Cellular dynamics associated with the genome-wide epigenetic reprogramming in migrating primordial germ cells in mice

DOI PDF PubMed 被引用文献68件 オープンアクセス
  • Yoshiyuki Seki
    Laboratory for Mammalian Germ Cell Biology, Center for Developmental Biology,RIKEN Kobe Institute, 2-2-3 Minatojima-Minamimachi, Chuo-ku, Kobe, 650-0047,Japan.
  • Masashi Yamaji
    Laboratory for Mammalian Germ Cell Biology, Center for Developmental Biology,RIKEN Kobe Institute, 2-2-3 Minatojima-Minamimachi, Chuo-ku, Kobe, 650-0047,Japan.
  • Yukihiro Yabuta
    Laboratory for Mammalian Germ Cell Biology, Center for Developmental Biology,RIKEN Kobe Institute, 2-2-3 Minatojima-Minamimachi, Chuo-ku, Kobe, 650-0047,Japan.
  • Mitsue Sano
    Laboratory for Mammalian Germ Cell Biology, Center for Developmental Biology,RIKEN Kobe Institute, 2-2-3 Minatojima-Minamimachi, Chuo-ku, Kobe, 650-0047,Japan.
  • Mayo Shigeta
    Laboratory for Mammalian Germ Cell Biology, Center for Developmental Biology,RIKEN Kobe Institute, 2-2-3 Minatojima-Minamimachi, Chuo-ku, Kobe, 650-0047,Japan.
  • Yasuhisa Matsui
    Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer, Tohoku University, 4-1 Seiryo-cho, Aoba-ku, Sendai 980-8575,Japan.
  • Yumiko Saga
    Department of Genetics and Division of Mammalian Development, National Institute of Genetics, SOKENDAI, 1111 Yata, Mishima, Shizuoka 411-8540,Japan.
  • Makoto Tachibana
    Department of Cell Biology, Institute for Virus Research, Kyoto University,Shogoin Kawara-cho, Kyoto 606-8507, Japan.
  • Yoichi Shinkai
    Department of Cell Biology, Institute for Virus Research, Kyoto University,Shogoin Kawara-cho, Kyoto 606-8507, Japan.
  • Mitinori Saitou
    Laboratory for Mammalian Germ Cell Biology, Center for Developmental Biology,RIKEN Kobe Institute, 2-2-3 Minatojima-Minamimachi, Chuo-ku, Kobe, 650-0047,Japan.

書誌事項

公開日
2007-07-15
DOI
  • 10.1242/dev.005611
公開者
The Company of Biologists

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説明

<jats:p>We previously reported that primordial germ cells (PGCs) in mice erase genome-wide DNA methylation and histone H3 lysine9 dimethylation (H3K9me2),and instead acquire high levels of tri-methylation of H3K27 (H3K27me3) during their migration, a process that might be crucial for the re-establishment of potential totipotency in the germline. We here explored a cellular dynamics associated with this epigenetic reprogramming. We found that PGCs undergo erasure of H3K9me2 and upregulation of H3K27me3 in a progressive, cell-by-cell manner, presumably depending on their developmental maturation. Before or concomitant with the onset of H3K9 demethylation, PGCs entered the G2 arrest of the cell cycle, which apparently persisted until they acquired high H3K27me3 levels. Interestingly, PGCs exhibited repression of RNA polymerase II-dependent transcription, which began after the onset of H3K9me2 reduction in the G2 phase and tapered off after the acquisition of high-level H3K27me3. The epigenetic reprogramming and transcriptional quiescence were independent from the function of Nanos3. We found that before H3K9 demethylation, PGCs exclusively repress an essential histone methyltransferase, GLP, without specifically upregulating histone demethylases. We suggest the possibility that active repression of an essential enzyme and subsequent unique cellular dynamics ensures successful implementation of genome-wide epigenetic reprogramming in migrating PGCs.</jats:p>

収録刊行物

  • Development

    Development 134 (14), 2627-2638, 2007-07-15

    The Company of Biologists

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