{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363388846184523904.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1242/jcs.01656"}},{"identifier":{"@type":"URI","@value":"http://journals.biologists.com/jcs/article-pdf/118/6/1267/1521406/1267.pdf"}},{"identifier":{"@type":"PMID","@value":"15741237"}},{"identifier":{"@type":"NAID","@value":"30002207252"}}],"dc:title":[{"@value":"Nectin-like molecule-1/TSLL1/SynCAM3: a neural tissue-specific immunoglobulin-like cell-cell adhesion molecule localizing at non-junctional contact sites of presynaptic nerve terminals, axons and glia cell processes"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>Nectins are Ca2+-independent immunoglobulin-like cell-cell adhesion molecules and comprise a family of four members. At the mossy fiber terminals of hippocampus, nectin-1 and nectin-3 localize at the presynaptic and postsynaptic sides of synaptic junctions, respectively, and their trans-interactions play a role in formation of synapses in cooperation with N-cadherin. Nectins are associated with the actin cytoskeleton through afadin, a nectin- and actin-filament-binding protein. Five nectin-like molecules (Necls) which have domain structures similar to those of nectins have been identified and here we characterize Necl-1/TSLL1/SynCAM3, from now on referred to as Necl-1. Tissue distribution analysis showed that Necl-1 was specifically expressed in the neural tissue. Immunofluorescence and immunoelectron microscopy revealed that Necl-1 localized at the contact sites among axons, their terminals, and glia cell processes that cooperatively formed synapses, axon bundles and myelinated axons. Necl-1 showed Ca2+-independent homophilic cell-cell adhesion activity. It furthermore showed Ca2+-independent heterophilic cell-cell adhesion activity with Necl-2/IGSF4/RA175/SgIGSF/TSLC1/SynCAM1 from now on referred to as Necl-2, nectin-1 and nectin-3, but not with Necl-5 or nectin-2. The C-terminal cytoplasmic region of Necl-1 did not bind afadin but bound membrane-associated guanylate kinase subfamily members that contain the L27 domain, including Dlg3, Pals2 and CASK. These results indicate that Necl-1 is a neural-tissue-specific Ca2+-independent immunoglobulin-like cell-cell adhesion molecule which potentially has membrane-associated guanylate kinase subfamily member-binding activity and localizes at the non-junctional cell-cell contact sites.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1383388846184523909","@type":"Researcher","foaf:name":[{"@value":"Shigeki Kakunaga"}],"jpcoar:affiliationName":[{"@value":"Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine/Faculty of Medicine, Suita 565-0871, Osaka, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1383388846184523910","@type":"Researcher","foaf:name":[{"@value":"Wataru Ikeda"}],"jpcoar:affiliationName":[{"@value":"Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine/Faculty of Medicine, Suita 565-0871, Osaka, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1383388846184523908","@type":"Researcher","foaf:name":[{"@value":"Shinsuke Itoh"}],"jpcoar:affiliationName":[{"@value":"Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine/Faculty of Medicine, Suita 565-0871, Osaka, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1383388846184523907","@type":"Researcher","foaf:name":[{"@value":"Maki Deguchi-Tawarada"}],"jpcoar:affiliationName":[{"@value":"KAN Research Institute Incorporated, 93 Chudoji-Awatamachi, Shimogyo-ku, Kyoto 600-8815, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1420282801186197120","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"40401806"},{"@type":"NRID","@value":"1000040401806"},{"@type":"NRID","@value":"9000003632127"},{"@type":"NRID","@value":"9000391753316"},{"@type":"NRID","@value":"9000347064356"},{"@type":"NRID","@value":"9000259342144"},{"@type":"NRID","@value":"9000019140132"},{"@type":"NRID","@value":"9000309161373"},{"@type":"NRID","@value":"9000331423945"},{"@type":"NRID","@value":"9000391753423"},{"@type":"NRID","@value":"9000345193356"},{"@type":"NRID","@value":"9000001837554"},{"@type":"NRID","@value":"9000399589300"},{"@type":"NRID","@value":"9000009950364"},{"@type":"NRID","@value":"9000018516319"},{"@type":"NRID","@value":"9000003791305"},{"@type":"NRID","@value":"9000345193423"},{"@type":"NRID","@value":"9000402924439"},{"@type":"NRID","@value":"9000018562078"},{"@type":"NRID","@value":"9000331424016"},{"@type":"NRID","@value":"9000309161497"},{"@type":"NRID","@value":"9000283718661"},{"@type":"NRID","@value":"9000398819169"},{"@type":"NRID","@value":"9000019951997"},{"@type":"NRID","@value":"9000410014879"},{"@type":"NRID","@value":"9000021539695"},{"@type":"NRID","@value":"9000238098416"},{"@type":"NRID","@value":"9000309162735"},{"@type":"NRID","@value":"9000402924337"},{"@type":"NRID","@value":"9000380122654"},{"@type":"NRID","@value":"9000333924092"},{"@type":"NRID","@value":"9000309160802"},{"@type":"NRID","@value":"9000264272296"},{"@type":"NRID","@value":"9000375852227"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/ohtsuka"}],"foaf:name":[{"@value":"Toshihisa Ohtsuka"}],"jpcoar:affiliationName":[{"@value":"KAN Research Institute Incorporated, 93 Chudoji-Awatamachi, Shimogyo-ku, Kyoto 600-8815, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1383388846184523905","@type":"Researcher","foaf:name":[{"@value":"Akira Mizoguchi"}],"jpcoar:affiliationName":[{"@value":"Department of Anatomy, School of Medicine, Mie University, Tsu 514-8507, Mie, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1383388846184523906","@type":"Researcher","foaf:name":[{"@value":"Yoshimi Takai"}],"jpcoar:affiliationName":[{"@value":"Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine/Faculty of Medicine, Suita 565-0871, Osaka, Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"14779137"},{"@type":"PISSN","@value":"00219533"}],"prism:publicationName":[{"@value":"Journal of Cell Science"}],"dc:publisher":[{"@value":"The Company of Biologists"}],"prism:publicationDate":"2005-03-15","prism:volume":"118","prism:number":"6","prism:startingPage":"1267","prism:endingPage":"1277"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"http://journals.biologists.com/jcs/article-pdf/118/6/1267/1521406/1267.pdf"}],"createdAt":"2005-03-02","modifiedAt":"2021-04-25","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Cytoplasm","dc:title":"Cytoplasm"},{"@id":"https://cir.nii.ac.jp/all?q=Time%20Factors","dc:title":"Time Factors"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Communication","dc:title":"Cell Communication"},{"@id":"https://cir.nii.ac.jp/all?q=Hippocampus","dc:title":"Hippocampus"},{"@id":"https://cir.nii.ac.jp/all?q=Mice","dc:title":"Mice"},{"@id":"https://cir.nii.ac.jp/all?q=Cerebellum","dc:title":"Cerebellum"},{"@id":"https://cir.nii.ac.jp/all?q=Tissue%20Distribution","dc:title":"Tissue 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