Stemar‐13‐ene synthase, a diterpene cyclase involved in the biosynthesis of the phytoalexin oryzalexin S in rice
書誌事項
- 公開日
- 2004-07-14
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1016/j.febslet.2004.07.002
- 公開者
- Wiley
この論文をさがす
説明
<jats:p>In suspension‐cultured rice cells, diterpenoid phytoalexins are produced in response to exogenously applied elicitors. We isolated a cDNA encoding a diterpene cyclase, OsDTC2, from suspension‐cultured rice cells treated with a chitin elicitor. The <jats:italic>OsDTC2</jats:italic> cDNA was overexpressed in <jats:italic>Escherichia coli</jats:italic> as a fusion protein with glutathione <jats:italic>S</jats:italic>‐transferase, and the recombinant OsDTC2 was indicated to function as stemar‐13‐ene synthase that converted <jats:italic>syn</jats:italic>‐copalyl diphosphate to stemar‐13‐ene, a putative diterpene hydrocarbon precursor of the phytoalexin oryzalexin S. The level of <jats:italic>OsDTC2</jats:italic> mRNA in suspension‐cultured rice cells began to increase 3 h after addition of the elicitor and reached the maximum after 8 h. The expression of <jats:italic>OsDTC2</jats:italic> was also induced in UV‐irradiated rice leaves. In addition, we indicated that stemar‐13‐ene accumulated in the chitin‐elicited suspension‐cultured rice cells and the UV‐irradiated rice leaves.</jats:p>
収録刊行物
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- FEBS Letters
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FEBS Letters 571 (1-3), 182-186, 2004-07-14
Wiley
