{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363670318568771584.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1111/tpj.12664"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Ftpj.12664"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/tpj.12664"}}],"dc:title":[{"@value":"An <i>O‐</i>methyltransferase modifies accumulation of methylated anthocyanins in seedlings of tomato"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Summary</jats:title><jats:p>Anthocyanins contribute to the appearance of fruit by conferring to them a red, blue or purple colour. In a food context, they have also been suggested to promote consumer health. In purple tomato tissues, such as hypocotyls, stems and purple fruits, various anthocyanins accumulate. These molecules have characteristic patterns of modification, including hydroxylations, methylations, glycosylations and acylations. The genetic basis for many of these modifications has not been fully elucidated, and nor has their role in the functioning of anthocyanins. In this paper, Anth<jats:styled-content style=\"fixed-case\">OMT</jats:styled-content>, an <jats:italic>O‐</jats:italic>methyltransferase (<jats:styled-content style=\"fixed-case\">OMT</jats:styled-content>) mediating the methylation of anthocyanins, has been identified and functionally characterized using a combined metabolomics and transcriptomics approach. Gene candidates were selected from the draft tomato genome, and their expression was subsequently monitored in a tomato seedling system comprising three tissues and involving several time points. In addition, we also followed gene expression in wild‐type red and purple transgenic tomato fruits expressing <jats:italic>Rosea1</jats:italic> and <jats:italic>Delila</jats:italic> transcription factors. Of the 57 candidates identified, only a single <jats:styled-content style=\"fixed-case\">OMT</jats:styled-content> gene showed patterns strongly correlating with both accumulation of anthocyanins and expression of anthocyanin biosynthesis genes. This candidate (<jats:italic>Anth<jats:styled-content style=\"fixed-case\">OMT</jats:styled-content></jats:italic>) was compared to a closely related caffeoyl CoA <jats:styled-content style=\"fixed-case\">OMT</jats:styled-content> by recombinant expression in <jats:italic>Escherichia coli</jats:italic>, and then tested for substrate specificity. Anth<jats:styled-content style=\"fixed-case\">OMT</jats:styled-content> showed a strong affinity for glycosylated anthocyanins, while other flavonoid glycosides and aglycones were much less preferred. Gene silencing experiments with <jats:italic>Anth<jats:styled-content style=\"fixed-case\">OMT</jats:styled-content></jats:italic> resulted in reduced levels of the predominant methylated anthocyanins. This confirms the role of this enzyme in the diversification of tomato anthocyanins.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1383670318568771587","@type":"Researcher","foaf:name":[{"@value":"Maria Victoria Gomez Roldan"}],"jpcoar:affiliationName":[{"@value":"BU Biosciences Plant Research International Wageningen University and Research Centre PO Box 16 6700 AA Wageningen The Netherlands"},{"@value":"Netherlands Consortium for Systems Biology PO Box 94215 1090 GE Amsterdam The Netherlands"},{"@value":"Netherlands Metabolomics Centre Einsteinweg 55 2333 CC Leiden The Netherlands"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318568771585","@type":"Researcher","foaf:name":[{"@value":"Nikolay Outchkourov"}],"jpcoar:affiliationName":[{"@value":"BU Biosciences Plant Research International Wageningen University and Research Centre PO Box 16 6700 AA Wageningen The Netherlands"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318568771592","@type":"Researcher","foaf:name":[{"@value":"Adèle van Houwelingen"}],"jpcoar:affiliationName":[{"@value":"BU Biosciences Plant Research International Wageningen University and Research Centre PO Box 16 6700 AA Wageningen The Netherlands"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318568771591","@type":"Researcher","foaf:name":[{"@value":"Michiel Lammers"}],"jpcoar:affiliationName":[{"@value":"BU Biosciences Plant Research International Wageningen University and Research Centre PO Box 16 6700 AA Wageningen The Netherlands"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318568771590","@type":"Researcher","foaf:name":[{"@value":"Irene Romero de la Fuente"}],"jpcoar:affiliationName":[{"@value":"BU Biosciences Plant Research International Wageningen University and Research Centre PO Box 16 6700 AA Wageningen The