{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363670318901198592.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/j.febslet.2004.07.002"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2Fj.febslet.2004.07.002"}},{"identifier":{"@type":"URI","@value":"https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/j.febslet.2004.07.002"}},{"identifier":{"@type":"PMID","@value":"15280039"}},{"identifier":{"@type":"NAID","@value":"80016776664"}}],"dc:title":[{"@value":"Stemar‐13‐ene synthase, a diterpene cyclase involved in the biosynthesis of the phytoalexin oryzalexin S in rice"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>In suspension‐cultured rice cells, diterpenoid phytoalexins are produced in response to exogenously applied elicitors. We isolated a cDNA encoding a diterpene cyclase, OsDTC2, from suspension‐cultured rice cells treated with a chitin elicitor. The <jats:italic>OsDTC2</jats:italic> cDNA was overexpressed in <jats:italic>Escherichia coli</jats:italic> as a fusion protein with glutathione <jats:italic>S</jats:italic>‐transferase, and the recombinant OsDTC2 was indicated to function as stemar‐13‐ene synthase that converted <jats:italic>syn</jats:italic>‐copalyl diphosphate to stemar‐13‐ene, a putative diterpene hydrocarbon precursor of the phytoalexin oryzalexin S. The level of <jats:italic>OsDTC2</jats:italic> mRNA in suspension‐cultured rice cells began to increase 3 h after addition of the elicitor and reached the maximum after 8 h. The expression of <jats:italic>OsDTC2</jats:italic> was also induced in UV‐irradiated rice leaves. In addition, we indicated that stemar‐13‐ene accumulated in the chitin‐elicited suspension‐cultured rice cells and the UV‐irradiated rice leaves.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1383670318901198598","@type":"Researcher","foaf:name":[{"@value":"Tadahiro Nemoto"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318901198722","@type":"Researcher","foaf:name":[{"@value":"Eun-Min Cho"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318901198720","@type":"Researcher","foaf:name":[{"@value":"Atsushi Okada"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318901198726","@type":"Researcher","foaf:name":[{"@value":"Kazunori Okada"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318901198725","@type":"Researcher","foaf:name":[{"@value":"Kazuko Otomo"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318901198721","@type":"Researcher","foaf:name":[{"@value":"Yuri Kanno"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318901198723","@type":"Researcher","foaf:name":[{"@value":"Tomonobu Toyomasu"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318901198592","@type":"Researcher","foaf:name":[{"@value":"Wataru Mitsuhashi"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318901198724","@type":"Researcher","foaf:name":[{"@value":"Takeshi Sassa"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318901198596","@type":"Researcher","foaf:name":[{"@value":"Eiichi 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Shibuya"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318901198595","@type":"Researcher","foaf:name":[{"@value":"Makoto Nishiyama"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318901198593","@type":"Researcher","foaf:name":[{"@value":"Hideaki Nojiri"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318901198594","@type":"Researcher","foaf:name":[{"@value":"Hisakazu Yamane"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00145793"},{"@type":"EISSN","@value":"18733468"}],"prism:publicationName":[{"@value":"FEBS Letters"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2004-07-14","prism:volume":"571","prism:number":"1-3","prism:startingPage":"182","prism:endingPage":"186"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2Fj.febslet.2004.07.002"},{"@id":"https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/j.febslet.2004.07.002"}],"createdAt":"2004-07-16","modifiedAt":"2023-10-12","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Base%20Sequence","dc:title":"Base Sequence"},{"@id":"https://cir.nii.ac.jp/all?q=Diterpene%20cyclase","dc:title":"Diterpene cyclase"},{"@id":"https://cir.nii.ac.jp/all?q=Oryza","dc:title":"Oryza"},{"@id":"https://cir.nii.ac.jp/all?q=Oryzalexin%20S","dc:title":"Oryzalexin S"},{"@id":"https://cir.nii.ac.jp/all?q=Kinetics","dc:title":"Kinetics"},{"@id":"https://cir.nii.ac.jp/all?q=Phytoalexin","dc:title":"Phytoalexin"},{"@id":"https://cir.nii.ac.jp/all?q=Oryza%20sativa%20L.","dc:title":"Oryza sativa L."},{"@id":"https://cir.nii.ac.jp/all?q=RNA,%20Messenger","dc:title":"RNA, Messenger"},{"@id":"https://cir.nii.ac.jp/all?q=Carbon-Carbon%20Lyases","dc:title":"Carbon-Carbon Lyases"},{"@id":"https://cir.nii.ac.jp/all?q=Diterpenes","dc:title":"Diterpenes"},{"@id":"https://cir.nii.ac.jp/all?q=Plant%20Lectins","dc:title":"Plant Lectins"},{"@id":"https://cir.nii.ac.jp/all?q=Stemar-13-ene","dc:title":"Stemar-13-ene"},{"@id":"https://cir.nii.ac.jp/all?q=Cells,%20Cultured","dc:title":"Cells, Cultured"},{"@id":"https://cir.nii.ac.jp/all?q=DNA%20Primers","dc:title":"DNA Primers"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360285709646642304","@type":"Article","resourceType":"学術雑誌論文(journal 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