{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363670319222742016.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1083/jcb.98.4.1523"}},{"identifier":{"@type":"URI","@value":"https://rupress.org/jcb/article-pdf/98/4/1523/1642436/1523.pdf"}},{"identifier":{"@type":"NAID","@value":"30017404816"}}],"dc:title":[{"@value":"Organization of mammalian neurofilament polypeptides within the neuronal cytoskeleton."}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>Neurofilaments in the axons of mammalian spinal cord neurons are extensively cross-linked; consequently, the filaments and their cross-bridges compose a three-dimensional lattice. We have used antibody decoration in situ combined with tissue preparation by the quick-freeze, deep-etch technique to locate three neurofilament polypeptides (195, 145, and 73 Kd) within this lattice. When antibodies against each polypeptide were incubated with detergent-extracted, formaldehyde-fixed samples of rabbit spinal cord, each antibody assumed a characteristic distribution: anti-73-Kd decorated the neurofilament core uniformly, but not the cross-bridges; anti-145-Kd also decorated the core, but less uniformly; sometimes the anti-145-Kd antibodies were located over the bases of cross-bridges. In contrast, anti-195-Kd primarily decorated the cross-bridges between the neurofilaments. These observations show that the 73-Kd polypeptide is a component of the central core of neurofilaments, and that the 195-Kd polypeptide is a component of the inter-neurofilamentous cross-bridges. It is consistent with this conclusion that we found few cross-bridges between neurofilaments in the optic nerves of neonatal rabbits during a developmental period when the ratio of 195 to 73 or 145-Kd polypeptides is much lower than in adults. The ratio of 195-Kd polypeptide to the other two neurofilament polypeptides also appeared much lower in the cell bodies and dendrites than in axons of adult spinal cord neurons, when the dispositions of the three polypeptides were studied by immunofluorescence experiments. The cell bodies apparently contain neurofilaments composed primarily of 145- and 73-Kd polypeptides, because we observed antibody decoration of individual neurofilaments in the cell bodies with anti-73- and -145-Kd, but not with anti-195-Kd. We conclude that the 195-Kd polypeptide participates in a cross-linking function, and that this function is, at least in certain neurons, most prevalent in the mature axon.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1580854174161006208","@type":"Researcher","foaf:name":[{"@value":"N Hirokawa"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670319222741889","@type":"Researcher","foaf:name":[{"@value":"M A Glicksman"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670319222741888","@type":"Researcher","foaf:name":[{"@value":"M B Willard"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00219525"},{"@type":"EISSN","@value":"15408140"},{"@type":"PISSN","@value":"https://id.crossref.org/issn/00219525"}],"prism:publicationName":[{"@value":"The Journal of cell biology"}],"dc:publisher":[{"@value":"Rockefeller University Press"}],"prism:publicationDate":"1984-04-01","prism:volume":"98","prism:number":"4","prism:startingPage":"1523","prism:endingPage":"1536"},"reviewed":"false","url":[{"@id":"https://rupress.org/jcb/article-pdf/98/4/1523/1642436/1523.pdf"}],"createdAt":"2004-05-14","modifiedAt":"2023-07-24","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360565165796587776","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Inhibition of nestin suppresses stem cell phenotype of glioblastomas through the alteration of post-translational modification of heat shock protein HSPA8/HSC71"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567182511535744","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Role of phosphorylation on the structural dynamics and function of types III and IV intermediate filaments"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567184585347584","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"From electron microscopy to molecular cell biology, molecular genetics and structural biology: intracellular transport and kinesin superfamily proteins, KIFs: genes, structure, dynamics and functions"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567184855788032","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Systemic Administration of Small Interfering RNA Targeting Human Nestin Inhibits Pancreatic Cancer Cell Proliferation and Metastasis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848655352323072","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Neurofilament Cross-Bridge – A Structure Associated Specifically with the Neurofilament Among the Intermediate Filament Family"}]},{"@id":"https://cir.nii.ac.jp/crid/1360861704789933824","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Brownian ratchet force sensor at the contacting point between F-actin barbed end and lamellipodium tip plasma membrane"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204900779136","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"<b>Perikaryal proteins that react with an antibody against the 220 K component of axonal </b><b>neurofilaments </b>"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679877474048","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"<b>DIFFERENCES IN THE NEUROFILAMENT PROTEINS BETWEEN THE PERIKARYON AND AXON OF THE BOVINE SPINAL </b><b>GANGLION </b>"}]},{"@id":"https://cir.nii.ac.jp/crid/1571980075770171008","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Neurofilaments in health and disease"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1083/jcb.98.4.1523"},{"@type":"CIA","@value":"30017404816"},{"@type":"CROSSREF","@value":"10.2220/biomedres.6.339_references_DOI_J5QFZ2sm6w3uoOvWnMoBLmi1Lzx"},{"@type":"CROSSREF","@value":"10.1016/j.yexcr.2007.04.010_references_DOI_J5QFZ2sm6w3uoOvWnMoBLmi1Lzx"},{"@type":"CROSSREF","@value":"10.1093/jmicro/dfr051_references_DOI_J5QFZ2sm6w3uoOvWnMoBLmi1Lzx"},{"@type":"CROSSREF","@value":"10.1016/j.canlet.2014.12.030_references_DOI_J5QFZ2sm6w3uoOvWnMoBLmi1Lzx"},{"@type":"CROSSREF","@value":"10.1007/978-1-4419-6787-9_10_references_DOI_J5QFZ2sm6w3uoOvWnMoBLmi1Lzx"},{"@type":"CROSSREF","@value":"10.2220/biomedres.5.459_references_DOI_J5QFZ2sm6w3uoOvWnMoBLmi1Lzx"},{"@type":"CROSSREF","@value":"10.1016/b978-0-323-89911-6.00015-7_references_DOI_J5QFZ2sm6w3uoOvWnMoBLmi1Lzx"},{"@type":"CROSSREF","@value":"10.1097/mpa.0000000000000427_references_DOI_J5QFZ2sm6w3uoOvWnMoBLmi1Lzx"}]}