Netherlands"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318568771588","@type":"Researcher","foaf:name":[{"@value":"Noa Ziklo"}],"jpcoar:affiliationName":[{"@value":"Department of Plant Sciences Weizmann Institute of Science Rehovot 76100 Israel"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318568771586","@type":"Researcher","foaf:name":[{"@value":"Asaph Aharoni"}],"jpcoar:affiliationName":[{"@value":"Department of Plant Sciences Weizmann Institute of Science Rehovot 76100 Israel"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318568771589","@type":"Researcher","foaf:name":[{"@value":"Robert D. Hall"}],"jpcoar:affiliationName":[{"@value":"BU Biosciences Plant Research International Wageningen University and Research Centre PO Box 16 6700 AA Wageningen The Netherlands"},{"@value":"Netherlands Consortium for Systems Biology PO Box 94215 1090 GE Amsterdam The Netherlands"},{"@value":"Netherlands Metabolomics Centre Einsteinweg 55 2333 CC Leiden The Netherlands"},{"@value":"Laboratory of Plant Physiology Wageningen University PO Box 658 6700 AR Wageningen The Netherlands"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318568771584","@type":"Researcher","foaf:name":[{"@value":"Jules Beekwilder"}],"jpcoar:affiliationName":[{"@value":"BU Biosciences Plant Research International Wageningen University and Research Centre PO Box 16 6700 AA Wageningen The Netherlands"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"09607412"},{"@type":"EISSN","@value":"1365313X"}],"prism:publicationName":[{"@value":"The Plant Journal"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2014-10-15","prism:volume":"80","prism:number":"4","prism:startingPage":"695","prism:endingPage":"708"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Ftpj.12664"},{"@id":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/tpj.12664"}],"createdAt":"2014-09-16","modifiedAt":"2023-10-01","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360009142688302080","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Expression and functional analysis of the nobiletin biosynthesis-related gene CitOMT in citrus fruit"}]},{"@id":"https://cir.nii.ac.jp/crid/1360016861577603200","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Diversification of Chemical Structures of Methoxylated Flavonoids and Genes Encoding Flavonoid-O-Methyltransferases"}]},{"@id":"https://cir.nii.ac.jp/crid/1360286992114819712","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Exploiting Natural Variation in Tomato to Define Pathway Structure and Metabolic Regulation of Fruit Polyphenolics in the Lycopersicum Complex"}]},{"@id":"https://cir.nii.ac.jp/crid/1360572092526451072","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Identification of a Unique Type of Isoflavone <i>O</i>-Methyltransferase, GmIOMT1, Based on Multi-Omics Analysis of Soybean under Biotic Stress"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204327497728","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Flower color modification in <i>Rosa hybrida</i> by expressing the <i>S</i>-adenosylmethionine: anthocyanin 3′,5′-<i>O</i>-methyltransferase gene from <i>Torenia hybrida</i>"},{"@value":"Flower color modification in Rosa hybrida by expressing the S-adenosylmethionine : anthocyanin 3',5'-O-methyltransferase gene from Torenia hybrida"},{"@value":"Flower color modification in Rosa hybrida by expressing the S-adenosylmethionine : anthocyanin 3′,5′-O-methyltransferase gene from Torenia hybrida"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204329165440","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Molecular cloning of flavonoid biosynthetic genes and biochemical characterization of anthocyanin <i>O</i>-methyltransferase of <i>Nemophila menziesii</i> Hook. and Arn."},{"@value":"Molecular cloning of flavonoid biosynthetic genes and biochemical characterization of anthocyanin O-methyltransferase of Nemophila menziesii Hook. and Arn"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1111/tpj.12664"},{"@type":"CROSSREF","@value":"10.1038/s41598-020-72277-z_references_DOI_aG1Q0lXFw7x8ouSuABfQ4Tf077k"},{"@type":"CROSSREF","@value":"10.3390/plants11040564_references_DOI_aG1Q0lXFw7x8ouSuABfQ4Tf077k"},{"@type":"CROSSREF","@value":"10.1016/j.molp.2020.04.004_references_DOI_aG1Q0lXFw7x8ouSuABfQ4Tf077k"},{"@type":"CROSSREF","@value":"10.5511/plantbiotechnology.18.0104a_references_DOI_aG1Q0lXFw7x8ouSuABfQ4Tf077k"},{"@type":"CROSSREF","@value":"10.1093/pcp/pcaa112_references_DOI_aG1Q0lXFw7x8ouSuABfQ4Tf077k"},{"@type":"CROSSREF","@value":"10.5511/plantbiotechnology.15.0205a_references_DOI_aG1Q0lXFw7x8ouSuABfQ4Tf077k"}]}