{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363670318919960704.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1038/s41467-018-04440-0"}},{"identifier":{"@type":"URI","@value":"https://www.nature.com/articles/s41467-018-04440-0.pdf"}},{"identifier":{"@type":"URI","@value":"https://www.nature.com/articles/s41467-018-04440-0"}},{"identifier":{"@type":"DOI","@value":"10.5451/unibas-ep70534"}},{"identifier":{"@type":"DOI","@value":"10.3929/ethz-b-000265945"}},{"identifier":{"@type":"PMID","@value":"29769518"}},{"identifier":{"@type":"HANDLE","@value":"20.500.11850/265945"}}],"dc:title":[{"@value":"A cell-penetrating artificial metalloenzyme regulates a gene switch in a designer mammalian cell"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title>\n                  <jats:p>Complementing enzymes in their native environment with either homogeneous or heterogeneous catalysts is challenging due to the sea of functionalities present within a cell. To supplement these efforts, artificial metalloenzymes are drawing attention as they combine attractive features of both homogeneous catalysts and enzymes. Herein we show that such hybrid catalysts consisting of a metal cofactor, a cell-penetrating module, and a protein scaffold are taken up into HEK-293T cells where they catalyze the uncaging of a hormone. This bioorthogonal reaction causes the upregulation of a gene circuit, which in turn leads to the expression of a nanoluc-luciferase. Relying on the biotin–streptavidin technology, variation of the biotinylated ruthenium complex: the biotinylated cell-penetrating poly(disulfide) ratio can be combined with point mutations on streptavidin to optimize the catalytic uncaging of an allyl-carbamate-protected thyroid hormone triiodothyronine. These results demonstrate that artificial metalloenzymes offer highly modular tools to perform bioorthogonal catalysis in live HEK cells.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1383670318919960710","@type":"Researcher","foaf:name":[{"@value":"Yasunori Okamoto"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318919960704","@type":"Researcher","foaf:name":[{"@value":"Ryosuke Kojima"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318919960711","@type":"Researcher","foaf:name":[{"@value":"Fabian Schwizer"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318919960707","@type":"Researcher","foaf:name":[{"@value":"Eline Bartolami"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318919960708","@type":"Researcher","foaf:name":[{"@value":"Tillmann Heinisch"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318919960709","@type":"Researcher","foaf:name":[{"@value":"Stefan Matile"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318919960706","@type":"Researcher","foaf:name":[{"@value":"Martin Fussenegger"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670318919960705","@type":"Researcher","foaf:name":[{"@value":"Thomas R. Ward"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"20411723"}],"prism:publicationName":[{"@value":"Nature Communications"}],"dc:publisher":[{"@value":"Springer Science and Business Media LLC"}],"prism:publicationDate":"2018-05-16","prism:volume":"9","prism:number":"1","prism:startingPage":"1943"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["https://creativecommons.org/licenses/by/4.0","https://creativecommons.org/licenses/by/4.0"],"url":[{"@id":"https://www.nature.com/articles/s41467-018-04440-0.pdf"},{"@id":"https://www.nature.com/articles/s41467-018-04440-0"}],"createdAt":"2018-05-10","modifiedAt":"2022-12-20","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=info:eu-repo/classification/ddc/540","dc:title":"info:eu-repo/classification/ddc/540"},{"@id":"https://cir.nii.ac.jp/all?q=Molecular%20Structure","dc:title":"Molecular Structure"},{"@id":"https://cir.nii.ac.jp/all?q=Science","dc:title":"Science"},{"@id":"https://cir.nii.ac.jp/all?q=Q","dc:title":"Q"},{"@id":"https://cir.nii.ac.jp/all?q=Biotin","dc:title":"Biotin"},{"@id":"https://cir.nii.ac.jp/all?q=Metalloendopeptidases","dc:title":"Metalloendopeptidases"},{"@id":"https://cir.nii.ac.jp/all?q=Stereoisomerism","dc:title":"Stereoisomerism"},{"@id":"https://cir.nii.ac.jp/all?q=Article","dc:title":"Article"},{"@id":"https://cir.nii.ac.jp/all?q=Catalysis","dc:title":"Catalysis"},{"@id":"https://cir.nii.ac.jp/all?q=Ruthenium","dc:title":"Ruthenium"},{"@id":"https://cir.nii.ac.jp/all?q=HEK293%20Cells","dc:title":"HEK293 Cells"},{"@id":"https://cir.nii.ac.jp/all?q=Humans","dc:title":"Humans"},{"@id":"https://cir.nii.ac.jp/all?q=Point%20Mutation","dc:title":"Point Mutation"},{"@id":"https://cir.nii.ac.jp/all?q=Triiodothyronine","dc:title":"Triiodothyronine"},{"@id":"https://cir.nii.ac.jp/all?q=Biotinylation","dc:title":"Biotinylation"},{"@id":"https://cir.nii.ac.jp/all?q=Streptavidin","dc:title":"Streptavidin"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050863782914881792","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Artificial metalloenzymes based on protein assembly"}]},{"@id":"https://cir.nii.ac.jp/crid/1360013168831790848","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Intracellular Unnatural Catalysis Enabled by an Artificial Metalloenzyme"}]},{"@id":"https://cir.nii.ac.jp/crid/1360017282442230272","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Catalysis medicine: Participating in the 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