{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363670318509199232.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1191/096120301701548508"}},{"identifier":{"@type":"URI","@value":"https://journals.sagepub.com/doi/pdf/10.1191/096120301701548508"}}],"dc:title":[{"@value":"Dual binding capabilities of anti-double-stranded DNA antibodies and                 anti-ribosomal phosphoprotein (P) antibodies"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p> The aimof this study is to identify distinctive properties of pathogenic anti-double stranded DNA antibodies and anti-ribosomal P antibodies. </jats:p><jats:p> The binding activity of anti-dsDNA and anti-ribosomal P antibodies to their cognate antigens in 0.15 M and 1.5 M NaCl solutions on ELISA was examined. All anti-dsDNA and anti-ribosomal P antibodies exhibited a loss of their binding activity from37.5 to 100% and from2.3 to 97.4% in high ionic strength buffers, respectively. In contrast, anti-U1RNP antibodies and anti-Ro/SSA antibodies lost from0 to 32.7% and from0 to 40.1% of their binding activity, respectively. Anti-dsDNA and anti-ribosomal P antibodies from patients with nephropathy showed significantly higher binding activity in high ionic strength buffers than those frompatients without nephropathy. Study of paired sera fromlupus nephritis patients revealed that anti-dsDNA and anti-ribosomal P antibodies frompatients during disease flare show stronger binding activity in high ionic strength buffer than those during remission. Most anti-dsDNA and anti-ribosomal P antibodies bind their antigens by ionic interactions that are sensitive to high salt. Such dual binding capability of anti-dsDNA and anti-ribosomal P antibodies may underlie their multiple cross reactivities to various epitopes and help elucidate the pathogenic potential of autoantibody subsets. </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380848656230091266","@type":"Researcher","foaf:name":[{"@value":"I Takeda"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318509199235","@type":"Researcher","foaf:name":[{"@value":"K Rayno"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318509199232","@type":"Researcher","foaf:name":[{"@value":"F B Movafagh"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318509199236","@type":"Researcher","foaf:name":[{"@value":"M Wolfson-Reichlin"}],"jpcoar:affiliationName":[{"@value":"Arthritis and Immunology Program, Oklahoma Medical Research Foundation,                         Oklahoma University Health Sciences Center, Oklahoma City, Oklahoma, USA"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318509199234","@type":"Researcher","foaf:name":[{"@value":"M Reichlin"}],"jpcoar:affiliationName":[{"@value":"Arthritis and Immunology Program, Oklahoma Medical Research Foundation,                         Oklahoma University Health Sciences Center, Oklahoma City, Oklahoma, USA;                         Rheumatology, Immunology and Allergy Section, Department of Medicine,                         Oklahoma University Health Sciences Center, Oklahoma City, Oklahoma, USA;                         825 Northeast 13th Street, Oklahoma City, OK 73104, USA"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"09612033"},{"@type":"EISSN","@value":"14770962"}],"prism:publicationName":[{"@value":"Lupus"}],"dc:publisher":[{"@value":"SAGE Publications"}],"prism:publicationDate":"2001-12","prism:volume":"10","prism:number":"12","prism:startingPage":"857","prism:endingPage":"865"},"reviewed":"false","dc:rights":["https://journals.sagepub.com/page/policies/text-and-data-mining-license"],"url":[{"@id":"https://journals.sagepub.com/doi/pdf/10.1191/096120301701548508"}],"createdAt":"2003-11-12","modifiedAt":"2025-02-28","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1390282681283219072","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"ANTI-RIBOSOMAL P ANTIBODIES ARE ASSOCIATED WITH NEPHRITIS, VASCULAR THROMBOSIS AND LYMPHOCYTOPENIA IN PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS"}]},{"@id":"https://cir.nii.ac.jp/crid/2051151842050499840","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Anti-ribosomal P antibodies and lupus nephritis"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1191/096120301701548508"},{"@type":"CROSSREF","@value":"10.5387/fms.51.11_references_DOI_BtDvvtx2A7iAo401x0CNycX5Uoc"},{"@type":"CROSSREF","@value":"10.1007/s10157-011-0462-9_references_DOI_BtDvvtx2A7iAo401x0CNycX5Uoc"}]}