Validation of human periodontal ligament‐derived cells as a reliable source for cytotherapeutic use

DOI Web Site Web Site PubMed 35 Citations

Search this article

Description

<jats:sec><jats:label /><jats:p>Iwata T, Yamato M, Zhang Z, Mukobata S, Washio K, Ando T, Feijen J, Okano T, Ishikawa I. Validation of human periodontal ligament‐derived cells as a reliable source for cytotherapeutic use. J Clin Periodontol 2010; doi: 10.1111/j.1600‐051X.2010.01597.x.</jats:p></jats:sec><jats:sec><jats:title>Abstract</jats:title><jats:p><jats:bold>Aim: </jats:bold> Periodontal ligament (PDL) is a reliable cell source for periodontal regeneration. In this study, an optimal protocol for the extraction, expansion, and characterization of human PDL (hPDL) cells was examined for clinical trials.</jats:p><jats:p><jats:bold>Materials and Methods: </jats:bold> hPDL tissues were obtained from 41 surgically extracted teeth and digested with enzymes. Human adipose‐derived stem cells (hADSCs), bone marrow‐derived mesenchymal stem cells (hBMMSCs), and gingival fibroblasts (hGFs) were used for comparison. For each sample, the proliferative capacity, colony‐forming ability, alkaline phosphatase activity, differentiation ability, the cell surface antigens, gene expression, and regenerative potential were examined.</jats:p><jats:p><jats:bold>Results: </jats:bold> hPDL cells were more successfully extracted with collagenase/dispase [29/30 (96.7%)] than with trypsin/EDTA [8/11 (72.7%)], and exhibited osteogenic potential both in vitro and in vivo. The proliferation of hPDL cells was rapid at a low cell density. hPDL cells frequently differentiated into cementoblastic/osteoblastic lineage (∼60%). In contrast, their adipogenic and chondrogenic potentials were lower than those of hADSCs and hBMMSCs. Some genes (NCAM1, S100A4, and periostin) were preferentially expressed in hPDL cells compared with those of hBMMSCs and hGFs. Immunohistochemical studies revealed the expressions of S100A4 and periostin in hPDL tissue.</jats:p><jats:p><jats:bold>Conclusion: </jats:bold> A protocol for the successful cultivation and validation of hPDL cells is proposed for clinical settings.</jats:p></jats:sec>

Journal

Citations (35)*help

See more

Keywords

Report a problem

Back to top