@value":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/pdf/10.1002/rcm.3252"}}],"dc:title":[{"@value":"Optimisation of derivatisation procedures for the determination of\n                    <i>δ</i>\n                    <sup>13</sup>\n                    C values of amino acids by gas chromatography/combustion/isotope ratio mass spectrometry"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title>\n                  <jats:p>\n                    Compound‐specific stable carbon isotope analysis of amino acids by gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) is a highly selective and sensitive method for probing the biosynthetic/diagenetic pathways, pool size and turnover rates of proteins, previously intractable to bulk isotope analyses. However, amino acids are polyfunctional, non‐volatile compounds which require derivatisation prior to GC analysis. While a wide range of derivatives exist for the GC analysis of amino acids only a handful have been utilised for their GC/C/IRMS analysis. Significantly, none of those derivatives currently employed appear completely satisfactory and a thorough assessment of their relative utility is lacking. Seven derivatives (three previously reported and four novel) for obtaining\n                    <jats:italic>δ</jats:italic>\n                    <jats:sup>13</jats:sup>\n                    C values of amino acids via GC/C/IRMS analysis were compared. More specifically, standard mixtures of 15 protein amino acids were converted into\n                    <jats:italic>N</jats:italic>\n                    ‐acetylmethyl (NACME) esters,\n                    <jats:italic>N</jats:italic>\n                    ‐acetyl\n                    <jats:italic>n</jats:italic>\n                    ‐propyl (NANP) esters,\n                    <jats:italic>N</jats:italic>\n                    ‐acetyl\n                    <jats:italic>i</jats:italic>\n                    ‐propyl (NAIP) esters,\n                    <jats:italic>N</jats:italic>\n                    ‐trifluoroacetyl‐\n                    <jats:italic>i</jats:italic>\n                    ‐propyl (TFA‐IP) esters,\n                    <jats:italic>N</jats:italic>\n                    ‐pivaloyl methyl (NPME) esters,\n                    <jats:italic>N</jats:italic>\n                    ‐pivaloyl\n                    <jats:italic>n</jats:italic>\n                    ‐propyl (NPNP) esters and\n                    <jats:italic>N</jats:italic>\n                    ‐pivaloyl\n                    <jats:italic>i</jats:italic>\n                    ‐propyl (NPIP) esters. Each derivative was assessed with respect to its applicability to carbon isotope determinations of all the common\n                    <jats:italic>α</jats:italic>\n                    ‐amino acids, reaction yield, chromatographic resolution, stability, analyte‐to‐derivative carbon ratio, kinetic isotope effects and errors associated with their carbon isotope determinations. The NACME derivative was concluded to be the preferred derivative mainly due to the highest analyte‐to‐derivative carbon ratio being achieved, resulting in the lowest analytical errors for amino acid\n                    <jats:italic>δ</jats:italic>\n                    <jats:sup>13</jats:sup>\n                    C value determinations, ranging from ±0.6‰ for phenylalanine, leucine and isoleucine to ±1.1‰ for serine and glycine. Copyright © 2007 John Wiley & Sons, Ltd.\n                  </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1383388845186944897","@type":"Researcher","foaf:name":[{"@value":"Lorna T. Corr"}]},{"@id":"https://cir.nii.ac.jp/crid/1383388845186944898","@type":"Researcher","foaf:name":[{"@value":"Robert Berstan"}]},{"@id":"https://cir.nii.ac.jp/crid/1383388845186944896","@type":"Researcher","foaf:name":[{"@value":"Richard P. Evershed"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"09514198"},{"@type":"EISSN","@value":"10970231"}],"prism:publicationName":[{"@value":"Rapid Communications in Mass Spectrometry"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2007-11-27","prism:volume":"21","prism:number":"23","prism:startingPage":"3759","prism:endingPage":"3771"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Frcm.3252"},{"@id":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/pdf/10.1002/rcm.3252"}],"createdAt":"2007-11-07","modifiedAt":"2025-10-26","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360009142672968192","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A method for stable carbon isotope measurement of underivatized individual amino acids by multi‐dimensional high‐performance liquid chromatography and elemental analyzer/isotope ratio mass spectrometry"}]},{"@id":"https://cir.nii.ac.jp/crid/1360021390554195072","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Application of a porous graphitic carbon column to carbon and nitrogen isotope analysis of underivatized individual amino acids using high‐performance liquid chromatography coupled with elemental analyzer/isotope ratio mass spectrometry"}]},{"@id":"https://cir.nii.ac.jp/crid/1360298345518042112","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Integrative assessment of amino acid nitrogen isotopic composition in biological tissue samples determined by <scp>GC</scp>/C/<scp>IRMS</scp>, <scp>LC</scp> × <scp>EA</scp>/<scp>IRMS</scp>, and <scp>LC</scp> × <scp>GC</scp>/C/<scp>IRMS</scp>"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565164612928640","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Isolation and desalting with cation‐exchange chromatography for compound‐specific nitrogen isotope analysis of amino acids: application to biogeochemical samples"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567182155605120","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Isolation of underivatized amino acids by ion-pair high performance liquid chromatography for precise measurement of nitrogen isotopic composition of amino acids: Development of comprehensive LC × GC/C/IRMS method"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567182198308736","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"An overview of methods used for the detection of aquatic resource consumption by humans: Compound-specific delta N-15 analysis of amino acids in archaeological materials"}]},{"@id":"https://cir.nii.ac.jp/crid/2050307417121637504","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A new insight into isotopic fractionation associated with decarboxylation in organisms : implications for amino acid isotope approaches in biogeoscience"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1002/rcm.3252"},{"@type":"CROSSREF","@value":"10.1002/rcm.8885_references_DOI_Bb2Ke1RvUnJa5boYsVsaLxGmQBx"},{"@type":"CROSSREF","@value":"10.1002/lom3.10502_references_DOI_Bb2Ke1RvUnJa5boYsVsaLxGmQBx"},{"@type":"CROSSREF","@value":"10.1002/rcm.9602_references_DOI_Bb2Ke1RvUnJa5boYsVsaLxGmQBx"},{"@type":"CROSSREF","@value":"10.1186/s40645-020-00364-w_references_DOI_Bb2Ke1RvUnJa5boYsVsaLxGmQBx"},{"@type":"CROSSREF","@value":"10.1002/rcm.4651_references_DOI_Bb2Ke1RvUnJa5boYsVsaLxGmQBx"},{"@type":"CROSSREF","@value":"10.1016/j.ijms.2014.11.012_references_DOI_Bb2Ke1RvUnJa5boYsVsaLxGmQBx"},{"@type":"CROSSREF","@value":"10.1016/j.jasrep.2015.11.025_references_DOI_Bb2Ke1RvUnJa5boYsVsaLxGmQBx"}]}