{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363388845529838976.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1073/pnas.032658999"}},{"identifier":{"@type":"URI","@value":"https://pnas.org/doi/pdf/10.1073/pnas.032658999"}},{"identifier":{"@type":"NAID","@value":"80015335553"}}],"dc:title":[{"@value":"Studies on the nonmevalonate terpene biosynthetic pathway: Metabolic role of IspH (LytB) protein"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>\n            Isopentenyl diphosphate and dimethylallyl diphosphate serve as the universal precursors for the biosynthesis of terpenes. Although their biosynthesis by means of mevalonate has been studied in detail, a second biosynthetic pathway for their formation by means of 1-deoxy-\n            <jats:sc>d</jats:sc>\n            -xylulose 5-phosphate has been discovered only recently in plants and certain eubacteria. Earlier\n            <jats:italic>in vivo</jats:italic>\n            experiments with recombinant\n            <jats:italic>Escherichia coli</jats:italic>\n            strains showed that exogenous 1-deoxy-\n            <jats:sc>d</jats:sc>\n            -xylulose can be converted into 1-hydroxy-2-methyl-2-(\n            <jats:italic>E</jats:italic>\n            )-butenyl 4-diphosphate by the consecutive action of enzymes specified by the\n            <jats:italic>xylB</jats:italic>\n            and\n            <jats:italic>ispCDEFG</jats:italic>\n            genes. This article describes the transformation of exogenous [U-\n            <jats:sup>13</jats:sup>\n            C\n            <jats:sub>5</jats:sub>\n            ]1-deoxy-\n            <jats:sc>d</jats:sc>\n            -xylulose into a 5:1 mixture of [U-\n            <jats:sup>13</jats:sup>\n            C\n            <jats:sub>5</jats:sub>\n            ]isopentenyl diphosphate and [U-\n            <jats:sup>13</jats:sup>\n            C\n            <jats:sub>5</jats:sub>\n            ]dimethylallyl diphosphate by an\n            <jats:italic>E. coli</jats:italic>\n            strain engineered for the expression of the\n            <jats:italic>ispH (lytB)</jats:italic>\n            gene in addition to recombinant\n            <jats:italic>xylB</jats:italic>\n            and\n            <jats:italic>ispCDEFG</jats:italic>\n            genes.\n          </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380576197203260162","@type":"Researcher","foaf:name":[{"@value":"Felix Rohdich"}],"jpcoar:affiliationName":[{"@value":"Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstraβe 4, D-85747 Garching, Germany; and Laboratorium für Organische Chemie, Eidgenössische Technische Hochschule-Hönggerberg Chemisches Institut, H307, CH-8093 Zürich, Switzerland"}]},{"@id":"https://cir.nii.ac.jp/crid/1380576197203260037","@type":"Researcher","foaf:name":[{"@value":"Stefan Hecht"}],"jpcoar:affiliationName":[{"@value":"Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstraβe 4, D-85747 Garching, Germany; and Laboratorium für Organische Chemie, Eidgenössische Technische Hochschule-Hönggerberg Chemisches Institut, H307, CH-8093 Zürich, Switzerland"}]},{"@id":"https://cir.nii.ac.jp/crid/1380576197203260161","@type":"Researcher","foaf:name":[{"@value":"Katrin Gärtner"}],"jpcoar:affiliationName":[{"@value":"Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstraβe 4, D-85747 Garching, Germany; and Laboratorium für Organische Chemie, Eidgenössische Technische Hochschule-Hönggerberg Chemisches Institut, H307, CH-8093 Zürich, Switzerland"}]},{"@id":"https://cir.nii.ac.jp/crid/1380576197203260036","@type":"Researcher","foaf:name":[{"@value":"Petra Adam"}],"jpcoar:affiliationName":[{"@value":"Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstraβe 4, D-85747 Garching, Germany; and Laboratorium für Organische Chemie, Eidgenössische Technische Hochschule-Hönggerberg Chemisches Institut, H307, CH-8093 Zürich, Switzerland"}]},{"@id":"https://cir.nii.ac.jp/crid/1380576197203260033","@type":"Researcher","foaf:name":[{"@value":"Cornelia Krieger"}],"jpcoar:affiliationName":[{"@value":"Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstraβe 4, D-85747 Garching, Germany; and Laboratorium für Organische Chemie, Eidgenössische Technische Hochschule-Hönggerberg Chemisches Institut, H307, CH-8093 Zürich, Switzerland"}]},{"@id":"https://cir.nii.ac.jp/crid/1380576197203260160","@type":"Researcher","foaf:name":[{"@value":"Sabine Amslinger"}],"jpcoar:affiliationName":[{"@value":"Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstraβe 4, D-85747 Garching, Germany; and Laboratorium für Organische Chemie, Eidgenössische Technische Hochschule-Hönggerberg Chemisches Institut, H307, CH-8093 Zürich, Switzerland"}]},{"@id":"https://cir.nii.ac.jp/crid/1380576197203260034","@type":"Researcher","foaf:name":[{"@value":"Duilio Arigoni"}],"jpcoar:affiliationName":[{"@value":"Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstraβe 4, D-85747 Garching, Germany; and Laboratorium für Organische Chemie, Eidgenössische Technische Hochschule-Hönggerberg Chemisches Institut, H307, CH-8093 Zürich, Switzerland"}]},{"@id":"https://cir.nii.ac.jp/crid/1380576197203260032","@type":"Researcher","foaf:name":[{"@value":"Adelbert Bacher"}],"jpcoar:affiliationName":[{"@value":"Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstraβe 4, D-85747 Garching, Germany; and Laboratorium für Organische Chemie, Eidgenössische Technische Hochschule-Hönggerberg Chemisches Institut, H307, CH-8093 Zürich, Switzerland"}]},{"@id":"https://cir.nii.ac.jp/crid/1380576197203260035","@type":"Researcher","foaf:name":[{"@value":"Wolfgang Eisenreich"}],"jpcoar:affiliationName":[{"@value":"Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstraβe 4, D-85747 Garching, Germany; and Laboratorium für Organische Chemie, Eidgenössische Technische Hochschule-Hönggerberg Chemisches Institut, H307, CH-8093 Zürich, Switzerland"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00278424"},{"@type":"EISSN","@value":"10916490"}],"prism:publicationName":[{"@value":"Proceedings of the National Academy of Sciences"}],"dc:publisher":[{"@value":"Proceedings of the National Academy of Sciences"}],"prism:publicationDate":"2002-01-29","prism:volume":"99","prism:number":"3","prism:startingPage":"1158","prism:endingPage":"1163"},"reviewed":"false","url":[{"@id":"https://pnas.org/doi/pdf/10.1073/pnas.032658999"}],"createdAt":"2002-07-26","modifiedAt":"2022-06-07","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360009142602178560","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Determination of human γδ T cell–mediated cytotoxicity using a non-radioactive assay system"}]},{"@id":"https://cir.nii.ac.jp/crid/1360306904404378880","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Five unaddressed questions about cytokinin biosynthesis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565165240756224","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Zoledronic acid-induced expansion of γδ T cells from early-stage breast cancer patients: effect of IL-18 on helper NK cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567183512606336","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Convergent strategies in biosynthesis"}]},{"@id":"https://cir.nii.ac.jp/crid/1361699995671405568","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Molecular Mechanisms of Natural Rubber Biosynthesis"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204701452160","@type":"Article","relationType":["isReferencedBy","isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Human .GAMMA..DELTA. 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