{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363388845894204416.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1046/j.1432-1033.2003.03740.x"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1432-1033.2003.03740.x"}},{"identifier":{"@type":"URI","@value":"https://febs.onlinelibrary.wiley.com/doi/pdf/10.1046/j.1432-1033.2003.03740.x"}},{"identifier":{"@type":"PMID","@value":"12899704"}}],"dc:title":[{"@value":"Identification of sodium salicylate as an <i>hsp</i> inducer using a simple screening system for stress response modulators in mammalian cells"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>As heat shock proteins (Hsps) are involved in protecting cells and also in the pathophysiology of diseases such as inflammation, cancer and neurodegenerative disorders, modulators of Hsp expression in mammalian cells would seem to be useful for the treatment of various diseases. In this study, we isolated mammalian cell lines for screening of Hsp modulators; mouse C3H10T1/2 cells stably transfected with a plasmid containing the mouse Hsp105 or human Hsp70B promoter upstream of a luciferase or β‐galactosidase reporter gene, respectively. Using these cells, we examined the effect of sodium salicylate (SA), which may induce the transcription of <jats:italic>hsp</jats:italic> genes, on stress response in mammalian cells. When these cells were treated with SA for 1 h at 37 °C, both promoter activities were up‐regulated by SA at concentrations of more than 45 m<jats:sc>m</jats:sc>. The activation of heat shock factor and the subsequent accumulation of Hsp105α and Hsp70 were detected in cells treated with SA at concentrations of more than 20 and 45 m<jats:sc>m</jats:sc>, respectively. Furthermore, SA induced resistance against a subsequent lethal stress. These findings suggested that SA is a potent <jats:italic>hsp</jats:italic> inducer, and may be used to protect cells against deleterious stressors.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1383388845894204416","@type":"Researcher","foaf:name":[{"@value":"Keiichi Ishihara"}]},{"@id":"https://cir.nii.ac.jp/crid/1383388845894204417","@type":"Researcher","foaf:name":[{"@value":"Kenji Horiguchi"}]},{"@id":"https://cir.nii.ac.jp/crid/1383388845894204419","@type":"Researcher","foaf:name":[{"@value":"Nobuyuki Yamagishi"}]},{"@id":"https://cir.nii.ac.jp/crid/1383388845894204418","@type":"Researcher","foaf:name":[{"@value":"Takumi Hatayama"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00142956"},{"@type":"EISSN","@value":"14321033"}],"prism:publicationName":[{"@value":"European Journal of Biochemistry"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2003-07-29","prism:volume":"270","prism:number":"16","prism:startingPage":"3461","prism:endingPage":"3468"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1432-1033.2003.03740.x"},{"@id":"https://febs.onlinelibrary.wiley.com/doi/pdf/10.1046/j.1432-1033.2003.03740.x"}],"createdAt":"2003-08-04","modifiedAt":"2023-10-16","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Mice","dc:title":"Mice"},{"@id":"https://cir.nii.ac.jp/all?q=Hot%20Temperature","dc:title":"Hot Temperature"},{"@id":"https://cir.nii.ac.jp/all?q=Sodium%20Salicylate","dc:title":"Sodium Salicylate"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Heat-Shock%20Proteins","dc:title":"Heat-Shock Proteins"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360848660427055872","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A role of Heat Shock Protein 70 in Photoreceptor Cell Death: Potential as a Novel Therapeutic Target in Retinal Degeneration"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679327667968","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Hyperthermia and Pharmacological Intervention of Heat Shock Proteins in Anticancer Treatments"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282680264206976","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Molecular Chaperone Inducers Facilitate the Functional Restoration of Temperature-sensitive Mutant p53 Protein"},{"@language":"ja","@value":"分子シャペロン誘導剤は温度感受性変異p53の機能回復を促進する"}]},{"@id":"https://cir.nii.ac.jp/crid/2051996266996504832","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Azaphilones produced by Penicillium maximae with their cell death-inducing activity on Adriamycin-treated cancer cell"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1046/j.1432-1033.2003.03740.x"},{"@type":"OPENAIRE","@value":"doi_dedup___::e4720d34207f8e752da9c5da6dd04064"},{"@type":"CROSSREF","@value":"10.1186/s41021-023-00261-w_references_DOI_HtYPXUmFMnysmx7C7j1iYObkWjs"},{"@type":"CROSSREF","@value":"10.3191/thermalmed.26.1_references_DOI_HtYPXUmFMnysmx7C7j1iYObkWjs"},{"@type":"CROSSREF","@value":"10.1111/cns.12471_references_DOI_HtYPXUmFMnysmx7C7j1iYObkWjs"},{"@type":"CROSSREF","@value":"10.3191/thermalmedicine.22.211_references_DOI_HtYPXUmFMnysmx7C7j1iYObkWjs"}]}