{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363388845672585216.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1105/tpc.104.029116"}},{"identifier":{"@type":"URI","@value":"http://academic.oup.com/plcell/article-pdf/17/4/1317/36879725/plcell_v17_4_1317.pdf"}}],"dc:title":[{"@value":"A Mitogen-Activated Protein Kinase Cascade Regulating Infection-Related Morphogenesis in <i>Magnaporthe grisea</i>"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title>\n               <jats:p>Many fungal pathogens invade plants by means of specialized infection structures called appressoria. In the rice (Oryza sativa) blast fungus Magnaporthe grisea, the pathogenicity mitogen-activated protein (MAP) kinase1 (PMK1) kinase is essential for appressorium formation and invasive growth. In this study, we functionally characterized the MST7 and MST11 genes of M. grisea that are homologous with the yeast MAP kinase kinase STE7 and MAP kinase kinase kinase STE11. Similar to the pmk1 mutant, the mst7 and mst11 deletion mutants were nonpathogenic and failed to form appressoria. When a dominant MST7 allele with S212D and T216E mutations was introduced into the mst7 or mst11 mutant, appressorium formation was restored in the resulting transformants. PMK1 phosphorylation also was detected in the vegetative hyphae and appressoria of transformants expressing the MST7  S212D T216E allele. However, appressoria formed by these transformants failed to penetrate and infect rice leaves, indicating that constitutively active MST7 only partially rescued the defects of the mst7 and mst11 mutants. The intracellular cAMP level was reduced in transformants expressing the MST7  S212D T216E allele. We also generated MST11 mutant alleles with the sterile alpha motif (SAM) and Ras-association (RA) domains deleted. Phenotype characterizations of the resulting transformants indicate that the SAM domain but not the RA domain is essential for the function of MST11. These data indicate that MST11, MST7, and PMK1 function as a MAP kinase cascade regulating infection-related morphogenesis in M. grisea. Although no direct interaction was detected between PMK1 and MST7 or MST11 in yeast two-hybrid assays, a homolog of yeast STE50 in M. grisea directly interacted with both MST7 and MST11 and may function as the adaptor protein for the MST11-MST7-PMK1 cascade.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1383388845672585217","@type":"Researcher","foaf:name":[{"@value":"Xinhua Zhao"}],"jpcoar:affiliationName":[{"@value":"Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907"}]},{"@id":"https://cir.nii.ac.jp/crid/1383388845672585218","@type":"Researcher","foaf:name":[{"@value":"Yangseon Kim"}],"jpcoar:affiliationName":[{"@value":"Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907"}]},{"@id":"https://cir.nii.ac.jp/crid/1383388845672585219","@type":"Researcher","foaf:name":[{"@value":"Gyungsoon Park"}],"jpcoar:affiliationName":[{"@value":"Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907"}]},{"@id":"https://cir.nii.ac.jp/crid/1383388845672585216","@type":"Researcher","foaf:name":[{"@value":"Jin-Rong Xu"}],"jpcoar:affiliationName":[{"@value":"Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"1532298X"}],"prism:publicationName":[{"@value":"The Plant Cell"}],"dc:publisher":[{"@value":"Oxford University Press (OUP)"}],"prism:publicationDate":"2005-04-01","prism:volume":"17","prism:number":"4","prism:startingPage":"1317","prism:endingPage":"1329"},"reviewed":"false","dc:rights":["https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model"],"url":[{"@id":"http://academic.oup.com/plcell/article-pdf/17/4/1317/36879725/plcell_v17_4_1317.pdf"}],"createdAt":"2005-03-05","modifiedAt":"2021-04-06","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360002219382107520","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The Aspergillus nidulans MAPK Module AnSte11-Ste50-Ste7-Fus3 Controls Development and Secondary Metabolism"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004237555747456","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Ras GTPase Activating Protein CoIra1 Is Involved in Infection-Related Morphogenesis by Regulating cAMP and MAPK Signaling Pathways through CoRas2 in Colletotrichum orbiculare"}]},{"@id":"https://cir.nii.ac.jp/crid/1360022307169390848","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Threonine synthase\n                  <i>CoTHR4</i>\n                  is involved in infection-related morphogenesis during the pre-penetration stage in\n                  <i>Colletotrichum orbiculare</i>"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285707362070016","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"MAPKK-encoding gene Ste7 in Bipolaris maydis is required for development and morphogenesis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360568467100942848","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"An adaptor protein BmSte50 interacts with BmSte11 MAPKKK and is involved in host infection, conidiation, melanization, and sexual development in Bipolaris maydis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360572092525938432","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"CoGRIM19 is required for invasive hyphal growth of Colletotrichum orbiculare inside epidermal cells of cucumber cotyledons"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282680185586176","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Cell signaling pathways of plant pathogenic fungi—roles in pathogenicity and fungicidal activity"},{"@language":"ja","@value":"植物病原糸状菌の細胞内シグナル伝達機構と殺菌剤および病原性"},{"@language":"ja-Kana","@value":"ショクブツ ビョウゲン シジョウキン ノ サイボウ ナイ シグナル デンタツ キコウ ト サッキンザイ オヨビ ビョウゲンセイ"},{"@value":"植物病原糸状菌の細胞内シグナル伝達機構と殺菌剤および病原性(新農薬創製への展望)"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1105/tpc.104.029116"},{"@type":"CROSSREF","@value":"10.1371/journal.pgen.1002816_references_DOI_N6GODctTcHByYluPkhjZ12LxGpW"},{"@type":"CROSSREF","@value":"10.1371/journal.pone.0109045_references_DOI_N6GODctTcHByYluPkhjZ12LxGpW"},{"@type":"CROSSREF","@value":"10.1101/543827_references_DOI_N6GODctTcHByYluPkhjZ12LxGpW"},{"@type":"CROSSREF","@value":"10.1016/j.myc.2019.10.003_references_DOI_N6GODctTcHByYluPkhjZ12LxGpW"},{"@type":"CROSSREF","@value":"10.1016/j.micpath.2021.104847_references_DOI_N6GODctTcHByYluPkhjZ12LxGpW"},{"@type":"CROSSREF","@value":"10.1584/jpestics.w12-27_references_DOI_N6GODctTcHByYluPkhjZ12LxGpW"},{"@type":"CROSSREF","@value":"10.1016/j.micpath.2019.103746_references_DOI_N6GODctTcHByYluPkhjZ12LxGpW"},{"@type":"CROSSREF","@value":"10.1016/j.myc.2014.05.003_references_DOI_N6GODctTcHByYluPkhjZ12LxGpW"}]}